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Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study.
Am J Trop Med Hyg. 2006 Sep; 75(3):542-5.AJ

Abstract

The aims of this study were to determine the diagnostic accuracy and clinical usefulness of using nested polymerase chain reaction (PCR) for the diagnosis of scrub typhus through a prospective comparison of nested PCR and indirect immunofluorescent antibody assay (IFA). We conducted a multi-center prospective study of patients who were suffering with possible scrub typhus infection. Whole blood samples were collected for PCR testing, and sera were obtained for serology evaluation using the indirect IFA and the passive hemagglutination assay (PHA). We prospectively studied 135 patients with possible scrub typhus. One hundred eighteen patients were confirmed as having scrub typhus, 7 patients were undetermined, and 10 patients were confirmed as having other diseases. The results of nested PCR assay showed a sensitivity of 82.2% and a specificity of 100%. Ninety-six of the 118 patients were positive for IgM on their admission day. Of the 22 patients who were negative for IgM antibody at admission, 19 had positive results for nested PCR of the buffy coat. The nested PCR assay of the buffy coat is useful as a rapid and reliable test for confirming the diagnosis of scrub typhus.

Authors+Show Affiliations

Division of Infectious Diseases, Department of Internal Medicine, Chosun University College of Medicine, Gwangju, Korea. drongkim@chosun.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16968938

Citation

Kim, Dong-Min, et al. "Usefulness of Nested PCR for the Diagnosis of Scrub Typhus in Clinical Practice: a Prospective Study." The American Journal of Tropical Medicine and Hygiene, vol. 75, no. 3, 2006, pp. 542-5.
Kim DM, Yun NR, Yang TY, et al. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study. Am J Trop Med Hyg. 2006;75(3):542-5.
Kim, D. M., Yun, N. R., Yang, T. Y., Lee, J. H., Yang, J. T., Shim, S. K., Choi, E. N., Park, M. Y., & Lee, S. H. (2006). Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study. The American Journal of Tropical Medicine and Hygiene, 75(3), 542-5.
Kim DM, et al. Usefulness of Nested PCR for the Diagnosis of Scrub Typhus in Clinical Practice: a Prospective Study. Am J Trop Med Hyg. 2006;75(3):542-5. PubMed PMID: 16968938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study. AU - Kim,Dong-Min, AU - Yun,Na Ra, AU - Yang,Tae Young, AU - Lee,Ji Hyun, AU - Yang,Jong Tae, AU - Shim,Soo-Kyoung, AU - Choi,Eun-Na, AU - Park,Mi-Yeoun, AU - Lee,Seung-Hyun, PY - 2006/9/14/pubmed PY - 2006/10/26/medline PY - 2006/9/14/entrez SP - 542 EP - 5 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 75 IS - 3 N2 - The aims of this study were to determine the diagnostic accuracy and clinical usefulness of using nested polymerase chain reaction (PCR) for the diagnosis of scrub typhus through a prospective comparison of nested PCR and indirect immunofluorescent antibody assay (IFA). We conducted a multi-center prospective study of patients who were suffering with possible scrub typhus infection. Whole blood samples were collected for PCR testing, and sera were obtained for serology evaluation using the indirect IFA and the passive hemagglutination assay (PHA). We prospectively studied 135 patients with possible scrub typhus. One hundred eighteen patients were confirmed as having scrub typhus, 7 patients were undetermined, and 10 patients were confirmed as having other diseases. The results of nested PCR assay showed a sensitivity of 82.2% and a specificity of 100%. Ninety-six of the 118 patients were positive for IgM on their admission day. Of the 22 patients who were negative for IgM antibody at admission, 19 had positive results for nested PCR of the buffy coat. The nested PCR assay of the buffy coat is useful as a rapid and reliable test for confirming the diagnosis of scrub typhus. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/16968938/Usefulness_of_nested_PCR_for_the_diagnosis_of_scrub_typhus_in_clinical_practice:_A_prospective_study_ L2 - http://www.diseaseinfosearch.org/result/7277 DB - PRIME DP - Unbound Medicine ER -