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Saliva is one likely source of leukotriene B4 in exhaled breath condensate.
Eur Respir J. 2006 Dec; 28(6):1229-35.ER

Abstract

Leukotriene (LT)B4 in exhaled breath condensate (EBC) has been reported to be elevated in airway inflammation. The origin of leukotrienes in EBC is, however, not established. The aims of this study are to measure LTB4 levels in EBC collected in two challenges characterised by a strong neutrophilic airway inflammation and to compare LTB4 levels in EBC with levels in sputum and saliva. LTB4 and alpha-amylase were measured in EBC from 34 healthy subjects exposed in a pig confinement building or to a lipopolysaccharide provocation. These markers were also measured in induced sputum in 11 of the subjects. For comparison, LTB4 and alpha-amylase were measured in saliva from healthy subjects. Only four out of 102 EBC samples had detectable LTB4 (28-100 pg x mL(-1)). alpha-amylase activity was detected in the LTB4-positive samples. In contrast, LTB4 was detected in all examined sputum supernatants in the same study (median 1,190 pg x mL(-1)). The median LTB4 level in saliva was 469 pg x mL(-1). High levels of leukotriene B4 in saliva and the presence of leukotriene B4 in exhaled breath condensate only when alpha-amylase was detected, indicate that leukotriene B4 found in exhaled breath condensate is the result of saliva contamination. As leukotriene B4 was consistently present in sputum supernatants, exhaled breath condensate may be inappropriate for monitoring airway leukotriene B4.

Authors+Show Affiliations

Unit for Experimental Asthma and Allergy Research, Division of Physiology, PO Box 287, The National Institute of Environmental Medicine, Karolinska Institutet, SE-17177 Stockholm, Sweden. Flora.Gaber@ki.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16971403

Citation

Gaber, F, et al. "Saliva Is One Likely Source of Leukotriene B4 in Exhaled Breath Condensate." The European Respiratory Journal, vol. 28, no. 6, 2006, pp. 1229-35.
Gaber F, Acevedo F, Delin I, et al. Saliva is one likely source of leukotriene B4 in exhaled breath condensate. Eur Respir J. 2006;28(6):1229-35.
Gaber, F., Acevedo, F., Delin, I., Sundblad, B. M., Palmberg, L., Larsson, K., Kumlin, M., & Dahlén, S. E. (2006). Saliva is one likely source of leukotriene B4 in exhaled breath condensate. The European Respiratory Journal, 28(6), 1229-35.
Gaber F, et al. Saliva Is One Likely Source of Leukotriene B4 in Exhaled Breath Condensate. Eur Respir J. 2006;28(6):1229-35. PubMed PMID: 16971403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saliva is one likely source of leukotriene B4 in exhaled breath condensate. AU - Gaber,F, AU - Acevedo,F, AU - Delin,I, AU - Sundblad,B-M, AU - Palmberg,L, AU - Larsson,K, AU - Kumlin,M, AU - Dahlén,S-E, Y1 - 2006/09/13/ PY - 2006/9/15/pubmed PY - 2007/3/3/medline PY - 2006/9/15/entrez SP - 1229 EP - 35 JF - The European respiratory journal JO - Eur Respir J VL - 28 IS - 6 N2 - Leukotriene (LT)B4 in exhaled breath condensate (EBC) has been reported to be elevated in airway inflammation. The origin of leukotrienes in EBC is, however, not established. The aims of this study are to measure LTB4 levels in EBC collected in two challenges characterised by a strong neutrophilic airway inflammation and to compare LTB4 levels in EBC with levels in sputum and saliva. LTB4 and alpha-amylase were measured in EBC from 34 healthy subjects exposed in a pig confinement building or to a lipopolysaccharide provocation. These markers were also measured in induced sputum in 11 of the subjects. For comparison, LTB4 and alpha-amylase were measured in saliva from healthy subjects. Only four out of 102 EBC samples had detectable LTB4 (28-100 pg x mL(-1)). alpha-amylase activity was detected in the LTB4-positive samples. In contrast, LTB4 was detected in all examined sputum supernatants in the same study (median 1,190 pg x mL(-1)). The median LTB4 level in saliva was 469 pg x mL(-1). High levels of leukotriene B4 in saliva and the presence of leukotriene B4 in exhaled breath condensate only when alpha-amylase was detected, indicate that leukotriene B4 found in exhaled breath condensate is the result of saliva contamination. As leukotriene B4 was consistently present in sputum supernatants, exhaled breath condensate may be inappropriate for monitoring airway leukotriene B4. SN - 0903-1936 UR - https://www.unboundmedicine.com/medline/citation/16971403/Saliva_is_one_likely_source_of_leukotriene_B4_in_exhaled_breath_condensate_ DB - PRIME DP - Unbound Medicine ER -