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[Development of a gold-immunochromatography test for rapid detecting E. coli O157].
Wei Sheng Yan Jiu. 2006 Jul; 35(4):439-41.WS

Abstract

OBJECTIVE

To develop a method for rapid detecting Escherichia coli (E. coli) O157 on site.

METHODS

A colloidal gold immunochromatography test based on double-antibody sandwich assay for detecting E. coli O157 was developed. Its sensitivity and specificity were then evaluated, and its feasibility of screening food samples were evaluated by analyzing various samples added with E. coli O157.

RESULTS

Typical detecting time is less than 15 minutes per sample. The sensitivity of the test is 1 x 10(5) cfu/ml. No any cross-reaction with 30 strains of 24 species in Enterobacteriaceae (including non-O157 E. coli, Salmonella, Shigella, Proteus, Citrobacter, Enterobacter, Serratia and Yersinia), Staphylococus, Listeria, Aeromonas and Vibrios was found. The test could be used to detect E. coli O157 in various samples such as milk powder, flour, starch, coffee, biscuit, cake, jelly and juice.

CONCLUSION

The gold-immunochromatography test appears to be a rapid, convenient, specific and sensitive test for detecting E. coli O157: H7 on site.

Authors+Show Affiliations

Wang J
Institute of Health Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100025, China.
Chen WN
No affiliation info available
Hu KX
No affiliation info available
Li W
No affiliation info available

MeSH

Antibodies, MonoclonalChromatographyEscherichia coli O157Food ContaminationFood MicrobiologyGold ColloidImmunoassayReagent StripsSensitivity and Specificity

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16986519

Citation

Wang, Jing, et al. "[Development of a Gold-immunochromatography Test for Rapid Detecting E. Coli O157]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 35, no. 4, 2006, pp. 439-41.
Wang J, Chen WN, Hu KX, et al. [Development of a gold-immunochromatography test for rapid detecting E. coli O157]. Wei Sheng Yan Jiu. 2006;35(4):439-41.
Wang, J., Chen, W. N., Hu, K. X., & Li, W. (2006). [Development of a gold-immunochromatography test for rapid detecting E. coli O157]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 35(4), 439-41.
Wang J, et al. [Development of a Gold-immunochromatography Test for Rapid Detecting E. Coli O157]. Wei Sheng Yan Jiu. 2006;35(4):439-41. PubMed PMID: 16986519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development of a gold-immunochromatography test for rapid detecting E. coli O157]. AU - Wang,Jing, AU - Chen,Wei-na, AU - Hu,Kong-xin, AU - Li,Wei, PY - 2006/9/22/pubmed PY - 2010/8/20/medline PY - 2006/9/22/entrez SP - 439 EP - 41 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 35 IS - 4 N2 - OBJECTIVE: To develop a method for rapid detecting Escherichia coli (E. coli) O157 on site. METHODS: A colloidal gold immunochromatography test based on double-antibody sandwich assay for detecting E. coli O157 was developed. Its sensitivity and specificity were then evaluated, and its feasibility of screening food samples were evaluated by analyzing various samples added with E. coli O157. RESULTS: Typical detecting time is less than 15 minutes per sample. The sensitivity of the test is 1 x 10(5) cfu/ml. No any cross-reaction with 30 strains of 24 species in Enterobacteriaceae (including non-O157 E. coli, Salmonella, Shigella, Proteus, Citrobacter, Enterobacter, Serratia and Yersinia), Staphylococus, Listeria, Aeromonas and Vibrios was found. The test could be used to detect E. coli O157 in various samples such as milk powder, flour, starch, coffee, biscuit, cake, jelly and juice. CONCLUSION: The gold-immunochromatography test appears to be a rapid, convenient, specific and sensitive test for detecting E. coli O157: H7 on site. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/16986519/[Development_of_a_gold_immunochromatography_test_for_rapid_detecting_E__coli_O157]_ DB - PRIME DP - Unbound Medicine ER -