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Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.
Folia Microbiol (Praha) 1990; 35(3):177-82FM

Abstract

In order to construct plasmids bearing inducible high-copy-number phenotype, the cloning plasmid pBR322 was modified as follows: a DNA fragment containing a strong synthetic promoter (P1), synthetic lac operator (O1), DNA sequence corresponding to the RNAI/RNAII region of the Co1E1 replicon and the CAT gene transcription terminator was substituted for the 29 bp EcoRI/HindIII DNA fragment. Two types of plasmids were constructed in this way, differing in the orientation of the RNAI/RNAII fragment. Depending on the orientation these plasmids coded for RNA molecules representing either RNAI or RNAII domains. It was found that when RNAII molecules were overproduced the plasmid copy number was about 4 times higher than that of pBR322 and only negligible change in the plasmid copy-number value was observed upon overproduction of RNAI molecules.

Authors+Show Affiliations

Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1698694

Citation

Bachvarov, D, et al. "Construction of a Co1E1 Plasmid Bearing Inducible High-copy-number Phenotype." Folia Microbiologica, vol. 35, no. 3, 1990, pp. 177-82.
Bachvarov D, Jay E, Ivanov I. Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype. Folia Microbiol (Praha). 1990;35(3):177-82.
Bachvarov, D., Jay, E., & Ivanov, I. (1990). Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype. Folia Microbiologica, 35(3), pp. 177-82.
Bachvarov D, Jay E, Ivanov I. Construction of a Co1E1 Plasmid Bearing Inducible High-copy-number Phenotype. Folia Microbiol (Praha). 1990;35(3):177-82. PubMed PMID: 1698694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype. AU - Bachvarov,D, AU - Jay,E, AU - Ivanov,I, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 177 EP - 82 JF - Folia microbiologica JO - Folia Microbiol. (Praha) VL - 35 IS - 3 N2 - In order to construct plasmids bearing inducible high-copy-number phenotype, the cloning plasmid pBR322 was modified as follows: a DNA fragment containing a strong synthetic promoter (P1), synthetic lac operator (O1), DNA sequence corresponding to the RNAI/RNAII region of the Co1E1 replicon and the CAT gene transcription terminator was substituted for the 29 bp EcoRI/HindIII DNA fragment. Two types of plasmids were constructed in this way, differing in the orientation of the RNAI/RNAII fragment. Depending on the orientation these plasmids coded for RNA molecules representing either RNAI or RNAII domains. It was found that when RNAII molecules were overproduced the plasmid copy number was about 4 times higher than that of pBR322 and only negligible change in the plasmid copy-number value was observed upon overproduction of RNAI molecules. SN - 0015-5632 UR - https://www.unboundmedicine.com/medline/citation/1698694/Construction_of_a_Co1E1_plasmid_bearing_inducible_high_copy_number_phenotype_ L2 - https://www.lens.org/lens/search?q=citation_id:1698694 DB - PRIME DP - Unbound Medicine ER -