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Gene expression of leptin, resistin, and adiponectin in the white adipose tissue of obese patients with non-alcoholic fatty liver disease and insulin resistance.
Obes Surg. 2006 Sep; 16(9):1118-25.OS

Abstract

BACKGROUND

Adipose tissue is an active endocrine organ that secretes a variety of metabolically important substances including adipokines. These factors affect insulin sensitivity and may represent a link between obesity, insulin resistance, type 2 diabetes (DM), and nonalcoholic fatty liver disease (NAFLD). This study uses real-time polymerase chain reaction (PCR) quantification of mRNAs encoding adiponectin, leptin, and resistin on snap-frozen samples of intra-abdominal adipose tissue of morbidly obese patients undergoing bariatric surgery.

METHODS

Morbidly obese patients undergoing bariatric surgery were studied. Patients were classified into two groups: Group A (with insulin resistance) (N=11; glucose 149.84 +/- 40.56 mg/dL; serum insulin 8.28 +/- 3.52 microU/mL), and Group B (without insulin resistance) (N=10; glucose 102.2 +/- 8.43 mg/dL; serum insulin 3.431 +/- 1.162 microU/mL).

RESULTS

Adiponectin mRNA in intra-abdominal adipose tissue and serum adiponectin levels were significantly lower in Group A compared to Group B patients (P<0.016 and P<0.03, respectively). Although serum resistin was higher in Group A than in Group B patients (P<0.005), resistin gene expression was not different between the two groups. Finally, for leptin, neither serum level nor gene expression was different between the two groups. Serum adiponectin level was the only predictor of nonalcoholic steatohepatitis (NASH) in this study (P=0.024).

CONCLUSIONS

Obese patients with insulin resistance have decreased serum adiponectin and increased serum resistin. Additionally, adiponectin gene expression is also decreased in the adipose tissue of these patients. This low level of adiponectin expression may predispose patients to the progressive form of NAFLD or NASH.

Authors+Show Affiliations

Center for Liver Diseases, Inova Fairfax Hospital, Falls Church, VA 22042, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16989692

Citation

Baranova, Ancha, et al. "Gene Expression of Leptin, Resistin, and Adiponectin in the White Adipose Tissue of Obese Patients With Non-alcoholic Fatty Liver Disease and Insulin Resistance." Obesity Surgery, vol. 16, no. 9, 2006, pp. 1118-25.
Baranova A, Gowder SJ, Schlauch K, et al. Gene expression of leptin, resistin, and adiponectin in the white adipose tissue of obese patients with non-alcoholic fatty liver disease and insulin resistance. Obes Surg. 2006;16(9):1118-25.
Baranova, A., Gowder, S. J., Schlauch, K., Elariny, H., Collantes, R., Afendy, A., Ong, J. P., Goodman, Z., Chandhoke, V., & Younossi, Z. M. (2006). Gene expression of leptin, resistin, and adiponectin in the white adipose tissue of obese patients with non-alcoholic fatty liver disease and insulin resistance. Obesity Surgery, 16(9), 1118-25.
Baranova A, et al. Gene Expression of Leptin, Resistin, and Adiponectin in the White Adipose Tissue of Obese Patients With Non-alcoholic Fatty Liver Disease and Insulin Resistance. Obes Surg. 2006;16(9):1118-25. PubMed PMID: 16989692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression of leptin, resistin, and adiponectin in the white adipose tissue of obese patients with non-alcoholic fatty liver disease and insulin resistance. AU - Baranova,Ancha, AU - Gowder,Shobha J, AU - Schlauch,Karen, AU - Elariny,Hazem, AU - Collantes,Rochelle, AU - Afendy,Arian, AU - Ong,Janus P, AU - Goodman,Zachary, AU - Chandhoke,Vikas, AU - Younossi,Zobair M, PY - 2006/9/23/pubmed PY - 2007/4/4/medline PY - 2006/9/23/entrez SP - 1118 EP - 25 JF - Obesity surgery JO - Obes Surg VL - 16 IS - 9 N2 - BACKGROUND: Adipose tissue is an active endocrine organ that secretes a variety of metabolically important substances including adipokines. These factors affect insulin sensitivity and may represent a link between obesity, insulin resistance, type 2 diabetes (DM), and nonalcoholic fatty liver disease (NAFLD). This study uses real-time polymerase chain reaction (PCR) quantification of mRNAs encoding adiponectin, leptin, and resistin on snap-frozen samples of intra-abdominal adipose tissue of morbidly obese patients undergoing bariatric surgery. METHODS: Morbidly obese patients undergoing bariatric surgery were studied. Patients were classified into two groups: Group A (with insulin resistance) (N=11; glucose 149.84 +/- 40.56 mg/dL; serum insulin 8.28 +/- 3.52 microU/mL), and Group B (without insulin resistance) (N=10; glucose 102.2 +/- 8.43 mg/dL; serum insulin 3.431 +/- 1.162 microU/mL). RESULTS: Adiponectin mRNA in intra-abdominal adipose tissue and serum adiponectin levels were significantly lower in Group A compared to Group B patients (P<0.016 and P<0.03, respectively). Although serum resistin was higher in Group A than in Group B patients (P<0.005), resistin gene expression was not different between the two groups. Finally, for leptin, neither serum level nor gene expression was different between the two groups. Serum adiponectin level was the only predictor of nonalcoholic steatohepatitis (NASH) in this study (P=0.024). CONCLUSIONS: Obese patients with insulin resistance have decreased serum adiponectin and increased serum resistin. Additionally, adiponectin gene expression is also decreased in the adipose tissue of these patients. This low level of adiponectin expression may predispose patients to the progressive form of NAFLD or NASH. SN - 0960-8923 UR - https://www.unboundmedicine.com/medline/citation/16989692/Gene_expression_of_leptin_resistin_and_adiponectin_in_the_white_adipose_tissue_of_obese_patients_with_non_alcoholic_fatty_liver_disease_and_insulin_resistance_ L2 - https://dx.doi.org/10.1381/096089206778392149 DB - PRIME DP - Unbound Medicine ER -