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Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
Appl Environ Microbiol. 2006 Nov; 72(11):7401-5.AE

Abstract

In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.

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Authors+Show Affiliations

Klijn A
Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.
Moine D
No affiliation info available
Delley M
No affiliation info available
Mercenier A
No affiliation info available
Arigoni F
No affiliation info available
Pridmore RD
No affiliation info available

MeSH

Base SequenceBifidobacteriumEscherichia coliGenes, ReporterGenetic VectorsGlucuronidaseMolecular Sequence DataPlasmidsPromoter Regions, GeneticSequence Analysis, DNA

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16997985

Citation

Klijn, A, et al. "Construction of a Reporter Vector for the Analysis of Bifidobacterium Longum Promoters." Applied and Environmental Microbiology, vol. 72, no. 11, 2006, pp. 7401-5.
Klijn A, Moine D, Delley M, et al. Construction of a reporter vector for the analysis of Bifidobacterium longum promoters. Appl Environ Microbiol. 2006;72(11):7401-5.
Klijn, A., Moine, D., Delley, M., Mercenier, A., Arigoni, F., & Pridmore, R. D. (2006). Construction of a reporter vector for the analysis of Bifidobacterium longum promoters. Applied and Environmental Microbiology, 72(11), 7401-5.
Klijn A, et al. Construction of a Reporter Vector for the Analysis of Bifidobacterium Longum Promoters. Appl Environ Microbiol. 2006;72(11):7401-5. PubMed PMID: 16997985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a reporter vector for the analysis of Bifidobacterium longum promoters. AU - Klijn,A, AU - Moine,D, AU - Delley,M, AU - Mercenier,A, AU - Arigoni,F, AU - Pridmore,R D, Y1 - 2006/09/22/ PY - 2006/9/26/pubmed PY - 2007/1/24/medline PY - 2006/9/26/entrez SP - 7401 EP - 5 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 72 IS - 11 N2 - In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16997985/Construction_of_a_reporter_vector_for_the_analysis_of_Bifidobacterium_longum_promoters_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16997985 DB - PRIME DP - Unbound Medicine ER -