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Molecular cytogenetic characterization and disease resistance observation of wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives.
Hereditas. 2005 Feb; 142(2005):80-5.H

Abstract

A wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives were analyzed by molecular cytological observation and tested for disease resistance in order to evaluate the potential use of the D. breviaristatum for wheat improvement. A fertility-improved partial amphiploid, TDH-2, was produced from the selfing population of Triticum aestivum cv. Chinese spring (CS)-D. breviaristatum amphiploid. Based on the results obtained from genomic in situ hybridization (GISH) and seed protein electrophoresis, we found the presence of fourteen D. breviaristatum chromosomes and the absence of D genome in TDH-2, indicating that the genomic composition of TDH-2 was AABBV(b)V(b). GISH analysis on BC(1)F(4) progenies of TDH-2xwheat demonstrated that alien D. breviaristatum chromosomes or segments were frequently transmitted. A survey of diseases resistance revealed that powdery mildew resistance from D. breviaristatum was totally expressed, however, the expression of stripe rust resistance from D. breviaristatum was dependent on the wheat background. The comparison of polymerase chain reaction (PCR), which was carried out using molecular marker SCAR(1400) linked to Pm21 D. villosum-derived powdery mildew resistance gene, suggested that D. breviaristatum possessed new resistance gene(s) different from that in D. villosum. The present study showed that the partial amphiploid TDH-2 and its derivatives could serve as novel sources for transfer of disease resistance genes to wheat.

Authors+Show Affiliations

Yang ZJ
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, PR China. yangzujun@uestc.edu.cn
Li GR
No affiliation info available
Feng J
No affiliation info available
Jiang HR
No affiliation info available
Ren ZL
No affiliation info available

MeSH

Chromosomes, PlantCytogeneticsElectrophoresis, Polyacrylamide GelFertilityGenome, PlantGliadinHybrid VigorHybridization, GeneticImmunity, InnateIn Situ Hybridization, FluorescenceKaryotypingPlant DiseasesPlant ProteinsPloidiesPoaceaeSeedsTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16970616

Citation

Yang, Zu-Jun, et al. "Molecular Cytogenetic Characterization and Disease Resistance Observation of wheat-Dasypyrum Breviaristatum Partial Amphiploid and Its Derivatives." Hereditas, vol. 142, no. 2005, 2005, pp. 80-5.
Yang ZJ, Li GR, Feng J, et al. Molecular cytogenetic characterization and disease resistance observation of wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives. Hereditas. 2005;142(2005):80-5.
Yang, Z. J., Li, G. R., Feng, J., Jiang, H. R., & Ren, Z. L. (2005). Molecular cytogenetic characterization and disease resistance observation of wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives. Hereditas, 142(2005), 80-5.
Yang ZJ, et al. Molecular Cytogenetic Characterization and Disease Resistance Observation of wheat-Dasypyrum Breviaristatum Partial Amphiploid and Its Derivatives. Hereditas. 2005;142(2005):80-5. PubMed PMID: 16970616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization and disease resistance observation of wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives. AU - Yang,Zu-Jun, AU - Li,Guang-Rong, AU - Feng,Juan, AU - Jiang,Hua-Ren, AU - Ren,Zheng-Long, PY - 2006/9/15/pubmed PY - 2006/10/19/medline PY - 2006/9/15/entrez SP - 80 EP - 5 JF - Hereditas JO - Hereditas VL - 142 IS - 2005 N2 - A wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives were analyzed by molecular cytological observation and tested for disease resistance in order to evaluate the potential use of the D. breviaristatum for wheat improvement. A fertility-improved partial amphiploid, TDH-2, was produced from the selfing population of Triticum aestivum cv. Chinese spring (CS)-D. breviaristatum amphiploid. Based on the results obtained from genomic in situ hybridization (GISH) and seed protein electrophoresis, we found the presence of fourteen D. breviaristatum chromosomes and the absence of D genome in TDH-2, indicating that the genomic composition of TDH-2 was AABBV(b)V(b). GISH analysis on BC(1)F(4) progenies of TDH-2xwheat demonstrated that alien D. breviaristatum chromosomes or segments were frequently transmitted. A survey of diseases resistance revealed that powdery mildew resistance from D. breviaristatum was totally expressed, however, the expression of stripe rust resistance from D. breviaristatum was dependent on the wheat background. The comparison of polymerase chain reaction (PCR), which was carried out using molecular marker SCAR(1400) linked to Pm21 D. villosum-derived powdery mildew resistance gene, suggested that D. breviaristatum possessed new resistance gene(s) different from that in D. villosum. The present study showed that the partial amphiploid TDH-2 and its derivatives could serve as novel sources for transfer of disease resistance genes to wheat. SN - 1601-5223 UR - https://www.unboundmedicine.com/medline/citation/16970616/Molecular_cytogenetic_characterization_and_disease_resistance_observation_of_wheat_Dasypyrum_breviaristatum_partial_amphiploid_and_its_derivatives_ L2 - https://doi.org/10.1111/j.1601-5223.2005.01918.x DB - PRIME DP - Unbound Medicine ER -