Tags

Type your tag names separated by a space and hit enter

Effects of immunization of Pelibuey lambs with Oestrus ovis digestive tract protein extracts on larval establishment and development.
Vet Parasitol. 2007 Jan 31; 143(2):140-6.VP

Abstract

Larval midgut proteins of hematophagous parasites contain strong antigens that can be used for host immunization. This concept has been applied for immunization of Pelibuey sheep against Oestrus ovis L. (Diptera: Oestridae). The aim of this study was to examine the effect of immunization on larval establishment (LE) and development. Immunized lambs (I, n = 6) received two injections of crude gut membrane protein extracts (GMPE) from third instar larvae with Freund's incomplete adjuvant (FIA) on days 0 (Day of first immunization) and 21 (0.4 and 0.45 mg GMPE/lamb, respectively). The control group (C, n = 5) received physiological saline with FIA. Lambs were challenged with first instars on Day 29 (20 larvae) and Day 43 (25 larvae). Blood samples were collected biweekly and IgG titers were analyzed by ELISA. All lambs were slaughtered on Day 90 and number of larvae recovered, larval stage and larval weight were recorded at necropsy. No significant effect of immunization on LE (C = 28.9%; I = 31.0% P > 0.05) was observed. Antibody titers were higher in the immunized group on Day 28 (P < 0.05), but subsequently similar in both groups. Larval physiological age and weight were also significantly (P < 0.05) affected by immunization. Immunization of Pelibuey lambs with GMPE did not affect LE but did delay O. ovis larval development.

Links

Publisher Full Text

Authors+Show Affiliations

Angulo-Valadez CE
Centro de Investigaciones Biológicas del Noroeste, PO Box 128, La Paz, B C S 23000, Mexico.
Cepeda-Palacios R
No affiliation info available
Jacquiet P
No affiliation info available
Dorchies P
No affiliation info available
Prévot F
No affiliation info available
Ascencio-Valle F
No affiliation info available
Ramirez-Orduña JM
No affiliation info available
Torres F
No affiliation info available

MeSH

AnimalsDipteraEnzyme-Linked Immunosorbent AssayFreund's AdjuvantImmunizationImmunoglobulin GLarvaMolecular WeightMyiasisSheepSheep Diseases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16979824

Citation

Angulo-Valadez, C E., et al. "Effects of Immunization of Pelibuey Lambs With Oestrus Ovis Digestive Tract Protein Extracts On Larval Establishment and Development." Veterinary Parasitology, vol. 143, no. 2, 2007, pp. 140-6.
Angulo-Valadez CE, Cepeda-Palacios R, Jacquiet P, et al. Effects of immunization of Pelibuey lambs with Oestrus ovis digestive tract protein extracts on larval establishment and development. Vet Parasitol. 2007;143(2):140-6.
Angulo-Valadez, C. E., Cepeda-Palacios, R., Jacquiet, P., Dorchies, P., Prévot, F., Ascencio-Valle, F., Ramirez-Orduña, J. M., & Torres, F. (2007). Effects of immunization of Pelibuey lambs with Oestrus ovis digestive tract protein extracts on larval establishment and development. Veterinary Parasitology, 143(2), 140-6.
Angulo-Valadez CE, et al. Effects of Immunization of Pelibuey Lambs With Oestrus Ovis Digestive Tract Protein Extracts On Larval Establishment and Development. Vet Parasitol. 2007 Jan 31;143(2):140-6. PubMed PMID: 16979824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of immunization of Pelibuey lambs with Oestrus ovis digestive tract protein extracts on larval establishment and development. AU - Angulo-Valadez,C E, AU - Cepeda-Palacios,R, AU - Jacquiet,P, AU - Dorchies,P, AU - Prévot,F, AU - Ascencio-Valle,F, AU - Ramirez-Orduña,J M, AU - Torres,F, Y1 - 2006/09/18/ PY - 2006/05/24/received PY - 2006/07/07/revised PY - 2006/08/02/accepted PY - 2006/9/19/pubmed PY - 2007/2/28/medline PY - 2006/9/19/entrez SP - 140 EP - 6 JF - Veterinary parasitology JO - Vet Parasitol VL - 143 IS - 2 N2 - Larval midgut proteins of hematophagous parasites contain strong antigens that can be used for host immunization. This concept has been applied for immunization of Pelibuey sheep against Oestrus ovis L. (Diptera: Oestridae). The aim of this study was to examine the effect of immunization on larval establishment (LE) and development. Immunized lambs (I, n = 6) received two injections of crude gut membrane protein extracts (GMPE) from third instar larvae with Freund's incomplete adjuvant (FIA) on days 0 (Day of first immunization) and 21 (0.4 and 0.45 mg GMPE/lamb, respectively). The control group (C, n = 5) received physiological saline with FIA. Lambs were challenged with first instars on Day 29 (20 larvae) and Day 43 (25 larvae). Blood samples were collected biweekly and IgG titers were analyzed by ELISA. All lambs were slaughtered on Day 90 and number of larvae recovered, larval stage and larval weight were recorded at necropsy. No significant effect of immunization on LE (C = 28.9%; I = 31.0% P > 0.05) was observed. Antibody titers were higher in the immunized group on Day 28 (P < 0.05), but subsequently similar in both groups. Larval physiological age and weight were also significantly (P < 0.05) affected by immunization. Immunization of Pelibuey lambs with GMPE did not affect LE but did delay O. ovis larval development. SN - 0304-4017 UR - https://www.unboundmedicine.com/medline/citation/16979824/Effects_of_immunization_of_Pelibuey_lambs_with_Oestrus_ovis_digestive_tract_protein_extracts_on_larval_establishment_and_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(06)00476-6 DB - PRIME DP - Unbound Medicine ER -