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Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat.
Theor Appl Genet. 2006 Dec; 114(1):21-30.TA

Abstract

Wheat expressed sequence tags (wESTs) were identified in a genomic interval predicted to span the Lr34/Yr18 slow rusting region on chromosome 7DS and that corresponded to genes located in the syntenic region of rice chromosome 6 (between 2.02 and 2.38 Mb). A subset of the wESTs was also used to identify corresponding bacterial artificial chromosome (BAC) clones from the diploid D genome of wheat (Aegilops tauschii). Conservation and deviation of micro-colinearity within blocks of genes were found in the D genome BACs relative to the orthologous sequences in rice. Extensive RFLP analysis using the wEST derived clones as probes on a panel of wheat genetic stocks with or without Lr34/Yr18 revealed monomorphic patterns as the norm in this region of the wheat genome. A similar pattern was observed with single nucleotide polymorphism analysis on a subset of the wEST derived clones and subclones from corresponding D genome BACs. One exception was a wEST derived clone that produced a consistent RFLP pattern that distinguished the Lr34/Yr18 genetic stocks and well-established cultivars known either to possess or lack Lr34/Yr18. Conversion of the RFLP to a codominant sequence tagged site (csLV34) revealed a bi-allelic locus, where a variant size of 79 bp insertion in an intron sequence was associated with lines or cultivars that lacked Lr34/Yr18. This association with Lr34/Yr18 was validated in wheat cultivars from diverse backgrounds. Genetic linkage between csLV34 and Lr34/Yr18 was estimated at 0.4 cM.

Authors+Show Affiliations

Lagudah ES
CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia. evans.lagudah@csiro.au
McFadden H
No affiliation info available
Singh RP
No affiliation info available
Huerta-Espino J
No affiliation info available
Bariana HS
No affiliation info available
Spielmeyer W
No affiliation info available

MeSH

Chromosome MappingChromosomes, Artificial, BacterialChromosomes, PlantExpressed Sequence TagsGenes, PlantGenetic MarkersGenome, PlantPhenotypePlant DiseasesPolymorphism, Restriction Fragment LengthTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17008991

Citation

Lagudah, E S., et al. "Molecular Genetic Characterization of the Lr34/Yr18 Slow Rusting Resistance Gene Region in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 114, no. 1, 2006, pp. 21-30.
Lagudah ES, McFadden H, Singh RP, et al. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet. 2006;114(1):21-30.
Lagudah, E. S., McFadden, H., Singh, R. P., Huerta-Espino, J., Bariana, H. S., & Spielmeyer, W. (2006). Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 114(1), 21-30.
Lagudah ES, et al. Molecular Genetic Characterization of the Lr34/Yr18 Slow Rusting Resistance Gene Region in Wheat. Theor Appl Genet. 2006;114(1):21-30. PubMed PMID: 17008991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. AU - Lagudah,E S, AU - McFadden,H, AU - Singh,R P, AU - Huerta-Espino,J, AU - Bariana,H S, AU - Spielmeyer,W, Y1 - 2006/09/29/ PY - 2006/06/29/received PY - 2006/08/31/accepted PY - 2006/9/30/pubmed PY - 2007/2/14/medline PY - 2006/9/30/entrez SP - 21 EP - 30 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 114 IS - 1 N2 - Wheat expressed sequence tags (wESTs) were identified in a genomic interval predicted to span the Lr34/Yr18 slow rusting region on chromosome 7DS and that corresponded to genes located in the syntenic region of rice chromosome 6 (between 2.02 and 2.38 Mb). A subset of the wESTs was also used to identify corresponding bacterial artificial chromosome (BAC) clones from the diploid D genome of wheat (Aegilops tauschii). Conservation and deviation of micro-colinearity within blocks of genes were found in the D genome BACs relative to the orthologous sequences in rice. Extensive RFLP analysis using the wEST derived clones as probes on a panel of wheat genetic stocks with or without Lr34/Yr18 revealed monomorphic patterns as the norm in this region of the wheat genome. A similar pattern was observed with single nucleotide polymorphism analysis on a subset of the wEST derived clones and subclones from corresponding D genome BACs. One exception was a wEST derived clone that produced a consistent RFLP pattern that distinguished the Lr34/Yr18 genetic stocks and well-established cultivars known either to possess or lack Lr34/Yr18. Conversion of the RFLP to a codominant sequence tagged site (csLV34) revealed a bi-allelic locus, where a variant size of 79 bp insertion in an intron sequence was associated with lines or cultivars that lacked Lr34/Yr18. This association with Lr34/Yr18 was validated in wheat cultivars from diverse backgrounds. Genetic linkage between csLV34 and Lr34/Yr18 was estimated at 0.4 cM. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/17008991/Molecular_genetic_characterization_of_the_Lr34/Yr18_slow_rusting_resistance_gene_region_in_wheat_ L2 - https://dx.doi.org/10.1007/s00122-006-0406-z DB - PRIME DP - Unbound Medicine ER -