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Disruption of epithelial tight junctions by yeast enhances the paracellular delivery of a model protein.
Pharm Res. 2007 Jan; 24(1):37-47.PR

Abstract

PURPOSE

The aim of this study was to investigate the effect of heat-killed yeast cells on the integrity of epithelial tight junctions in vitro.

METHODS

Changes in barrier potential of Caco-2 cell monolayers were assessed by transepithelial electrical resistance (TEER) measurements and by an increasing permeability to a marker protein, horse-radish peroxidase (HRP). Visualisation of tight junction disruption was carried out directly through electron microscopy and indirectly through fluorescence confocal microscopy and immunoblotting of the tight junction-associated proteins zonula occludens ZO-1, occludin and actin.

RESULTS

Yeast cells opened tight junctions in a reversible dose- and time-dependent manner, as shown by a decrease in TEER and an increase in HRP permeability. These changes to barrier potential were shown not to be due to cytotoxic effects but due to modulation of the tight junctions. ZO-1, actin and occludin proteins were demonstrated to be involved in yeast-induced tight junction opening through the use of confocal microscopy and western blotting. Electron microscopy confirmed a direct opening of tight junctions after application of yeast.

CONCLUSION

Yeast modulated epithelial tight junctions in a reversible manner by contraction of the actin cytoskeleton and shift of ZO-1 and occludin tight junction proteins from the membrane to cytoskeletal areas of the cell.

Authors+Show Affiliations

School of Biochemistry and Microbiology, Garstang Building, University of Leeds, Leeds, LS2 9JT, UK. fulleremily@yahoo.co.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16969693

Citation

Fuller, Emily, et al. "Disruption of Epithelial Tight Junctions By Yeast Enhances the Paracellular Delivery of a Model Protein." Pharmaceutical Research, vol. 24, no. 1, 2007, pp. 37-47.
Fuller E, Duckham C, Wood E. Disruption of epithelial tight junctions by yeast enhances the paracellular delivery of a model protein. Pharm Res. 2007;24(1):37-47.
Fuller, E., Duckham, C., & Wood, E. (2007). Disruption of epithelial tight junctions by yeast enhances the paracellular delivery of a model protein. Pharmaceutical Research, 24(1), 37-47.
Fuller E, Duckham C, Wood E. Disruption of Epithelial Tight Junctions By Yeast Enhances the Paracellular Delivery of a Model Protein. Pharm Res. 2007;24(1):37-47. PubMed PMID: 16969693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disruption of epithelial tight junctions by yeast enhances the paracellular delivery of a model protein. AU - Fuller,Emily, AU - Duckham,Craig, AU - Wood,Edward, Y1 - 2006/09/13/ PY - 2006/05/20/received PY - 2006/07/17/accepted PY - 2006/9/14/pubmed PY - 2007/1/27/medline PY - 2006/9/14/entrez SP - 37 EP - 47 JF - Pharmaceutical research JO - Pharm Res VL - 24 IS - 1 N2 - PURPOSE: The aim of this study was to investigate the effect of heat-killed yeast cells on the integrity of epithelial tight junctions in vitro. METHODS: Changes in barrier potential of Caco-2 cell monolayers were assessed by transepithelial electrical resistance (TEER) measurements and by an increasing permeability to a marker protein, horse-radish peroxidase (HRP). Visualisation of tight junction disruption was carried out directly through electron microscopy and indirectly through fluorescence confocal microscopy and immunoblotting of the tight junction-associated proteins zonula occludens ZO-1, occludin and actin. RESULTS: Yeast cells opened tight junctions in a reversible dose- and time-dependent manner, as shown by a decrease in TEER and an increase in HRP permeability. These changes to barrier potential were shown not to be due to cytotoxic effects but due to modulation of the tight junctions. ZO-1, actin and occludin proteins were demonstrated to be involved in yeast-induced tight junction opening through the use of confocal microscopy and western blotting. Electron microscopy confirmed a direct opening of tight junctions after application of yeast. CONCLUSION: Yeast modulated epithelial tight junctions in a reversible manner by contraction of the actin cytoskeleton and shift of ZO-1 and occludin tight junction proteins from the membrane to cytoskeletal areas of the cell. SN - 0724-8741 UR - https://www.unboundmedicine.com/medline/citation/16969693/Disruption_of_epithelial_tight_junctions_by_yeast_enhances_the_paracellular_delivery_of_a_model_protein_ L2 - https://doi.org/10.1007/s11095-006-9124-0 DB - PRIME DP - Unbound Medicine ER -