Tags

Type your tag names separated by a space and hit enter

Abscisic acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress.
Plant Cell Physiol. 2006 Nov; 47(11):1484-95.PC

Abstract

The histochemical and cytochemical localization of water stress-induced H(2)O(2) production in the leaves of ABA-deficient vp5 mutant and wild-type maize (Zea mays L.) plants were examined, using 3,3-diaminobenzidine and CeCl(3) staining, respectively, and the roles of endogenous ABA in the production of H(2)O(2) induced by water stress were assessed. Water stress induced by polyethylene glycol resulted in the accumulation of H(2)O(2) in mesophyll cells, bundle-sheath cells and vascular bundles of wild-type maize leaves, and the accumulation was substantially blocked in the mutant maize leaves exposed to water stress. Pre-treatments with several apoplastic H(2)O(2) manipulators abolished the majority of H(2)O(2) accumulation induced by water stress in the wild-type leaves. The subcellular localization of H(2)O(2) production was demonstrated in the cell walls, xylem vessels, chloroplasts, mitochondria and peroxisomes in the leaves of wild-type maize plants exposed to water stress, and the accumulation of H(2)O(2) induced by water stress in the cell walls and xylem vessels, but not in the chloroplasts, mitochondria and peroxisomes, was arrested in the leaves of the ABA mutant or the ABA biosynthesis inhibitor (tungstate)-pre-treated maize plants. Pre-treatments with the apoplastic H(2)O(2) manipulators also blocked the apoplastic but not the intracellular H(2)O(2) accumulation induced by water stress in the leaves of wild-type plants. These data indicate that under water stress, the apoplast is the major source of H(2)O(2) production and ABA is a key inducer of apoplastic H(2)O(2) production. These data also suggest that H(2)O(2) generated in the apoplast could not diffuse freely into subcellular compartments.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Hu X
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Zhang A
No affiliation info available
Zhang J
No affiliation info available
Jiang M
No affiliation info available

MeSH

Abscisic AcidAntioxidantsCatalaseChloroplastsDesiccationHydrogen PeroxideMitochondriaOnium CompoundsPeroxisomesPlant LeavesSuperoxide DismutaseWaterZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16990290

Citation

Hu, Xiuli, et al. "Abscisic Acid Is a Key Inducer of Hydrogen Peroxide Production in Leaves of Maize Plants Exposed to Water Stress." Plant & Cell Physiology, vol. 47, no. 11, 2006, pp. 1484-95.
Hu X, Zhang A, Zhang J, et al. Abscisic acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress. Plant Cell Physiol. 2006;47(11):1484-95.
Hu, X., Zhang, A., Zhang, J., & Jiang, M. (2006). Abscisic acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress. Plant & Cell Physiology, 47(11), 1484-95.
Hu X, et al. Abscisic Acid Is a Key Inducer of Hydrogen Peroxide Production in Leaves of Maize Plants Exposed to Water Stress. Plant Cell Physiol. 2006;47(11):1484-95. PubMed PMID: 16990290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abscisic acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress. AU - Hu,Xiuli, AU - Zhang,Aying, AU - Zhang,Jianhua, AU - Jiang,Mingyi, Y1 - 2006/09/20/ PY - 2006/9/23/pubmed PY - 2007/2/14/medline PY - 2006/9/23/entrez SP - 1484 EP - 95 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 47 IS - 11 N2 - The histochemical and cytochemical localization of water stress-induced H(2)O(2) production in the leaves of ABA-deficient vp5 mutant and wild-type maize (Zea mays L.) plants were examined, using 3,3-diaminobenzidine and CeCl(3) staining, respectively, and the roles of endogenous ABA in the production of H(2)O(2) induced by water stress were assessed. Water stress induced by polyethylene glycol resulted in the accumulation of H(2)O(2) in mesophyll cells, bundle-sheath cells and vascular bundles of wild-type maize leaves, and the accumulation was substantially blocked in the mutant maize leaves exposed to water stress. Pre-treatments with several apoplastic H(2)O(2) manipulators abolished the majority of H(2)O(2) accumulation induced by water stress in the wild-type leaves. The subcellular localization of H(2)O(2) production was demonstrated in the cell walls, xylem vessels, chloroplasts, mitochondria and peroxisomes in the leaves of wild-type maize plants exposed to water stress, and the accumulation of H(2)O(2) induced by water stress in the cell walls and xylem vessels, but not in the chloroplasts, mitochondria and peroxisomes, was arrested in the leaves of the ABA mutant or the ABA biosynthesis inhibitor (tungstate)-pre-treated maize plants. Pre-treatments with the apoplastic H(2)O(2) manipulators also blocked the apoplastic but not the intracellular H(2)O(2) accumulation induced by water stress in the leaves of wild-type plants. These data indicate that under water stress, the apoplast is the major source of H(2)O(2) production and ABA is a key inducer of apoplastic H(2)O(2) production. These data also suggest that H(2)O(2) generated in the apoplast could not diffuse freely into subcellular compartments. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/16990290/Abscisic_acid_is_a_key_inducer_of_hydrogen_peroxide_production_in_leaves_of_maize_plants_exposed_to_water_stress_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcl014 DB - PRIME DP - Unbound Medicine ER -