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Effect of recombinant human leukocyte, fibroblast, and immune interferons on expression of class I and II major histocompatibility complex and invariant chain in early passage human melanoma cells.
Cancer Res. 1990 Dec 01; 50(23):7422-9.CR

Abstract

Twenty-five early-passage (less than or equal to 8) melanoma cell lines, isolated from ten patients with metastatic melanoma, were analyzed by a combination of serological, immunochemical, and molecular methods for mRNA levels, synthesis, and surface expression of MHC class I and class II antigens prior to and following exposure to recombinant human leukocyte (IFN-alpha A), fibroblast (IFN-beta), and immune (IFN-gamma) interferon. All the cell lines expressed variable levels of HLA class I gene products that were up-regulated to different extents upon exposure to specific interferons (IFNs). HLA class II antigens were expressed in 22 of the 25 melanoma lines and IFN-gamma increased the levels of class II mRNA, protein synthesis, and surface expression in all cultures displaying baseline expression. A significant up-regulation of class II antigen expression by IFN-alpha or -beta, associated with higher levels of class II transcripts and enhanced synthesis, was found only in two early-passage human melanoma cell lines. In three lesions from the same patient which did not constitutively express class II antigens, no expression of these glycoproteins could be induced with any of the IFNs. These results indicate that IFN-gamma does not act as a de novo inducer of class II antigen expression in early-passage human melanoma cell lines. This hypothesis is further supported by analysis of class II-associated invariant chain (Ii) expression, which is expressed and induced by IFNs in a manner similar to that of class II antigens. The present study also indicates that early-passage metastatic melanoma lesions from the same patient are heterogeneous in their de novo expression of major histocompatibility antigens and in their modulation by IFNs.

Authors+Show Affiliations

Experimental Oncology Department, Regina Elena Cancer Institute, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1701342

Citation

Nisticò, P, et al. "Effect of Recombinant Human Leukocyte, Fibroblast, and Immune Interferons On Expression of Class I and II Major Histocompatibility Complex and Invariant Chain in Early Passage Human Melanoma Cells." Cancer Research, vol. 50, no. 23, 1990, pp. 7422-9.
Nisticò P, Tecce R, Giacomini P, et al. Effect of recombinant human leukocyte, fibroblast, and immune interferons on expression of class I and II major histocompatibility complex and invariant chain in early passage human melanoma cells. Cancer Res. 1990;50(23):7422-9.
Nisticò, P., Tecce, R., Giacomini, P., Cavallari, A., D'Agnano, I., Fisher, P. B., & Natali, P. G. (1990). Effect of recombinant human leukocyte, fibroblast, and immune interferons on expression of class I and II major histocompatibility complex and invariant chain in early passage human melanoma cells. Cancer Research, 50(23), 7422-9.
Nisticò P, et al. Effect of Recombinant Human Leukocyte, Fibroblast, and Immune Interferons On Expression of Class I and II Major Histocompatibility Complex and Invariant Chain in Early Passage Human Melanoma Cells. Cancer Res. 1990 Dec 1;50(23):7422-9. PubMed PMID: 1701342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of recombinant human leukocyte, fibroblast, and immune interferons on expression of class I and II major histocompatibility complex and invariant chain in early passage human melanoma cells. AU - Nisticò,P, AU - Tecce,R, AU - Giacomini,P, AU - Cavallari,A, AU - D'Agnano,I, AU - Fisher,P B, AU - Natali,P G, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 7422 EP - 9 JF - Cancer research JO - Cancer Res VL - 50 IS - 23 N2 - Twenty-five early-passage (less than or equal to 8) melanoma cell lines, isolated from ten patients with metastatic melanoma, were analyzed by a combination of serological, immunochemical, and molecular methods for mRNA levels, synthesis, and surface expression of MHC class I and class II antigens prior to and following exposure to recombinant human leukocyte (IFN-alpha A), fibroblast (IFN-beta), and immune (IFN-gamma) interferon. All the cell lines expressed variable levels of HLA class I gene products that were up-regulated to different extents upon exposure to specific interferons (IFNs). HLA class II antigens were expressed in 22 of the 25 melanoma lines and IFN-gamma increased the levels of class II mRNA, protein synthesis, and surface expression in all cultures displaying baseline expression. A significant up-regulation of class II antigen expression by IFN-alpha or -beta, associated with higher levels of class II transcripts and enhanced synthesis, was found only in two early-passage human melanoma cell lines. In three lesions from the same patient which did not constitutively express class II antigens, no expression of these glycoproteins could be induced with any of the IFNs. These results indicate that IFN-gamma does not act as a de novo inducer of class II antigen expression in early-passage human melanoma cell lines. This hypothesis is further supported by analysis of class II-associated invariant chain (Ii) expression, which is expressed and induced by IFNs in a manner similar to that of class II antigens. The present study also indicates that early-passage metastatic melanoma lesions from the same patient are heterogeneous in their de novo expression of major histocompatibility antigens and in their modulation by IFNs. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/1701342/Effect_of_recombinant_human_leukocyte_fibroblast_and_immune_interferons_on_expression_of_class_I_and_II_major_histocompatibility_complex_and_invariant_chain_in_early_passage_human_melanoma_cells_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=1701342 DB - PRIME DP - Unbound Medicine ER -