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Expression of hyperpolarization-activated cyclic nucleotide-gated cation channels in rat dorsal root ganglion neurons innervating urinary bladder.
Brain Res. 2006 Nov 13; 1119(1):115-23.BR

Abstract

Afferent pathways innervating the urinary bladder consist of myelinated Adelta- and unmyelinated C-fibers, the neuronal cell bodies of which correspond to medium and small-sized cell populations of dorsal root ganglion (DRG) neurons, respectively. Since hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel currents have been identified in various peripheral sensory neurons, we examined the expression of isoforms of HCN channels in the L6-S1 spinal cord and bladder afferent neurons from L6-S1 DRG in rats. Among HCN-1, HCN-2 and HCN-4 channel subtypes, positive staining with HCN-2 antibodies was found in the superficial dorsal horn of the spinal cord and small- and medium-sized unidentified DRG neurons. In dye-labeled bladder afferent neurons, HCN-2-positive cells were found in approximately 60% of neurons, and HCN-2 was expressed in both small- and medium-sized neurons with a higher ratio (expression ratio: 61% and 50% of neurons, respectively) compared with unidentified DRG neurons, in which the HCN expression ratio was 47% and 21% of small- and medium-sized cells, respectively. These results suggest that HCN-2 is the predominant subtype of HCN channels, which can control neuronal excitability, in small-sized C-fiber and medium-sized Adelta fiber DRG neurons including bladder afferent neurons, and might modulate activity of bladder afferent pathways controlling the micturition reflex.

Authors+Show Affiliations

Department of Urology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16979600

Citation

Matsuyoshi, Hiroko, et al. "Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channels in Rat Dorsal Root Ganglion Neurons Innervating Urinary Bladder." Brain Research, vol. 1119, no. 1, 2006, pp. 115-23.
Matsuyoshi H, Masuda N, Chancellor MB, et al. Expression of hyperpolarization-activated cyclic nucleotide-gated cation channels in rat dorsal root ganglion neurons innervating urinary bladder. Brain Res. 2006;1119(1):115-23.
Matsuyoshi, H., Masuda, N., Chancellor, M. B., Erickson, V. L., Hirao, Y., de Groat, W. C., Wanaka, A., & Yoshimura, N. (2006). Expression of hyperpolarization-activated cyclic nucleotide-gated cation channels in rat dorsal root ganglion neurons innervating urinary bladder. Brain Research, 1119(1), 115-23.
Matsuyoshi H, et al. Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channels in Rat Dorsal Root Ganglion Neurons Innervating Urinary Bladder. Brain Res. 2006 Nov 13;1119(1):115-23. PubMed PMID: 16979600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of hyperpolarization-activated cyclic nucleotide-gated cation channels in rat dorsal root ganglion neurons innervating urinary bladder. AU - Matsuyoshi,Hiroko, AU - Masuda,Noriyuki, AU - Chancellor,Michael B, AU - Erickson,Vickie L, AU - Hirao,Yoshihiko, AU - de Groat,William C, AU - Wanaka,Akio, AU - Yoshimura,Naoki, Y1 - 2006/09/18/ PY - 2006/06/15/received PY - 2006/07/25/revised PY - 2006/08/14/accepted PY - 2006/9/19/pubmed PY - 2007/1/27/medline PY - 2006/9/19/entrez SP - 115 EP - 23 JF - Brain research JO - Brain Res. VL - 1119 IS - 1 N2 - Afferent pathways innervating the urinary bladder consist of myelinated Adelta- and unmyelinated C-fibers, the neuronal cell bodies of which correspond to medium and small-sized cell populations of dorsal root ganglion (DRG) neurons, respectively. Since hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel currents have been identified in various peripheral sensory neurons, we examined the expression of isoforms of HCN channels in the L6-S1 spinal cord and bladder afferent neurons from L6-S1 DRG in rats. Among HCN-1, HCN-2 and HCN-4 channel subtypes, positive staining with HCN-2 antibodies was found in the superficial dorsal horn of the spinal cord and small- and medium-sized unidentified DRG neurons. In dye-labeled bladder afferent neurons, HCN-2-positive cells were found in approximately 60% of neurons, and HCN-2 was expressed in both small- and medium-sized neurons with a higher ratio (expression ratio: 61% and 50% of neurons, respectively) compared with unidentified DRG neurons, in which the HCN expression ratio was 47% and 21% of small- and medium-sized cells, respectively. These results suggest that HCN-2 is the predominant subtype of HCN channels, which can control neuronal excitability, in small-sized C-fiber and medium-sized Adelta fiber DRG neurons including bladder afferent neurons, and might modulate activity of bladder afferent pathways controlling the micturition reflex. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/16979600/Expression_of_hyperpolarization_activated_cyclic_nucleotide_gated_cation_channels_in_rat_dorsal_root_ganglion_neurons_innervating_urinary_bladder_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(06)02448-6 DB - PRIME DP - Unbound Medicine ER -