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Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains of Saccharomyces cerevisiae.
Redox Rep. 2006; 11(4):179-84.RR

Abstract

Much has been published on the non-enzymatic antioxidant L-ascorbic acid (vitamin C), but even so its interaction with endogenous cellular defense systems has not yet been fully elucidated. Our study investigated the antioxidant activity of L-ascorbic acid in wild-type strain EG103 (SOD) Saccharomyces cerevisiae and isogenic mutant strains deficient in cytosolic superoxide dismutase (sod1delta), mitochondrial superoxide dismutase (sod2delta) or both (sod1delta sod2delta), metabolizing aerobically or anaerobically with and without the stressing agent paraquat. The results show that during both aerobic and anaerobic metabolism there was a significant increase in the survival of both wild-type S. cerevisiae cells and the mutant cells (sod1delta, sod2delta and sod1delta sod2delta) when pretreated with L-ascorbic acid before exposure to paraquat. Exposure to paraquat resulted in higher catalase activity but this significantly decreased when the cells were pre-treated with L-ascorbic acid. These results demonstrate that due to the damage caused by paraquat, the antioxidant protection of L-ascorbic acid seems to be mediated by catalase levels in yeast cells.

Authors+Show Affiliations

Laboratório de Genética Toxicológica, ULBRA, Canoas-RS, Brazil. jenifer.saffi@ulbranet.com.brNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16984741

Citation

Saffi, Jenifer, et al. "Antioxidant Activity of L-ascorbic Acid in Wild-type and Superoxide Dismutase Deficient Strains of Saccharomyces Cerevisiae." Redox Report : Communications in Free Radical Research, vol. 11, no. 4, 2006, pp. 179-84.
Saffi J, Sonego L, Varela QD, et al. Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains of Saccharomyces cerevisiae. Redox Rep. 2006;11(4):179-84.
Saffi, J., Sonego, L., Varela, Q. D., & Salvador, M. (2006). Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains of Saccharomyces cerevisiae. Redox Report : Communications in Free Radical Research, 11(4), 179-84.
Saffi J, et al. Antioxidant Activity of L-ascorbic Acid in Wild-type and Superoxide Dismutase Deficient Strains of Saccharomyces Cerevisiae. Redox Rep. 2006;11(4):179-84. PubMed PMID: 16984741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains of Saccharomyces cerevisiae. AU - Saffi,Jenifer, AU - Sonego,Luciano, AU - Varela,Queli Defaveri, AU - Salvador,Mirian, PY - 2006/9/21/pubmed PY - 2006/10/13/medline PY - 2006/9/21/entrez SP - 179 EP - 84 JF - Redox report : communications in free radical research JO - Redox Rep VL - 11 IS - 4 N2 - Much has been published on the non-enzymatic antioxidant L-ascorbic acid (vitamin C), but even so its interaction with endogenous cellular defense systems has not yet been fully elucidated. Our study investigated the antioxidant activity of L-ascorbic acid in wild-type strain EG103 (SOD) Saccharomyces cerevisiae and isogenic mutant strains deficient in cytosolic superoxide dismutase (sod1delta), mitochondrial superoxide dismutase (sod2delta) or both (sod1delta sod2delta), metabolizing aerobically or anaerobically with and without the stressing agent paraquat. The results show that during both aerobic and anaerobic metabolism there was a significant increase in the survival of both wild-type S. cerevisiae cells and the mutant cells (sod1delta, sod2delta and sod1delta sod2delta) when pretreated with L-ascorbic acid before exposure to paraquat. Exposure to paraquat resulted in higher catalase activity but this significantly decreased when the cells were pre-treated with L-ascorbic acid. These results demonstrate that due to the damage caused by paraquat, the antioxidant protection of L-ascorbic acid seems to be mediated by catalase levels in yeast cells. SN - 1743-2928 UR - https://www.unboundmedicine.com/medline/citation/16984741/Antioxidant_activity_of_L_ascorbic_acid_in_wild_type_and_superoxide_dismutase_deficient_strains_of_Saccharomyces_cerevisiae_ L2 - https://www.tandfonline.com/doi/full/10.1179/135100006X116691 DB - PRIME DP - Unbound Medicine ER -