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Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain.
J Clin Microbiol. 1990 Oct; 28(10):2228-33.JC

Abstract

A collaborative study was undertaken at two institutions to assess the performance of a direct fluorescent-antibody stain for the detection of Pneumocystis carinii in respiratory specimens from patients with known or suspected human immunodeficiency virus type 1 infections. A total of 163 specimens (125 induced sputa, 37 bronchoalveolar lavage fluids, and 1 tracheal aspirate) from 124 patients were examined by using modified Giemsa (Diff-Quik; Baxter American Scientific Products, Chicago, Ill.) and direct fluorescent-antibody stains. A total of 73 specimens contained P. carinii, which was detected in 66 (92%) of the specimens by using the modified Giemsa and in 71 (97%) of the specimens by using the fluorescent-antibody stain. One bronchoalveolar lavage fluid specimen in which P. carinii was detected only with the fluorescent-antibody stain was determined to be a false-positive based on subsequent clinical evaluation of the patient. Although the overall time for processing and examining specimens stained with either stain was not significantly different for those specimens containing P. carinii, considerably less time was required for microscopic examination of those fluorescent-antibody-stained specimens lacking P. carinii.

Authors+Show Affiliations

Department of Laboratory Medicine, University of California, San Francisco 94110.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1699968

Citation

Ng, V L., et al. "Rapid Detection of Pneumocystis Carinii Using a Direct Fluorescent Monoclonal Antibody Stain." Journal of Clinical Microbiology, vol. 28, no. 10, 1990, pp. 2228-33.
Ng VL, Virani NA, Chaisson RE, et al. Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain. J Clin Microbiol. 1990;28(10):2228-33.
Ng, V. L., Virani, N. A., Chaisson, R. E., Yajko, D. M., Sphar, H. T., Cabrian, K., Rollins, N., Charache, P., Krieger, M., & Hadley, W. K. (1990). Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain. Journal of Clinical Microbiology, 28(10), 2228-33.
Ng VL, et al. Rapid Detection of Pneumocystis Carinii Using a Direct Fluorescent Monoclonal Antibody Stain. J Clin Microbiol. 1990;28(10):2228-33. PubMed PMID: 1699968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain. A1 - Ng,V L, AU - Virani,N A, AU - Chaisson,R E, AU - Yajko,D M, AU - Sphar,H T, AU - Cabrian,K, AU - Rollins,N, AU - Charache,P, AU - Krieger,M, AU - Hadley,W K, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 2228 EP - 33 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 28 IS - 10 N2 - A collaborative study was undertaken at two institutions to assess the performance of a direct fluorescent-antibody stain for the detection of Pneumocystis carinii in respiratory specimens from patients with known or suspected human immunodeficiency virus type 1 infections. A total of 163 specimens (125 induced sputa, 37 bronchoalveolar lavage fluids, and 1 tracheal aspirate) from 124 patients were examined by using modified Giemsa (Diff-Quik; Baxter American Scientific Products, Chicago, Ill.) and direct fluorescent-antibody stains. A total of 73 specimens contained P. carinii, which was detected in 66 (92%) of the specimens by using the modified Giemsa and in 71 (97%) of the specimens by using the fluorescent-antibody stain. One bronchoalveolar lavage fluid specimen in which P. carinii was detected only with the fluorescent-antibody stain was determined to be a false-positive based on subsequent clinical evaluation of the patient. Although the overall time for processing and examining specimens stained with either stain was not significantly different for those specimens containing P. carinii, considerably less time was required for microscopic examination of those fluorescent-antibody-stained specimens lacking P. carinii. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/1699968/Rapid_detection_of_Pneumocystis_carinii_using_a_direct_fluorescent_monoclonal_antibody_stain_ L2 - https://journals.asm.org/doi/10.1128/jcm.28.10.2228-2233.1990?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -