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Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender.
Plant Physiol. 2006 Nov; 142(3):890-900.PP

Abstract

Spike lavender (Lavandula latifolia) is an aromatic shrub cultivated worldwide for the production of essential oils. The major constituents of these oils are monoterpenes, which are obtained from isopentenyl diphosphate and dimethylallyl diphosphate precursors through the plastidial methylerythritol phosphate (MEP) pathway and/or the cytosolic mevalonate pathway. 1-Deoxy-D-xylulose-5-P synthase (DXS) catalyzes the first step of the MEP pathway. A cDNA coding for the Arabidopsis (Arabidopsis thaliana) DXS was constitutively expressed in spike lavender. Gas chromatography/mass spectrometry analyses revealed that transgenic plants accumulated significantly more essential oils compared to controls (from 101.5% to 359.0% and from 12.2% to 74.1% yield increase compared to controls in leaves and flowers, respectively). T(0) transgenic plants were grown for 2 years, self-pollinated, and the T(1) seeds obtained. The inheritance of the DXS transgene was studied in the T(1) generation. The increased essential oil phenotype observed in the transgenic T(0) plants was maintained in the progeny that inherited the DXS transgene. Total chlorophyll and carotenoid content in DXS progenies that inherited the transgene depended on the analyzed plant, showing either no variation or a significant decrease in respect to their counterparts without the transgene. Transgenic plants had a visual phenotype similar to untransformed plants (controls) in terms of morphology, growth habit, flowering, and seed germination. Our results demonstrate that the MEP pathway contributes to essential oil production in spike lavender. They also demonstrate that the DXS enzyme plays a crucial role in monoterpene precursor biosynthesis and, thus, in essential oil production in spike lavender. In addition, our results provide a strategy to increase the essential oil production in spike lavender by metabolic engineering of the MEP pathway without apparent detrimental effects on plant development and fitness.

Authors+Show Affiliations

Departamento de Biología Vegetal , Universidad de Valencia, 46100 Burjasot, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16980564

Citation

Muñoz-Bertomeu, Jesús, et al. "Up-regulation of 1-deoxy-D-xylulose-5-phosphate Synthase Enhances Production of Essential Oils in Transgenic Spike Lavender." Plant Physiology, vol. 142, no. 3, 2006, pp. 890-900.
Muñoz-Bertomeu J, Arrillaga I, Ros R, et al. Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender. Plant Physiol. 2006;142(3):890-900.
Muñoz-Bertomeu, J., Arrillaga, I., Ros, R., & Segura, J. (2006). Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender. Plant Physiology, 142(3), 890-900.
Muñoz-Bertomeu J, et al. Up-regulation of 1-deoxy-D-xylulose-5-phosphate Synthase Enhances Production of Essential Oils in Transgenic Spike Lavender. Plant Physiol. 2006;142(3):890-900. PubMed PMID: 16980564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender. AU - Muñoz-Bertomeu,Jesús, AU - Arrillaga,Isabel, AU - Ros,Roc, AU - Segura,Juan, Y1 - 2006/09/15/ PY - 2006/9/19/pubmed PY - 2007/1/27/medline PY - 2006/9/19/entrez SP - 890 EP - 900 JF - Plant physiology JO - Plant Physiol VL - 142 IS - 3 N2 - Spike lavender (Lavandula latifolia) is an aromatic shrub cultivated worldwide for the production of essential oils. The major constituents of these oils are monoterpenes, which are obtained from isopentenyl diphosphate and dimethylallyl diphosphate precursors through the plastidial methylerythritol phosphate (MEP) pathway and/or the cytosolic mevalonate pathway. 1-Deoxy-D-xylulose-5-P synthase (DXS) catalyzes the first step of the MEP pathway. A cDNA coding for the Arabidopsis (Arabidopsis thaliana) DXS was constitutively expressed in spike lavender. Gas chromatography/mass spectrometry analyses revealed that transgenic plants accumulated significantly more essential oils compared to controls (from 101.5% to 359.0% and from 12.2% to 74.1% yield increase compared to controls in leaves and flowers, respectively). T(0) transgenic plants were grown for 2 years, self-pollinated, and the T(1) seeds obtained. The inheritance of the DXS transgene was studied in the T(1) generation. The increased essential oil phenotype observed in the transgenic T(0) plants was maintained in the progeny that inherited the DXS transgene. Total chlorophyll and carotenoid content in DXS progenies that inherited the transgene depended on the analyzed plant, showing either no variation or a significant decrease in respect to their counterparts without the transgene. Transgenic plants had a visual phenotype similar to untransformed plants (controls) in terms of morphology, growth habit, flowering, and seed germination. Our results demonstrate that the MEP pathway contributes to essential oil production in spike lavender. They also demonstrate that the DXS enzyme plays a crucial role in monoterpene precursor biosynthesis and, thus, in essential oil production in spike lavender. In addition, our results provide a strategy to increase the essential oil production in spike lavender by metabolic engineering of the MEP pathway without apparent detrimental effects on plant development and fitness. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/16980564/Up_regulation_of_1_deoxy_D_xylulose_5_phosphate_synthase_enhances_production_of_essential_oils_in_transgenic_spike_lavender_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=16980564 DB - PRIME DP - Unbound Medicine ER -