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A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli.
Mol Microbiol 2006; 62(1):120-31MM

Abstract

Escherichia coli possesses multiple routes for iron uptake. Here we present EfeU (YcdN), a novel iron acquisition system of E. coli strain Nissle 1917. Laboratory strains of E. coli such as K12 lack a functional (efeU) ycdN gene caused by a frameshift mutation. EfeU, a member of the oxidase-dependent iron transporters (OFeT), is a homologue of the iron permease Ftr1p from yeast. The ycdN gene is part of the ycdNOB tricistronic operon which is expressed in response to iron deprivation in a Fur-dependent manner. Expression of efeU resulted in improved growth of an E. coli mutant lacking all known iron-uptake systems and mediated increased iron uptake into cells. Furthermore, the presence of other divalent metal cations did not impair growth of strains expressing efeU. The EfeU protein functioned as ferrous iron permease in proteoliposomes in vitro. Topology analysis indicated that EfeU is an integral cytoplasmic membrane protein exhibiting seven transmembrane helices. Two REXXE motifs within transmembrane helices of OFeT family members are implicated in iron translocation. Site-directed mutagenesis of each REGLE motif of EfeU diminished iron uptake in vivo and growth yield. In vitro the EfeU variant protein with an altered first REGLE motif was impaired in iron permeation, whereas activity of the EfeU variant with a mutation in the second motif was similar to the wild-type protein.

Authors+Show Affiliations

Institute for Microbiology, Martin-Luther-University, Halle, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16987175

Citation

Grosse, Cornelia, et al. "A New Ferrous Iron-uptake Transporter, EfeU (YcdN), From Escherichia Coli." Molecular Microbiology, vol. 62, no. 1, 2006, pp. 120-31.
Grosse C, Scherer J, Koch D, et al. A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Mol Microbiol. 2006;62(1):120-31.
Grosse, C., Scherer, J., Koch, D., Otto, M., Taudte, N., & Grass, G. (2006). A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Molecular Microbiology, 62(1), pp. 120-31.
Grosse C, et al. A New Ferrous Iron-uptake Transporter, EfeU (YcdN), From Escherichia Coli. Mol Microbiol. 2006;62(1):120-31. PubMed PMID: 16987175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. AU - Grosse,Cornelia, AU - Scherer,Judith, AU - Koch,Doreen, AU - Otto,Markus, AU - Taudte,Nadine, AU - Grass,Gregor, PY - 2006/9/22/pubmed PY - 2007/1/24/medline PY - 2006/9/22/entrez SP - 120 EP - 31 JF - Molecular microbiology JO - Mol. Microbiol. VL - 62 IS - 1 N2 - Escherichia coli possesses multiple routes for iron uptake. Here we present EfeU (YcdN), a novel iron acquisition system of E. coli strain Nissle 1917. Laboratory strains of E. coli such as K12 lack a functional (efeU) ycdN gene caused by a frameshift mutation. EfeU, a member of the oxidase-dependent iron transporters (OFeT), is a homologue of the iron permease Ftr1p from yeast. The ycdN gene is part of the ycdNOB tricistronic operon which is expressed in response to iron deprivation in a Fur-dependent manner. Expression of efeU resulted in improved growth of an E. coli mutant lacking all known iron-uptake systems and mediated increased iron uptake into cells. Furthermore, the presence of other divalent metal cations did not impair growth of strains expressing efeU. The EfeU protein functioned as ferrous iron permease in proteoliposomes in vitro. Topology analysis indicated that EfeU is an integral cytoplasmic membrane protein exhibiting seven transmembrane helices. Two REXXE motifs within transmembrane helices of OFeT family members are implicated in iron translocation. Site-directed mutagenesis of each REGLE motif of EfeU diminished iron uptake in vivo and growth yield. In vitro the EfeU variant protein with an altered first REGLE motif was impaired in iron permeation, whereas activity of the EfeU variant with a mutation in the second motif was similar to the wild-type protein. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/16987175/A_new_ferrous_iron_uptake_transporter_EfeU__YcdN__from_Escherichia_coli_ L2 - https://doi.org/10.1111/j.1365-2958.2006.05326.x DB - PRIME DP - Unbound Medicine ER -