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Protective effects of soft acrylic yellow filter against blue light-induced retinal damage in rats.

Abstract

Recently, a yellow intraocular lens (IOL) was developed for the purpose of reducing potential blue light-induced retinal damage after cataract surgery. However, the effect of yellow filters on retinal protection remains to be clarified. To test the protective effects of yellow filters on blue light-induced retinal damage, a yellow and a clear soft acrylic filter were attached to the right and left eyes, respectively, of albino rats and exposed to 4.5 k lux blue fluorescent lights with peak wavelength at 420 nm (ranging 380-500 nm; short blue) or 446 nm (ranging 400-540 nm; long blue) for 6h. To assess retinal damage, the electroretinogram (ERG) was recorded at 7 days, outer nuclear layer (ONL) thickness and area were measured at 7 days, apoptosis was analyzed by TUNEL staining at 24 h, and the level of lipid peroxidation in retinas was assessed by Western dot blots using specific antibodies against 4-hydroxynonenal (4-HNE)- and carboxyethylpyrrole (CEP)-modified proteins immediately after light exposure. After short blue light exposure, a- and b-wave ERG amplitudes and the ONL thickness at 1-2.5 mm inferior and 0.5-2.5 mm superior to optic nerve head (ONH) were significantly reduced. TUNEL staining in the ONL at 0-2 mm inferior and 1-2 mm superior to the ONH, and retinal levels of 4-HNE- and CEP-modified proteins were significantly increased in the clear filter-covered eyes compared to yellow filter-covered eyes. After long blue light exposure, the only difference seen was a greater ONL thickness at 1.5 mm superior to the ONH in yellow filter-covered eye. Transmission of light through the yellow filter was 58% for short blue and 89% for long blue compared to the clear filter. The ONL area was not different between clear filter-covered and -uncovered eyes after exposure to short or long blue light. Given the results, yellow IOL material protects the retina against acute shorter wavelength blue light exposure more effectively than the clear IOL material.

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  • Authors+Show Affiliations

    ,

    Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. tanito-oph@umin.ac.jp

    ,

    Source

    Experimental eye research 83:6 2006 Dec pg 1493-504

    MeSH

    Animals
    Apoptosis
    Color
    Electroretinography
    Eye Proteins
    Filtration
    Lens, Crystalline
    Light
    Lipid Peroxidation
    Photoreceptor Cells, Vertebrate
    Radiation Injuries, Experimental
    Rats
    Rats, Sprague-Dawley
    Retina
    Retinal Degeneration
    Scattering, Radiation
    Ultraviolet Rays

    Pub Type(s)

    Evaluation Studies
    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16997296

    Citation

    Tanito, Masaki, et al. "Protective Effects of Soft Acrylic Yellow Filter Against Blue Light-induced Retinal Damage in Rats." Experimental Eye Research, vol. 83, no. 6, 2006, pp. 1493-504.
    Tanito M, Kaidzu S, Anderson RE. Protective effects of soft acrylic yellow filter against blue light-induced retinal damage in rats. Exp Eye Res. 2006;83(6):1493-504.
    Tanito, M., Kaidzu, S., & Anderson, R. E. (2006). Protective effects of soft acrylic yellow filter against blue light-induced retinal damage in rats. Experimental Eye Research, 83(6), pp. 1493-504.
    Tanito M, Kaidzu S, Anderson RE. Protective Effects of Soft Acrylic Yellow Filter Against Blue Light-induced Retinal Damage in Rats. Exp Eye Res. 2006;83(6):1493-504. PubMed PMID: 16997296.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Protective effects of soft acrylic yellow filter against blue light-induced retinal damage in rats. AU - Tanito,Masaki, AU - Kaidzu,Sachiko, AU - Anderson,Robert E, Y1 - 2006/09/25/ PY - 2006/06/02/received PY - 2006/06/29/revised PY - 2006/08/09/accepted PY - 2006/9/26/pubmed PY - 2007/2/21/medline PY - 2006/9/26/entrez SP - 1493 EP - 504 JF - Experimental eye research JO - Exp. Eye Res. VL - 83 IS - 6 N2 - Recently, a yellow intraocular lens (IOL) was developed for the purpose of reducing potential blue light-induced retinal damage after cataract surgery. However, the effect of yellow filters on retinal protection remains to be clarified. To test the protective effects of yellow filters on blue light-induced retinal damage, a yellow and a clear soft acrylic filter were attached to the right and left eyes, respectively, of albino rats and exposed to 4.5 k lux blue fluorescent lights with peak wavelength at 420 nm (ranging 380-500 nm; short blue) or 446 nm (ranging 400-540 nm; long blue) for 6h. To assess retinal damage, the electroretinogram (ERG) was recorded at 7 days, outer nuclear layer (ONL) thickness and area were measured at 7 days, apoptosis was analyzed by TUNEL staining at 24 h, and the level of lipid peroxidation in retinas was assessed by Western dot blots using specific antibodies against 4-hydroxynonenal (4-HNE)- and carboxyethylpyrrole (CEP)-modified proteins immediately after light exposure. After short blue light exposure, a- and b-wave ERG amplitudes and the ONL thickness at 1-2.5 mm inferior and 0.5-2.5 mm superior to optic nerve head (ONH) were significantly reduced. TUNEL staining in the ONL at 0-2 mm inferior and 1-2 mm superior to the ONH, and retinal levels of 4-HNE- and CEP-modified proteins were significantly increased in the clear filter-covered eyes compared to yellow filter-covered eyes. After long blue light exposure, the only difference seen was a greater ONL thickness at 1.5 mm superior to the ONH in yellow filter-covered eye. Transmission of light through the yellow filter was 58% for short blue and 89% for long blue compared to the clear filter. The ONL area was not different between clear filter-covered and -uncovered eyes after exposure to short or long blue light. Given the results, yellow IOL material protects the retina against acute shorter wavelength blue light exposure more effectively than the clear IOL material. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/16997296/Protective_effects_of_soft_acrylic_yellow_filter_against_blue_light_induced_retinal_damage_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(06)00346-0 DB - PRIME DP - Unbound Medicine ER -