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Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum.
Clin Vaccine Immunol. 2006 Nov; 13(11):1185-9.CV

Abstract

We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories.

Authors+Show Affiliations

Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, 23 avenue Pasteur, BP 6010, 97306 Cayenne cedex, French Guiana. pdussart@pasteur-cayenne.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16988003

Citation

Dussart, Philippe, et al. "Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum." Clinical and Vaccine Immunology : CVI, vol. 13, no. 11, 2006, pp. 1185-9.
Dussart P, Labeau B, Lagathu G, et al. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 2006;13(11):1185-9.
Dussart, P., Labeau, B., Lagathu, G., Louis, P., Nunes, M. R., Rodrigues, S. G., Storck-Herrmann, C., Cesaire, R., Morvan, J., Flamand, M., & Baril, L. (2006). Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clinical and Vaccine Immunology : CVI, 13(11), 1185-9.
Dussart P, et al. Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum. Clin Vaccine Immunol. 2006;13(11):1185-9. PubMed PMID: 16988003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. AU - Dussart,Philippe, AU - Labeau,Bhety, AU - Lagathu,Gisèle, AU - Louis,Philippe, AU - Nunes,Marcio R T, AU - Rodrigues,Sueli G, AU - Storck-Herrmann,Cécile, AU - Cesaire,Raymond, AU - Morvan,Jacques, AU - Flamand,Marie, AU - Baril,Laurence, Y1 - 2006/09/20/ PY - 2006/9/22/pubmed PY - 2007/2/8/medline PY - 2006/9/22/entrez SP - 1185 EP - 9 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 13 IS - 11 N2 - We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories. SN - 1556-6811 UR - https://www.unboundmedicine.com/medline/citation/16988003/Evaluation_of_an_enzyme_immunoassay_for_detection_of_dengue_virus_NS1_antigen_in_human_serum_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=16988003 DB - PRIME DP - Unbound Medicine ER -