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A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus.
BMC Plant Biol. 2006 Sep 22; 6:20.BP

Abstract

BACKGROUND

Eucalypts are the most widely planted hardwood trees in the world occupying globally more than 18 million hectares as an important source of carbon neutral renewable energy and raw material for pulp, paper and solid wood. Quantitative Trait Loci (QTLs) in Eucalyptus have been localized on pedigree-specific RAPD or AFLP maps seriously limiting the value of such QTL mapping efforts for molecular breeding. The availability of a genus-wide genetic map with transferable microsatellite markers has become a must for the effective advancement of genomic undertakings. This report describes the development of a novel set of 230 EMBRA microsatellites, the construction of the first comprehensive microsatellite-based consensus linkage map for Eucalyptus and the consolidation of existing linkage information for other microsatellites and candidate genes mapped in other species of the genus.

RESULTS

The consensus map covers approximately 90% of the recombining genome of Eucalyptus, involves 234 mapped EMBRA loci on 11 linkage groups, an observed length of 1,568 cM and a mean distance between markers of 8.4 cM. A compilation of all microsatellite linkage information published in Eucalyptus allowed us to establish the homology among linkage groups between this consensus map and other maps published for E. globulus. Comparative mapping analyses also resulted in the linkage group assignment of other 41 microsatellites derived from other Eucalyptus species as well as candidate genes and QTLs for wood and flowering traits published in the literature. This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus.

CONCLUSION

This work represents an important step forward for Eucalyptus comparative genomics, opening stimulating perspectives for evolutionary studies and molecular breeding applications. The generalized use of an increasingly larger set of interspecific transferable markers and consensus mapping information, will allow faster and more detailed investigations of QTL synteny among species, validation of expression-QTL across variable genetic backgrounds and positioning of a growing number of candidate genes co-localized with QTLs, to be tested in association mapping experiments.

Authors+Show Affiliations

EMBRAPA Recursos Genéticos e Biotecnologia, CP 02372, 70770-970 DF Brasilia, Brazil. rosanavb@cnpaf.embrapa.brNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16995939

Citation

Brondani, Rosana P V., et al. "A Microsatellite-based Consensus Linkage Map for Species of Eucalyptus and a Novel Set of 230 Microsatellite Markers for the Genus." BMC Plant Biology, vol. 6, 2006, p. 20.
Brondani RP, Williams ER, Brondani C, et al. A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. BMC Plant Biol. 2006;6:20.
Brondani, R. P., Williams, E. R., Brondani, C., & Grattapaglia, D. (2006). A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. BMC Plant Biology, 6, 20.
Brondani RP, et al. A Microsatellite-based Consensus Linkage Map for Species of Eucalyptus and a Novel Set of 230 Microsatellite Markers for the Genus. BMC Plant Biol. 2006 Sep 22;6:20. PubMed PMID: 16995939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. AU - Brondani,Rosana P V, AU - Williams,Emlyn R, AU - Brondani,Claudio, AU - Grattapaglia,Dario, Y1 - 2006/09/22/ PY - 2006/06/19/received PY - 2006/09/22/accepted PY - 2006/9/26/pubmed PY - 2006/11/10/medline PY - 2006/9/26/entrez SP - 20 EP - 20 JF - BMC plant biology JO - BMC Plant Biol. VL - 6 N2 - BACKGROUND: Eucalypts are the most widely planted hardwood trees in the world occupying globally more than 18 million hectares as an important source of carbon neutral renewable energy and raw material for pulp, paper and solid wood. Quantitative Trait Loci (QTLs) in Eucalyptus have been localized on pedigree-specific RAPD or AFLP maps seriously limiting the value of such QTL mapping efforts for molecular breeding. The availability of a genus-wide genetic map with transferable microsatellite markers has become a must for the effective advancement of genomic undertakings. This report describes the development of a novel set of 230 EMBRA microsatellites, the construction of the first comprehensive microsatellite-based consensus linkage map for Eucalyptus and the consolidation of existing linkage information for other microsatellites and candidate genes mapped in other species of the genus. RESULTS: The consensus map covers approximately 90% of the recombining genome of Eucalyptus, involves 234 mapped EMBRA loci on 11 linkage groups, an observed length of 1,568 cM and a mean distance between markers of 8.4 cM. A compilation of all microsatellite linkage information published in Eucalyptus allowed us to establish the homology among linkage groups between this consensus map and other maps published for E. globulus. Comparative mapping analyses also resulted in the linkage group assignment of other 41 microsatellites derived from other Eucalyptus species as well as candidate genes and QTLs for wood and flowering traits published in the literature. This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus. CONCLUSION: This work represents an important step forward for Eucalyptus comparative genomics, opening stimulating perspectives for evolutionary studies and molecular breeding applications. The generalized use of an increasingly larger set of interspecific transferable markers and consensus mapping information, will allow faster and more detailed investigations of QTL synteny among species, validation of expression-QTL across variable genetic backgrounds and positioning of a growing number of candidate genes co-localized with QTLs, to be tested in association mapping experiments. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/16995939/A_microsatellite_based_consensus_linkage_map_for_species_of_Eucalyptus_and_a_novel_set_of_230_microsatellite_markers_for_the_genus_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-6-20 DB - PRIME DP - Unbound Medicine ER -