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Ag/SiO2 core-shell nanoparticle-based surface-enhanced Raman probes for immunoassay of cancer marker using silica-coated magnetic nanoparticles as separation tools.
Biosens Bioelectron. 2007 Feb 15; 22(7):1501-7.BB

Abstract

A simple, sensitive and highly specific immunoassay has been developed based on surface-enhanced Raman scattering for human alpha-fetoprotein (AFP), a tumor marker for the diagnosis of hepatocellular carcinoma. This strategy combines the Ag/SiO2 core-shell nanoparticles embedded with rhodamine B isothiocyanate dye molecules as Raman tags and the amino group modified silica-coated magnetic nanoparticle as immobilization matrix and separation tool. In the proposed system, a sandwich-type immunoassay was performed between polyclonal antibody functionalized Ag/SiO2 nanoparticle-based Raman tags and monoclonal antibody modified silica-coated magnetic nanoparticles. The presence of the analyte and the reaction between the antigen and antibody can be monitored by the Raman spectra of the Ag/SiO2 tags. Compared to the previous surface-enhanced Raman immunoassays, the main advantage of this strategy lies in two aspects. One is the high stability of Raman tags derived from the silica shell-coated silver core-shell nanostructure. The other is the use of silica-coated magnetic nanoparticles as immobilization matrix and separation tool, thus avoiding complicated pretreatment and washing steps. We have studied in detail the experimental parameters such as the effects of the antibody concentration modified on the Raman tags and on the magnetic particles, and the immunoreaction time. Using this strategy, concentration of human AFP up to 0.12 microg/ml was detected with a detection limit of 11.5 pg/ml.

Authors+Show Affiliations

State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16971110

Citation

Gong, Ji-Lai, et al. "Ag/SiO2 Core-shell Nanoparticle-based Surface-enhanced Raman Probes for Immunoassay of Cancer Marker Using Silica-coated Magnetic Nanoparticles as Separation Tools." Biosensors & Bioelectronics, vol. 22, no. 7, 2007, pp. 1501-7.
Gong JL, Liang Y, Huang Y, et al. Ag/SiO2 core-shell nanoparticle-based surface-enhanced Raman probes for immunoassay of cancer marker using silica-coated magnetic nanoparticles as separation tools. Biosens Bioelectron. 2007;22(7):1501-7.
Gong, J. L., Liang, Y., Huang, Y., Chen, J. W., Jiang, J. H., Shen, G. L., & Yu, R. Q. (2007). Ag/SiO2 core-shell nanoparticle-based surface-enhanced Raman probes for immunoassay of cancer marker using silica-coated magnetic nanoparticles as separation tools. Biosensors & Bioelectronics, 22(7), 1501-7.
Gong JL, et al. Ag/SiO2 Core-shell Nanoparticle-based Surface-enhanced Raman Probes for Immunoassay of Cancer Marker Using Silica-coated Magnetic Nanoparticles as Separation Tools. Biosens Bioelectron. 2007 Feb 15;22(7):1501-7. PubMed PMID: 16971110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ag/SiO2 core-shell nanoparticle-based surface-enhanced Raman probes for immunoassay of cancer marker using silica-coated magnetic nanoparticles as separation tools. AU - Gong,Ji-Lai, AU - Liang,Yi, AU - Huang,Yong, AU - Chen,Ji-Wei, AU - Jiang,Jian-Hui, AU - Shen,Guo-Li, AU - Yu,Ru-Qin, Y1 - 2006/09/12/ PY - 2006/01/25/received PY - 2006/07/03/revised PY - 2006/07/05/accepted PY - 2006/9/15/pubmed PY - 2007/4/6/medline PY - 2006/9/15/entrez SP - 1501 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 7 N2 - A simple, sensitive and highly specific immunoassay has been developed based on surface-enhanced Raman scattering for human alpha-fetoprotein (AFP), a tumor marker for the diagnosis of hepatocellular carcinoma. This strategy combines the Ag/SiO2 core-shell nanoparticles embedded with rhodamine B isothiocyanate dye molecules as Raman tags and the amino group modified silica-coated magnetic nanoparticle as immobilization matrix and separation tool. In the proposed system, a sandwich-type immunoassay was performed between polyclonal antibody functionalized Ag/SiO2 nanoparticle-based Raman tags and monoclonal antibody modified silica-coated magnetic nanoparticles. The presence of the analyte and the reaction between the antigen and antibody can be monitored by the Raman spectra of the Ag/SiO2 tags. Compared to the previous surface-enhanced Raman immunoassays, the main advantage of this strategy lies in two aspects. One is the high stability of Raman tags derived from the silica shell-coated silver core-shell nanostructure. The other is the use of silica-coated magnetic nanoparticles as immobilization matrix and separation tool, thus avoiding complicated pretreatment and washing steps. We have studied in detail the experimental parameters such as the effects of the antibody concentration modified on the Raman tags and on the magnetic particles, and the immunoreaction time. Using this strategy, concentration of human AFP up to 0.12 microg/ml was detected with a detection limit of 11.5 pg/ml. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16971110/Ag/SiO2_core_shell_nanoparticle_based_surface_enhanced_Raman_probes_for_immunoassay_of_cancer_marker_using_silica_coated_magnetic_nanoparticles_as_separation_tools_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00313-7 DB - PRIME DP - Unbound Medicine ER -