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Rad52 and Rad59 exhibit both overlapping and distinct functions.
DNA Repair (Amst). 2007 Jan 04; 6(1):27-37.DR

Abstract

Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

Authors+Show Affiliations

Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, New York, NY 10032-2704, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16987715

Citation

Feng, Qi, et al. "Rad52 and Rad59 Exhibit Both Overlapping and Distinct Functions." DNA Repair, vol. 6, no. 1, 2007, pp. 27-37.
Feng Q, Düring L, de Mayolo AA, et al. Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair (Amst). 2007;6(1):27-37.
Feng, Q., Düring, L., de Mayolo, A. A., Lettier, G., Lisby, M., Erdeniz, N., Mortensen, U. H., & Rothstein, R. (2007). Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair, 6(1), 27-37.
Feng Q, et al. Rad52 and Rad59 Exhibit Both Overlapping and Distinct Functions. DNA Repair (Amst). 2007 Jan 4;6(1):27-37. PubMed PMID: 16987715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions. AU - Feng,Qi, AU - Düring,Louis, AU - de Mayolo,Adriana Antúnez, AU - Lettier,Gaëlle, AU - Lisby,Michael, AU - Erdeniz,Naz, AU - Mortensen,Uffe H, AU - Rothstein,Rodney, Y1 - 2006/09/20/ PY - 2006/07/07/received PY - 2006/08/09/revised PY - 2006/08/09/accepted PY - 2006/9/22/pubmed PY - 2007/3/3/medline PY - 2006/9/22/entrez SP - 27 EP - 37 JF - DNA repair JO - DNA Repair (Amst) VL - 6 IS - 1 N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination. SN - 1568-7864 UR - https://www.unboundmedicine.com/medline/citation/16987715/Rad52_and_Rad59_exhibit_both_overlapping_and_distinct_functions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-7864(06)00274-6 DB - PRIME DP - Unbound Medicine ER -