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Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways.
Cell Signal. 2007 Feb; 19(2):419-27.CS

Abstract

B lymphocytes are generally considered to be the only source of immunoglobulins. However, increasing evidence revealed that some human epithelial cancer cell lines, including nasopharyngeal carcinoma (NPC) cell lines, expressed immunoglobulins. Moreover, we previously found that expression of kappa light chain in NPC cells could be upregulated by EBV-encoded latent membrane protein 1 (LMP1). Here, Western blot and flow cytometric analysis of intracellular kappa staining indicated that upregulation of the expression of kappa was inhibited by using LMP1-targeted DNAzyme and that Bay11-7082 and SP600125, inhibitors of JNK and NF-kappaB, respectively, inhibited LMP1-augmented kappa light chain expression in NPC cells. LMP1-positive NPC cells expressing the dominant-negative mutant of IkappaBalpha (DNMIkappaBalpha) or of c-Jun (TAM67) exhibited significantly decreasing kappa production compared with their parental cells. These results suggest that LMP1 elevated kappa light chain through activation of the NF-kappaB and AP-1 signaling pathways. The present study provided some hints of possible mechanisms by which human cancer cells of epithelial origin produced immunoglobulins.

Authors+Show Affiliations

Cancer Research Institute, Xiangya School of Medicine, Central South University, Xiangya Road #110, Changsha, Hunan 410078, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16979873

Citation

Liu, Hai-dan, et al. "Upregulated Expression of Kappa Light Chain By Epstein-Barr Virus Encoded Latent Membrane Protein 1 in Nasopharyngeal Carcinoma Cells Via NF-kappaB and AP-1 Pathways." Cellular Signalling, vol. 19, no. 2, 2007, pp. 419-27.
Liu HD, Zheng H, Li M, et al. Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways. Cell Signal. 2007;19(2):419-27.
Liu, H. D., Zheng, H., Li, M., Hu, D. S., Tang, M., & Cao, Y. (2007). Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways. Cellular Signalling, 19(2), 419-27.
Liu HD, et al. Upregulated Expression of Kappa Light Chain By Epstein-Barr Virus Encoded Latent Membrane Protein 1 in Nasopharyngeal Carcinoma Cells Via NF-kappaB and AP-1 Pathways. Cell Signal. 2007;19(2):419-27. PubMed PMID: 16979873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways. AU - Liu,Hai-dan, AU - Zheng,Hui, AU - Li,Ming, AU - Hu,Duo-sha, AU - Tang,Min, AU - Cao,Ya, Y1 - 2006/09/18/ PY - 2006/07/13/received PY - 2006/07/24/accepted PY - 2006/9/19/pubmed PY - 2007/2/28/medline PY - 2006/9/19/entrez SP - 419 EP - 27 JF - Cellular signalling JO - Cell Signal VL - 19 IS - 2 N2 - B lymphocytes are generally considered to be the only source of immunoglobulins. However, increasing evidence revealed that some human epithelial cancer cell lines, including nasopharyngeal carcinoma (NPC) cell lines, expressed immunoglobulins. Moreover, we previously found that expression of kappa light chain in NPC cells could be upregulated by EBV-encoded latent membrane protein 1 (LMP1). Here, Western blot and flow cytometric analysis of intracellular kappa staining indicated that upregulation of the expression of kappa was inhibited by using LMP1-targeted DNAzyme and that Bay11-7082 and SP600125, inhibitors of JNK and NF-kappaB, respectively, inhibited LMP1-augmented kappa light chain expression in NPC cells. LMP1-positive NPC cells expressing the dominant-negative mutant of IkappaBalpha (DNMIkappaBalpha) or of c-Jun (TAM67) exhibited significantly decreasing kappa production compared with their parental cells. These results suggest that LMP1 elevated kappa light chain through activation of the NF-kappaB and AP-1 signaling pathways. The present study provided some hints of possible mechanisms by which human cancer cells of epithelial origin produced immunoglobulins. SN - 0898-6568 UR - https://www.unboundmedicine.com/medline/citation/16979873/Upregulated_expression_of_kappa_light_chain_by_Epstein_Barr_virus_encoded_latent_membrane_protein_1_in_nasopharyngeal_carcinoma_cells_via_NF_kappaB_and_AP_1_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0898-6568(06)00174-4 DB - PRIME DP - Unbound Medicine ER -