Tags

Type your tag names separated by a space and hit enter

Single hair analysis of methamphetamine and amphetamine by solid phase microextraction coupled with in matrix derivatization.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Oct 02; 842(2):106-10.JC

Abstract

A sensitive method for detection of methamphetamine (MA) and amphetamine (AP) in human hair was developed using solid phase microextraction (SPME) and one-pot derivatization. MA and AP were directly derivatized to N-propoxycarbonyl derivatives in an aqueous solution by propylchloroformate in a one-pot reaction before extraction by SPME. The derivatives were extracted to a coating of SPME from a headspace of the vial. The adsorbed derivatives were thermally desorbed in the injection port of a gas chromatograph. Pentadeuterated MA was used as an internal standard. The absolute recoveries of MA and AP from the spiked hair were 2.80-17.5%, respectively. The calibration curves showed linearity in the range of 0.05-20 ng/0.08 mg/vial for MA and 0.1-20 ng/0.08 mg/vial for AP in hair. Detection limits (S/N = 3) of MA and AP were 0.02 and 0.05 ng/0.08 mg/vial. The coefficients of variation of intraday were 1.04-26.4%. Additionally, this proposed method was applied to segmental analysis in clinical and medico-legal cases of MA intoxication.

Authors+Show Affiliations

Department of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8551, Japan. nishidam@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16971192

Citation

Nishida, Manami, et al. "Single Hair Analysis of Methamphetamine and Amphetamine By Solid Phase Microextraction Coupled With in Matrix Derivatization." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 842, no. 2, 2006, pp. 106-10.
Nishida M, Yashiki M, Namera A, et al. Single hair analysis of methamphetamine and amphetamine by solid phase microextraction coupled with in matrix derivatization. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;842(2):106-10.
Nishida, M., Yashiki, M., Namera, A., & Kimura, K. (2006). Single hair analysis of methamphetamine and amphetamine by solid phase microextraction coupled with in matrix derivatization. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 842(2), 106-10.
Nishida M, et al. Single Hair Analysis of Methamphetamine and Amphetamine By Solid Phase Microextraction Coupled With in Matrix Derivatization. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Oct 2;842(2):106-10. PubMed PMID: 16971192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single hair analysis of methamphetamine and amphetamine by solid phase microextraction coupled with in matrix derivatization. AU - Nishida,Manami, AU - Yashiki,Mikio, AU - Namera,Akira, AU - Kimura,Kojiro, Y1 - 2006/09/12/ PY - 2005/12/19/received PY - 2006/06/07/revised PY - 2006/07/15/accepted PY - 2006/9/15/pubmed PY - 2006/12/23/medline PY - 2006/9/15/entrez SP - 106 EP - 10 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 842 IS - 2 N2 - A sensitive method for detection of methamphetamine (MA) and amphetamine (AP) in human hair was developed using solid phase microextraction (SPME) and one-pot derivatization. MA and AP were directly derivatized to N-propoxycarbonyl derivatives in an aqueous solution by propylchloroformate in a one-pot reaction before extraction by SPME. The derivatives were extracted to a coating of SPME from a headspace of the vial. The adsorbed derivatives were thermally desorbed in the injection port of a gas chromatograph. Pentadeuterated MA was used as an internal standard. The absolute recoveries of MA and AP from the spiked hair were 2.80-17.5%, respectively. The calibration curves showed linearity in the range of 0.05-20 ng/0.08 mg/vial for MA and 0.1-20 ng/0.08 mg/vial for AP in hair. Detection limits (S/N = 3) of MA and AP were 0.02 and 0.05 ng/0.08 mg/vial. The coefficients of variation of intraday were 1.04-26.4%. Additionally, this proposed method was applied to segmental analysis in clinical and medico-legal cases of MA intoxication. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16971192/Single_hair_analysis_of_methamphetamine_and_amphetamine_by_solid_phase_microextraction_coupled_with_in_matrix_derivatization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00580-0 DB - PRIME DP - Unbound Medicine ER -