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Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus?
Arthritis Res Ther. 2006; 8(6):R156.AR

Abstract

Apoptotic cells are thought to play an essential role in the pathogenesis of systemic lupus erythematosus (SLE). We hypothesise that delayed or altered clearance of apoptotic cells after UV irradiation will lead to inflammation in the skin of SLE patients. Fifteen SLE patients and 13 controls were irradiated with two minimal erythemal doses (MEDs) of ultraviolet B light (UVB). Subsequently, skin biopsies were analysed (immuno)histologically, over 10 days, for numbers of apoptotic cells, T cells, macrophages, and deposition of immunoglobulin and complement. Additionally, to compare results with cutaneous lesions of SLE patients, 20 biopsies of lupus erythematosus (LE) skin lesions were analysed morphologically for apoptotic cells and infiltrate. Clearance rate of apoptotic cells after irradiation did not differ between patients and controls. Influx of macrophages in dermal and epidermal layers was significantly increased in patients compared with controls. Five out of 15 patients developed a dermal infiltrate that was associated with increased epidermal influx of T cells and macrophages but not with numbers of apoptotic cells or epidermal deposition of immunoglobulins. Macrophages were ingesting multiple apoptotic bodies. Inflammatory lesions in these patients were localised near accumulations of apoptotic keratinocytes similar as was seen in the majority of LE skin lesions. In vivo clearance rate of apoptotic cells is comparable between SLE patients and controls. However, the presence of inflammatory lesions in the vicinity of apoptotic cells, as observed both in UVB-induced and in LE skin lesions in SLE patients, suggests that these lesions result from an inflammatory clearance of apoptotic cells.

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Authors+Show Affiliations

Reefman E
Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, PO Box 30,001, 9700 RB Groningen, The Netherlands. e.reefman@med.umcg.nl
de Jong MC
No affiliation info available
Kuiper H
No affiliation info available
Jonkman MF
No affiliation info available
Limburg PC
No affiliation info available
Kallenberg CG
No affiliation info available
Bijl M
No affiliation info available

MeSH

AdultAgedAntibodies, AntinuclearApoptosisFemaleHumansImmunohistochemistryIn Situ Nick-End LabelingInflammationKeratinocytesLupus Erythematosus, SystemicMacrophagesMaleMiddle AgedRadiation InjuriesSkinSkin DiseasesT-LymphocytesUltraviolet Rays

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17014704

Citation

Reefman, Esther, et al. "Is Disturbed Clearance of Apoptotic Keratinocytes Responsible for UVB-induced Inflammatory Skin Lesions in Systemic Lupus Erythematosus?" Arthritis Research & Therapy, vol. 8, no. 6, 2006, pp. R156.
Reefman E, de Jong MC, Kuiper H, et al. Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus? Arthritis Res Ther. 2006;8(6):R156.
Reefman, E., de Jong, M. C., Kuiper, H., Jonkman, M. F., Limburg, P. C., Kallenberg, C. G., & Bijl, M. (2006). Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus? Arthritis Research & Therapy, 8(6), R156.
Reefman E, et al. Is Disturbed Clearance of Apoptotic Keratinocytes Responsible for UVB-induced Inflammatory Skin Lesions in Systemic Lupus Erythematosus. Arthritis Res Ther. 2006;8(6):R156. PubMed PMID: 17014704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus? AU - Reefman,Esther, AU - de Jong,Marcelus C J M, AU - Kuiper,Hilde, AU - Jonkman,Marcel F, AU - Limburg,Pieter C, AU - Kallenberg,Cees G M, AU - Bijl,Marc, PY - 2006/08/31/received PY - 2006/10/02/accepted PY - 2006/10/4/pubmed PY - 2007/2/24/medline PY - 2006/10/4/entrez SP - R156 EP - R156 JF - Arthritis research & therapy JO - Arthritis Res Ther VL - 8 IS - 6 N2 - Apoptotic cells are thought to play an essential role in the pathogenesis of systemic lupus erythematosus (SLE). We hypothesise that delayed or altered clearance of apoptotic cells after UV irradiation will lead to inflammation in the skin of SLE patients. Fifteen SLE patients and 13 controls were irradiated with two minimal erythemal doses (MEDs) of ultraviolet B light (UVB). Subsequently, skin biopsies were analysed (immuno)histologically, over 10 days, for numbers of apoptotic cells, T cells, macrophages, and deposition of immunoglobulin and complement. Additionally, to compare results with cutaneous lesions of SLE patients, 20 biopsies of lupus erythematosus (LE) skin lesions were analysed morphologically for apoptotic cells and infiltrate. Clearance rate of apoptotic cells after irradiation did not differ between patients and controls. Influx of macrophages in dermal and epidermal layers was significantly increased in patients compared with controls. Five out of 15 patients developed a dermal infiltrate that was associated with increased epidermal influx of T cells and macrophages but not with numbers of apoptotic cells or epidermal deposition of immunoglobulins. Macrophages were ingesting multiple apoptotic bodies. Inflammatory lesions in these patients were localised near accumulations of apoptotic keratinocytes similar as was seen in the majority of LE skin lesions. In vivo clearance rate of apoptotic cells is comparable between SLE patients and controls. However, the presence of inflammatory lesions in the vicinity of apoptotic cells, as observed both in UVB-induced and in LE skin lesions in SLE patients, suggests that these lesions result from an inflammatory clearance of apoptotic cells. SN - 1478-6362 UR - https://www.unboundmedicine.com/medline/citation/17014704/Is_disturbed_clearance_of_apoptotic_keratinocytes_responsible_for_UVB_induced_inflammatory_skin_lesions_in_systemic_lupus_erythematosus L2 - https://arthritis-research.biomedcentral.com/articles/10.1186/ar2051 DB - PRIME DP - Unbound Medicine ER -