Tags

Type your tag names separated by a space and hit enter

Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.
Prep Biochem Biotechnol. 2006; 36(4):287-96.PB

Abstract

Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).

Links

Publisher Full Text

Authors+Show Affiliations

Stefan-van Staden RI
Faculty of Chemistry, University of Bucharest, Bucharest, Romania. iustinavanstaden@yahoo.com
Bokretsion RG
No affiliation info available
van Staden JF
No affiliation info available
Aboul-Enein HY
No affiliation info available

MeSH

AmidohydrolasesBiosensing TechniquesCarbonCreatineCreatinineEnzymes, ImmobilizedFlow Injection AnalysisHydrogen PeroxideOnline SystemsSarcosine OxidaseUreohydrolases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16971300

Citation

Stefan-van Staden, Raluca-Ioana, et al. "Simultaneous Detection of Creatine and Creatinine Using a Sequential Injection Analysis/biosensor System." Preparative Biochemistry & Biotechnology, vol. 36, no. 4, 2006, pp. 287-96.
Stefan-van Staden RI, Bokretsion RG, van Staden JF, et al. Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system. Prep Biochem Biotechnol. 2006;36(4):287-96.
Stefan-van Staden, R. I., Bokretsion, R. G., van Staden, J. F., & Aboul-Enein, H. Y. (2006). Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system. Preparative Biochemistry & Biotechnology, 36(4), 287-96.
Stefan-van Staden RI, et al. Simultaneous Detection of Creatine and Creatinine Using a Sequential Injection Analysis/biosensor System. Prep Biochem Biotechnol. 2006;36(4):287-96. PubMed PMID: 16971300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system. AU - Stefan-van Staden,Raluca-Ioana, AU - Bokretsion,Rahel Girmai, AU - van Staden,Jacobus F, AU - Aboul-Enein,Hassan Y, PY - 2006/9/15/pubmed PY - 2006/12/23/medline PY - 2006/9/15/entrez SP - 287 EP - 96 JF - Preparative biochemistry & biotechnology JO - Prep Biochem Biotechnol VL - 36 IS - 4 N2 - Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10). SN - 1082-6068 UR - https://www.unboundmedicine.com/medline/citation/16971300/Simultaneous_detection_of_creatine_and_creatinine_using_a_sequential_injection_analysis/biosensor_system_ L2 - https://www.tandfonline.com/doi/full/10.1080/10826060600912393 DB - PRIME DP - Unbound Medicine ER -