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Simple and sensitive determination of Delta(9)-tetrahydrocannabinol, cannabidiol and cannabinol in hair by combined silylation, headspace solid phase microextraction and gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 01; 846(1-2):78-85.JC

Abstract

A new method for determination of Delta(9)-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair based on alkaline hair hydrolysis, extraction by iso-octane, combined derivatization with N,O-bis-(trimethylsilyl)-trifluoroacetamide and headspace solid phase microextraction of the extract residue, and gas chromatography-mass spectrometry was developed and evaluated. The limits of detection of the three compounds were 0.01-0.02 ng/mg. The method was routinely applied to more than 250 hair samples. In 77 positive samples, the concentrations ranged from LOD to 4.2 ng/mg for THC (mean 0.49 ng/mg), to 12.1 ng/mg for CBD (mean 0.37 ng/mg) and to 0.85 ng/mg for CBN (mean 0.12 ng/mg) using a sample amount of 30 mg. The frequently observed increase of the segmental drug concentrations from proximal to distal is explained by progressive accumulation in the hair shaft from sebum or side stream smoke.

Authors+Show Affiliations

Institute of Legal Medicine, University Hospital Charité, 14195 Berlin, Hittorfstr. 18, Germany.No affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16971195

Citation

Nadulski, Thomas, and Fritz Pragst. "Simple and Sensitive Determination of Delta(9)-tetrahydrocannabinol, Cannabidiol and Cannabinol in Hair By Combined Silylation, Headspace Solid Phase Microextraction and Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 846, no. 1-2, 2007, pp. 78-85.
Nadulski T, Pragst F. Simple and sensitive determination of Delta(9)-tetrahydrocannabinol, cannabidiol and cannabinol in hair by combined silylation, headspace solid phase microextraction and gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;846(1-2):78-85.
Nadulski, T., & Pragst, F. (2007). Simple and sensitive determination of Delta(9)-tetrahydrocannabinol, cannabidiol and cannabinol in hair by combined silylation, headspace solid phase microextraction and gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 846(1-2), 78-85.
Nadulski T, Pragst F. Simple and Sensitive Determination of Delta(9)-tetrahydrocannabinol, Cannabidiol and Cannabinol in Hair By Combined Silylation, Headspace Solid Phase Microextraction and Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):78-85. PubMed PMID: 16971195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple and sensitive determination of Delta(9)-tetrahydrocannabinol, cannabidiol and cannabinol in hair by combined silylation, headspace solid phase microextraction and gas chromatography-mass spectrometry. AU - Nadulski,Thomas, AU - Pragst,Fritz, Y1 - 2006/09/12/ PY - 2006/06/19/received PY - 2006/08/01/revised PY - 2006/08/11/accepted PY - 2006/9/15/pubmed PY - 2007/4/3/medline PY - 2006/9/15/entrez SP - 78 EP - 85 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 846 IS - 1-2 N2 - A new method for determination of Delta(9)-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair based on alkaline hair hydrolysis, extraction by iso-octane, combined derivatization with N,O-bis-(trimethylsilyl)-trifluoroacetamide and headspace solid phase microextraction of the extract residue, and gas chromatography-mass spectrometry was developed and evaluated. The limits of detection of the three compounds were 0.01-0.02 ng/mg. The method was routinely applied to more than 250 hair samples. In 77 positive samples, the concentrations ranged from LOD to 4.2 ng/mg for THC (mean 0.49 ng/mg), to 12.1 ng/mg for CBD (mean 0.37 ng/mg) and to 0.85 ng/mg for CBN (mean 0.12 ng/mg) using a sample amount of 30 mg. The frequently observed increase of the segmental drug concentrations from proximal to distal is explained by progressive accumulation in the hair shaft from sebum or side stream smoke. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16971195/Simple_and_sensitive_determination_of_Delta_9__tetrahydrocannabinol_cannabidiol_and_cannabinol_in_hair_by_combined_silylation_headspace_solid_phase_microextraction_and_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00679-9 DB - PRIME DP - Unbound Medicine ER -