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Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds.
Wound Repair Regen 2006 Sep-Oct; 14(5):573-80WR

Abstract

Fresh platelet concentrates are used in many centers to treat recalcitrant wounds. To extend the therapeutic shelf-life of platelets, we analyzed the wound-healing effects of fresh-frozen and freeze-dried (FD) platelet-rich plasma (PRP) using a diabetic mouse model. Db/db mice with 1.0 cm2 dorsal excisional wounds (n = 15/group) were treated with a single application of FD PRP (1.2 x 10(6) platelets/microL) with or without a stabilization solution, and compared with wounds treated with fresh-frozen, sonicated PRP, and untreated wounds. Granulation tissue area, thickness, and wound size were analyzed 9 days posttreatment. Immunostained sections were quantified for vascularity and proliferation using antiplatelet endothelial cell adhesion molecule I and antiproliferating cell nuclear antigen antibodies. The results showed that all PRP preparations increased granulation tissue formation as assessed by surface coverage, thickness, and angiogenic response, when compared with untreated wounds. In addition, wounds treated with FD PRP, and biochemically stabilized FD PRP, exhibited higher proliferative levels. The possibility to deliver growth factors using platelets, and the potential to extend the shelf-life of platelet concentrates makes freeze-drying methods particularly suitable for enhanced wound care.

Authors+Show Affiliations

Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17014669

Citation

Pietramaggiori, Giorgio, et al. "Freeze-dried Platelet-rich Plasma Shows Beneficial Healing Properties in Chronic Wounds." Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, vol. 14, no. 5, 2006, pp. 573-80.
Pietramaggiori G, Kaipainen A, Czeczuga JM, et al. Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair Regen. 2006;14(5):573-80.
Pietramaggiori, G., Kaipainen, A., Czeczuga, J. M., Wagner, C. T., & Orgill, D. P. (2006). Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, 14(5), pp. 573-80.
Pietramaggiori G, et al. Freeze-dried Platelet-rich Plasma Shows Beneficial Healing Properties in Chronic Wounds. Wound Repair Regen. 2006;14(5):573-80. PubMed PMID: 17014669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. AU - Pietramaggiori,Giorgio, AU - Kaipainen,Arja, AU - Czeczuga,Joshua M, AU - Wagner,Christopher T, AU - Orgill,Dennis P, PY - 2006/10/4/pubmed PY - 2007/2/9/medline PY - 2006/10/4/entrez SP - 573 EP - 80 JF - Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society JO - Wound Repair Regen VL - 14 IS - 5 N2 - Fresh platelet concentrates are used in many centers to treat recalcitrant wounds. To extend the therapeutic shelf-life of platelets, we analyzed the wound-healing effects of fresh-frozen and freeze-dried (FD) platelet-rich plasma (PRP) using a diabetic mouse model. Db/db mice with 1.0 cm2 dorsal excisional wounds (n = 15/group) were treated with a single application of FD PRP (1.2 x 10(6) platelets/microL) with or without a stabilization solution, and compared with wounds treated with fresh-frozen, sonicated PRP, and untreated wounds. Granulation tissue area, thickness, and wound size were analyzed 9 days posttreatment. Immunostained sections were quantified for vascularity and proliferation using antiplatelet endothelial cell adhesion molecule I and antiproliferating cell nuclear antigen antibodies. The results showed that all PRP preparations increased granulation tissue formation as assessed by surface coverage, thickness, and angiogenic response, when compared with untreated wounds. In addition, wounds treated with FD PRP, and biochemically stabilized FD PRP, exhibited higher proliferative levels. The possibility to deliver growth factors using platelets, and the potential to extend the shelf-life of platelet concentrates makes freeze-drying methods particularly suitable for enhanced wound care. SN - 1067-1927 UR - https://www.unboundmedicine.com/medline/citation/17014669/Freeze_dried_platelet_rich_plasma_shows_beneficial_healing_properties_in_chronic_wounds_ L2 - https://doi.org/10.1111/j.1743-6109.2006.00164.x DB - PRIME DP - Unbound Medicine ER -