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Assessment of oxidative stress in chronic pancreatitis patients.
World J Gastroenterol. 2006 Sep 21; 12(35):5705-10.WJ

Abstract

AIM

To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control subjects, by using several different analytical techniques.

METHODS

Thirty-five CP patients and 35 healthy control subjects were investigated prospectively with respect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood.

RESULTS

The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxidant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients.

CONCLUSION

Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing antioxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxidant therapy.

Authors+Show Affiliations

Department of Gastroenterology, Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen 6500 HB, The Netherlands. m.verlaan@mdl.umcn.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17007026

Citation

Verlaan, Mariette, et al. "Assessment of Oxidative Stress in Chronic Pancreatitis Patients." World Journal of Gastroenterology, vol. 12, no. 35, 2006, pp. 5705-10.
Verlaan M, Roelofs HM, van-Schaik A, et al. Assessment of oxidative stress in chronic pancreatitis patients. World J Gastroenterol. 2006;12(35):5705-10.
Verlaan, M., Roelofs, H. M., van-Schaik, A., Wanten, G. J., Jansen, J. B., Peters, W. H., & Drenth, J. P. (2006). Assessment of oxidative stress in chronic pancreatitis patients. World Journal of Gastroenterology, 12(35), 5705-10.
Verlaan M, et al. Assessment of Oxidative Stress in Chronic Pancreatitis Patients. World J Gastroenterol. 2006 Sep 21;12(35):5705-10. PubMed PMID: 17007026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of oxidative stress in chronic pancreatitis patients. AU - Verlaan,Mariette, AU - Roelofs,Hennie M J, AU - van-Schaik,Annie, AU - Wanten,Geert J A, AU - Jansen,Jan B M J, AU - Peters,Wilbert H M, AU - Drenth,Joost P H, PY - 2006/9/29/pubmed PY - 2006/12/9/medline PY - 2006/9/29/entrez SP - 5705 EP - 10 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 12 IS - 35 N2 - AIM: To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control subjects, by using several different analytical techniques. METHODS: Thirty-five CP patients and 35 healthy control subjects were investigated prospectively with respect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood. RESULTS: The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxidant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients. CONCLUSION: Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing antioxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxidant therapy. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/17007026/Assessment_of_oxidative_stress_in_chronic_pancreatitis_patients_ L2 - http://www.wjgnet.com/1007-9327/full/v12/i35/5705.htm DB - PRIME DP - Unbound Medicine ER -