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Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography-mass spectrometry-olfactometry.
Anal Bioanal Chem. 2006 Nov; 386(6):1791-802.AB

Abstract

Volatile organic compounds (VOCs) and odors in cattle rumen gas have been characterized by in-vivo headspace sampling by solid-phase microextraction (SPME) and analysis by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A novel device enabling headspace SPME (HS-SPME) sampling through a cannula was designed, refined, and used to collect rumen gas samples from steers. A Carboxen-polydimethylsiloxane (PDMS) fiber (85 microm) was used for SPME sampling. Fifty VOCs from ten chemical groups were identified in the rumen headspace. The VOCs identified had a wide range of molecular weight (MW) (34 to 184), boiling point (-63.3 to 292 degrees C), vapor pressure (1.05x10(-5) to 1.17x10(2) Pa), and water solubility (0.66 to 1x10(6) mg L-1). Twenty-two of the compounds have a published odor detection thresholds (ODT) of less than 1 ppm. More than half of the compounds identified are reactive and have an estimated atmospheric lifetime of <24 h. The amounts of VFAs, sulfide compounds, phenolic compounds, and skatole, and the odor intensity of VFAs and sulfide compounds in the rumen gas were all higher after feeding than before feeding. These results indicate that rumen gases can be an important potential source of aerial emissions of reactive VOCs and odor. In-vivo sampling by SPME then GC-MS-O analysis can be a useful tool for qualitative characterization of rumen gases, digestion, and its relationship to odor and VOC formation.

Authors+Show Affiliations

Cai L
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
Koziel JA
No affiliation info available
Davis J
No affiliation info available
Lo YC
No affiliation info available
Xin H
No affiliation info available

MeSH

Animal FeedAnimalsCattleGas Chromatography-Mass SpectrometryOdorantsOrganic ChemicalsRumenSolid Phase MicroextractionVolatilization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17009001

Citation

Cai, Lingshuang, et al. "Characterization of Volatile Organic Compounds and Odors By In-vivo Sampling of Beef Cattle Rumen Gas, By Solid-phase Microextraction, and Gas Chromatography-mass Spectrometry-olfactometry." Analytical and Bioanalytical Chemistry, vol. 386, no. 6, 2006, pp. 1791-802.
Cai L, Koziel JA, Davis J, et al. Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography-mass spectrometry-olfactometry. Anal Bioanal Chem. 2006;386(6):1791-802.
Cai, L., Koziel, J. A., Davis, J., Lo, Y. C., & Xin, H. (2006). Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography-mass spectrometry-olfactometry. Analytical and Bioanalytical Chemistry, 386(6), 1791-802.
Cai L, et al. Characterization of Volatile Organic Compounds and Odors By In-vivo Sampling of Beef Cattle Rumen Gas, By Solid-phase Microextraction, and Gas Chromatography-mass Spectrometry-olfactometry. Anal Bioanal Chem. 2006;386(6):1791-802. PubMed PMID: 17009001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography-mass spectrometry-olfactometry. AU - Cai,Lingshuang, AU - Koziel,Jacek A, AU - Davis,Jeremiah, AU - Lo,Yin-Cheung, AU - Xin,Hongwei, Y1 - 2006/09/29/ PY - 2006/07/24/received PY - 2006/08/22/accepted PY - 2006/08/21/revised PY - 2006/9/30/pubmed PY - 2007/2/16/medline PY - 2006/9/30/entrez SP - 1791 EP - 802 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 386 IS - 6 N2 - Volatile organic compounds (VOCs) and odors in cattle rumen gas have been characterized by in-vivo headspace sampling by solid-phase microextraction (SPME) and analysis by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A novel device enabling headspace SPME (HS-SPME) sampling through a cannula was designed, refined, and used to collect rumen gas samples from steers. A Carboxen-polydimethylsiloxane (PDMS) fiber (85 microm) was used for SPME sampling. Fifty VOCs from ten chemical groups were identified in the rumen headspace. The VOCs identified had a wide range of molecular weight (MW) (34 to 184), boiling point (-63.3 to 292 degrees C), vapor pressure (1.05x10(-5) to 1.17x10(2) Pa), and water solubility (0.66 to 1x10(6) mg L-1). Twenty-two of the compounds have a published odor detection thresholds (ODT) of less than 1 ppm. More than half of the compounds identified are reactive and have an estimated atmospheric lifetime of <24 h. The amounts of VFAs, sulfide compounds, phenolic compounds, and skatole, and the odor intensity of VFAs and sulfide compounds in the rumen gas were all higher after feeding than before feeding. These results indicate that rumen gases can be an important potential source of aerial emissions of reactive VOCs and odor. In-vivo sampling by SPME then GC-MS-O analysis can be a useful tool for qualitative characterization of rumen gases, digestion, and its relationship to odor and VOC formation. SN - 1618-2642 UR - https://www.unboundmedicine.com/medline/citation/17009001/Characterization_of_volatile_organic_compounds_and_odors_by_in_vivo_sampling_of_beef_cattle_rumen_gas_by_solid_phase_microextraction_and_gas_chromatography_mass_spectrometry_olfactometry_ L2 - https://dx.doi.org/10.1007/s00216-006-0799-1 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓14]

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