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EGF-induced activation of the EGF receptor does not trigger mobilization of caveolae.
Traffic. 2006 Nov; 7(11):1518-27.T

Abstract

Caveolae-dependent endocytosis has recently been proposed in the uptake of EGF receptor (EGFR) at high concentrations of ligand. Consistently, upon incubation of HEp2 and HeLa cells with methyl-beta-cyclodextrin, we observed a small inhibitory effect on endocytosis of ligated EGFR in HEp2 cells. However, immunoelectron microscopy showed the same relative amount of bound EGF localizing to caveolae on incubation with high and low concentrations of EGF, not supporting rapid recruitment of EGFR to caveolae. Live-cell microscopy furthermore demonstrated that incubating HEp2 cells with high concentrations of EGF did not increase the mobility of caveolae. By RNA-interference-mediated knockdown of clathrin heavy chain in HEp2 and HeLa cells, we found that endocytosis of EGFR was efficiently inhibited both at high and low concentrations of EGF. Our results show that caveolae are not involved in endocytosis of EGF-bound EGFR to any significant degree and that high concentrations of EGF do not further mobilize caveolae.

Authors+Show Affiliations

Institute of Pathology, Faculty of Medicine, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Centre, N-0027 Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16984407

Citation

Kazazic, Maja, et al. "EGF-induced Activation of the EGF Receptor Does Not Trigger Mobilization of Caveolae." Traffic (Copenhagen, Denmark), vol. 7, no. 11, 2006, pp. 1518-27.
Kazazic M, Roepstorff K, Johannessen LE, et al. EGF-induced activation of the EGF receptor does not trigger mobilization of caveolae. Traffic. 2006;7(11):1518-27.
Kazazic, M., Roepstorff, K., Johannessen, L. E., Pedersen, N. M., van Deurs, B., Stang, E., & Madshus, I. H. (2006). EGF-induced activation of the EGF receptor does not trigger mobilization of caveolae. Traffic (Copenhagen, Denmark), 7(11), 1518-27.
Kazazic M, et al. EGF-induced Activation of the EGF Receptor Does Not Trigger Mobilization of Caveolae. Traffic. 2006;7(11):1518-27. PubMed PMID: 16984407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EGF-induced activation of the EGF receptor does not trigger mobilization of caveolae. AU - Kazazic,Maja, AU - Roepstorff,Kirstine, AU - Johannessen,Lene E, AU - Pedersen,Nina M, AU - van Deurs,Bo, AU - Stang,Espen, AU - Madshus,Inger H, Y1 - 2006/09/19/ PY - 2006/9/21/pubmed PY - 2007/1/4/medline PY - 2006/9/21/entrez SP - 1518 EP - 27 JF - Traffic (Copenhagen, Denmark) JO - Traffic VL - 7 IS - 11 N2 - Caveolae-dependent endocytosis has recently been proposed in the uptake of EGF receptor (EGFR) at high concentrations of ligand. Consistently, upon incubation of HEp2 and HeLa cells with methyl-beta-cyclodextrin, we observed a small inhibitory effect on endocytosis of ligated EGFR in HEp2 cells. However, immunoelectron microscopy showed the same relative amount of bound EGF localizing to caveolae on incubation with high and low concentrations of EGF, not supporting rapid recruitment of EGFR to caveolae. Live-cell microscopy furthermore demonstrated that incubating HEp2 cells with high concentrations of EGF did not increase the mobility of caveolae. By RNA-interference-mediated knockdown of clathrin heavy chain in HEp2 and HeLa cells, we found that endocytosis of EGFR was efficiently inhibited both at high and low concentrations of EGF. Our results show that caveolae are not involved in endocytosis of EGF-bound EGFR to any significant degree and that high concentrations of EGF do not further mobilize caveolae. SN - 1398-9219 UR - https://www.unboundmedicine.com/medline/citation/16984407/EGF_induced_activation_of_the_EGF_receptor_does_not_trigger_mobilization_of_caveolae_ L2 - https://doi.org/10.1111/j.1600-0854.2006.00487.x DB - PRIME DP - Unbound Medicine ER -