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Cadmium induced mitochondrial injury and apoptosis in vero cells: protective effect of diallyl tetrasufide from garlic.

Abstract

Oxidative stress and mitochondrial injury has been implicated in cadmium-induced apoptosis. In this study, we examined the protective effect of diallyl tetrasulfide from garlic on cadmium induced oxidative stress and apoptosis in vero cells. Exposure of vero cells to cadmium (10 microM) for 18 h showed the apoptotic events such as loss of cell viability, alterations in nuclear morphology and decreased mitochondrial membrane potential with significantly increased levels of reactive oxygen species (super oxide anion and hydrogen peroxide). Treatment of vero cells with cadmium (10 microM) and diallyl tetrasulfide (5-50 microg/ml) showed that diallyl tetrasulfide attenuated the cadmium-induced suppression of cell viability in a dose dependent manner and highly significant effect was observed at 40 microg/ml. The nuclei morphological analysis with 4',6-diamidino-2-phenylindole staining confirmed that diallyl tetrasulfide at 40 microg/ml prevented the Cd (10 microM) induced apoptosis. Flow cytometric analysis with 2',7'-dichlorofluorencein diacetate showed that the inhibitory effect of diallyl tetrasulfide (10-40 microg/ml) on reactive oxygen species generation parallel with its effect on cell viability. In addition, diallyl tetrasulfide (40 microg/ml) remarkably reduced the cadmium-induced accumulation of superoxide radical and hydrogen peroxide with in cells. Further, diallyl tetrasulfide significantly protected the cadmium-induced decrease in mitochondrial membrane potential, an indicator of mitochondrial function. Our study suggest that diallyl tetrasulfide affect the reactive oxygen species generation induced by cadmium, and possesses a novel protective effect on the cytolethality associated with mitochondrial injury, which contributes to the antiapoptotic effect of diallyl tetrasulfide against cadmium.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India.

    , , ,

    Source

    MeSH

    Allyl Compounds
    Animals
    Apoptosis
    Cadmium
    Cell Nucleus
    Cercopithecus aethiops
    Dose-Response Relationship, Drug
    Garlic
    Membrane Potential, Mitochondrial
    Mitochondria
    Oxidative Stress
    Reactive Oxygen Species
    Sulfides
    Vero Cells

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    16971165

    Citation

    Murugavel, Ponnusamy, et al. "Cadmium Induced Mitochondrial Injury and Apoptosis in Vero Cells: Protective Effect of Diallyl Tetrasufide From Garlic." The International Journal of Biochemistry & Cell Biology, vol. 39, no. 1, 2007, pp. 161-70.
    Murugavel P, Pari L, Sitasawad SL, et al. Cadmium induced mitochondrial injury and apoptosis in vero cells: protective effect of diallyl tetrasufide from garlic. Int J Biochem Cell Biol. 2007;39(1):161-70.
    Murugavel, P., Pari, L., Sitasawad, S. L., Kumar, S., & Kumar, S. (2007). Cadmium induced mitochondrial injury and apoptosis in vero cells: protective effect of diallyl tetrasufide from garlic. The International Journal of Biochemistry & Cell Biology, 39(1), pp. 161-70.
    Murugavel P, et al. Cadmium Induced Mitochondrial Injury and Apoptosis in Vero Cells: Protective Effect of Diallyl Tetrasufide From Garlic. Int J Biochem Cell Biol. 2007;39(1):161-70. PubMed PMID: 16971165.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Cadmium induced mitochondrial injury and apoptosis in vero cells: protective effect of diallyl tetrasufide from garlic. AU - Murugavel,Ponnusamy, AU - Pari,Leelavinothan, AU - Sitasawad,Sandhya L, AU - Kumar,Sandeep, AU - Kumar,Santhosh, Y1 - 2006/08/18/ PY - 2006/04/18/received PY - 2006/07/21/revised PY - 2006/07/27/accepted PY - 2006/9/15/pubmed PY - 2007/2/3/medline PY - 2006/9/15/entrez SP - 161 EP - 70 JF - The international journal of biochemistry & cell biology JO - Int. J. Biochem. Cell Biol. VL - 39 IS - 1 N2 - Oxidative stress and mitochondrial injury has been implicated in cadmium-induced apoptosis. In this study, we examined the protective effect of diallyl tetrasulfide from garlic on cadmium induced oxidative stress and apoptosis in vero cells. Exposure of vero cells to cadmium (10 microM) for 18 h showed the apoptotic events such as loss of cell viability, alterations in nuclear morphology and decreased mitochondrial membrane potential with significantly increased levels of reactive oxygen species (super oxide anion and hydrogen peroxide). Treatment of vero cells with cadmium (10 microM) and diallyl tetrasulfide (5-50 microg/ml) showed that diallyl tetrasulfide attenuated the cadmium-induced suppression of cell viability in a dose dependent manner and highly significant effect was observed at 40 microg/ml. The nuclei morphological analysis with 4',6-diamidino-2-phenylindole staining confirmed that diallyl tetrasulfide at 40 microg/ml prevented the Cd (10 microM) induced apoptosis. Flow cytometric analysis with 2',7'-dichlorofluorencein diacetate showed that the inhibitory effect of diallyl tetrasulfide (10-40 microg/ml) on reactive oxygen species generation parallel with its effect on cell viability. In addition, diallyl tetrasulfide (40 microg/ml) remarkably reduced the cadmium-induced accumulation of superoxide radical and hydrogen peroxide with in cells. Further, diallyl tetrasulfide significantly protected the cadmium-induced decrease in mitochondrial membrane potential, an indicator of mitochondrial function. Our study suggest that diallyl tetrasulfide affect the reactive oxygen species generation induced by cadmium, and possesses a novel protective effect on the cytolethality associated with mitochondrial injury, which contributes to the antiapoptotic effect of diallyl tetrasulfide against cadmium. SN - 1357-2725 UR - https://www.unboundmedicine.com/medline/citation/16971165/Cadmium_induced_mitochondrial_injury_and_apoptosis_in_vero_cells:_protective_effect_of_diallyl_tetrasufide_from_garlic_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1357-2725(06)00229-9 DB - PRIME DP - Unbound Medicine ER -