Tags

Type your tag names separated by a space and hit enter

Differential expression and regulation of progesterone receptor isoforms in rat and mouse pituitary cells and LbetaT2 gonadotropes.
J Endocrinol. 2006 Sep; 190(3):837-46.JE

Abstract

Manipulation of endogenous progesterone receptor (PR) does not produce equivalent physiological effects in mouse and rat pituitary cells. To test whether this may be due in part to difference in PR isoform expression, we examined hormonally regulated pituitary PR-A and PR-B mRNA levels using quantitative real-time PCR. The LbetaT2 mouse gonadotrope line or pituitary cells from adult, ovariectomized rats or mice were cultured with or without 0.2 nM 17beta-estradiol (E(2)) for 3 days. PR-A was the predominant form expressed for all groups. For mouse cells, E(2) led to an increase in both isoforms without a change in the A:B ratio; for rat cells, the PR-B response to E(2) was more robust resulting in a decrease in the A:B ratio. Exposure of E(2)-treated pituitary cells to 200 nM progesterone for 6 h decreased both PR-A and PR-B levels in rat cells, but had no effect on PR isoform expression in mouse cells even when exposure was extended to 12 h. The low level of PR expression found in LbetaT2 gonadotropes was unaffected by E(2), alone or with progesterone. The weak PR expression and lack of responsiveness of LbetaT2 cells cannot be explained by a male phenotype as was shown by the more than tenfold higher PR mRNA level in primary cultures of male mouse pituitary cells, which responded to E(2) stimulation with a proportional increase in PR isoforms similar to female cells. Functionally, E(2)-stimulated changes in PR mRNA isoform ratios in rat, mouse or LbetaT2 cells correlated with the degree of progesterone augmentation of GnRH-stimulated LH secretion in these models. These results are consistent with the hypothesis that robust GnRH priming and progesterone augmentation of LH secretion in the rat compared to these events in the mouse are a consequence, in part, of differences in the E(2)-modulated ratio of PR isoforms.

Authors+Show Affiliations

Division of Endocrinology, Department of Internal Medicine, School of Medicine, University of California Davis, Davis, California 95616, USA. jlturgeon@ucdavis.eduNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17003284

Citation

Turgeon, Judith L., and Dennis W. Waring. "Differential Expression and Regulation of Progesterone Receptor Isoforms in Rat and Mouse Pituitary Cells and LbetaT2 Gonadotropes." The Journal of Endocrinology, vol. 190, no. 3, 2006, pp. 837-46.
Turgeon JL, Waring DW. Differential expression and regulation of progesterone receptor isoforms in rat and mouse pituitary cells and LbetaT2 gonadotropes. J Endocrinol. 2006;190(3):837-46.
Turgeon, J. L., & Waring, D. W. (2006). Differential expression and regulation of progesterone receptor isoforms in rat and mouse pituitary cells and LbetaT2 gonadotropes. The Journal of Endocrinology, 190(3), 837-46.
Turgeon JL, Waring DW. Differential Expression and Regulation of Progesterone Receptor Isoforms in Rat and Mouse Pituitary Cells and LbetaT2 Gonadotropes. J Endocrinol. 2006;190(3):837-46. PubMed PMID: 17003284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression and regulation of progesterone receptor isoforms in rat and mouse pituitary cells and LbetaT2 gonadotropes. AU - Turgeon,Judith L, AU - Waring,Dennis W, PY - 2006/9/28/pubmed PY - 2006/12/19/medline PY - 2006/9/28/entrez SP - 837 EP - 46 JF - The Journal of endocrinology JO - J Endocrinol VL - 190 IS - 3 N2 - Manipulation of endogenous progesterone receptor (PR) does not produce equivalent physiological effects in mouse and rat pituitary cells. To test whether this may be due in part to difference in PR isoform expression, we examined hormonally regulated pituitary PR-A and PR-B mRNA levels using quantitative real-time PCR. The LbetaT2 mouse gonadotrope line or pituitary cells from adult, ovariectomized rats or mice were cultured with or without 0.2 nM 17beta-estradiol (E(2)) for 3 days. PR-A was the predominant form expressed for all groups. For mouse cells, E(2) led to an increase in both isoforms without a change in the A:B ratio; for rat cells, the PR-B response to E(2) was more robust resulting in a decrease in the A:B ratio. Exposure of E(2)-treated pituitary cells to 200 nM progesterone for 6 h decreased both PR-A and PR-B levels in rat cells, but had no effect on PR isoform expression in mouse cells even when exposure was extended to 12 h. The low level of PR expression found in LbetaT2 gonadotropes was unaffected by E(2), alone or with progesterone. The weak PR expression and lack of responsiveness of LbetaT2 cells cannot be explained by a male phenotype as was shown by the more than tenfold higher PR mRNA level in primary cultures of male mouse pituitary cells, which responded to E(2) stimulation with a proportional increase in PR isoforms similar to female cells. Functionally, E(2)-stimulated changes in PR mRNA isoform ratios in rat, mouse or LbetaT2 cells correlated with the degree of progesterone augmentation of GnRH-stimulated LH secretion in these models. These results are consistent with the hypothesis that robust GnRH priming and progesterone augmentation of LH secretion in the rat compared to these events in the mouse are a consequence, in part, of differences in the E(2)-modulated ratio of PR isoforms. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/17003284/Differential_expression_and_regulation_of_progesterone_receptor_isoforms_in_rat_and_mouse_pituitary_cells_and_LbetaT2_gonadotropes_ L2 - https://joe.bioscientifica.com/doi/10.1677/joe.1.06923 DB - PRIME DP - Unbound Medicine ER -