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Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes.
J Clin Periodontol 2006; 33(11):837-45JC

Abstract

AIM

To determine the concentration of naturally available biologic mediators in autologous platelet concentrates and their correlation with periodontal regeneration outcomes.

MATERIAL AND METHODS

In 25 patients with two intra-bony defects each, an autologous platelet concentrate (APC) was prepared by a laboratory thrombocyte apheresis technique pre-operatively. Both defects were treated using a bioresorbable guided tissue regeneration-membrane in combination with tricalciumphosphate (TCP). In the test defect, APC was additionally applied. In the APC, platelets were counted and the levels of growth factors and cytokines were determined by ELISA. Correlations between the platelet counts or the growth factor/cytokine levels and the potential clinical and radiographic regeneration outcomes due to APC were calculated after 3, 6, and 12 months.

RESULTS

The APC contained 2.2 x 10(6) platelets/mul, which was 7.9 times more than in the venous blood. Transforming growth factor-beta1 (TGF-beta1), insulin-like growth factor-I (IGF-I), platelet-derived growth factor-AB (PDGF-AB), PDGF-BB, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were found in the APC, whereas interleukin-1beta (IL-1beta), IL-6, tumor necrosis factor alpha (TNFalpha), IL-4, and IL-10 were not detectable. The regression analysis showed a weak correlation between the platelet counts or the growth factor levels and the clinical and radiographic regeneration outcomes (r2<or=0.4).

CONCLUSION

Autologous platelet concentrate contains relatively high concentrations of PDGF-AB, PDGF-BB, TGF-beta1, and IGF-I, but their potential influence on periodontal regeneration remains unclear.

Authors+Show Affiliations

Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany. michael.christgau@klinik.uni-regensburg.de

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17018133

Citation

Christgau, M, et al. "Growth Factors and Cytokines in Autologous Platelet Concentrate and Their Correlation to Periodontal Regeneration Outcomes." Journal of Clinical Periodontology, vol. 33, no. 11, 2006, pp. 837-45.
Christgau M, Moder D, Hiller KA, et al. Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. J Clin Periodontol. 2006;33(11):837-45.
Christgau, M., Moder, D., Hiller, K. A., Dada, A., Schmitz, G., & Schmalz, G. (2006). Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. Journal of Clinical Periodontology, 33(11), pp. 837-45.
Christgau M, et al. Growth Factors and Cytokines in Autologous Platelet Concentrate and Their Correlation to Periodontal Regeneration Outcomes. J Clin Periodontol. 2006;33(11):837-45. PubMed PMID: 17018133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. AU - Christgau,M, AU - Moder,D, AU - Hiller,K-A, AU - Dada,A, AU - Schmitz,G, AU - Schmalz,G, PY - 2006/10/5/pubmed PY - 2006/12/21/medline PY - 2006/10/5/entrez SP - 837 EP - 45 JF - Journal of clinical periodontology JO - J. Clin. Periodontol. VL - 33 IS - 11 N2 - AIM: To determine the concentration of naturally available biologic mediators in autologous platelet concentrates and their correlation with periodontal regeneration outcomes. MATERIAL AND METHODS: In 25 patients with two intra-bony defects each, an autologous platelet concentrate (APC) was prepared by a laboratory thrombocyte apheresis technique pre-operatively. Both defects were treated using a bioresorbable guided tissue regeneration-membrane in combination with tricalciumphosphate (TCP). In the test defect, APC was additionally applied. In the APC, platelets were counted and the levels of growth factors and cytokines were determined by ELISA. Correlations between the platelet counts or the growth factor/cytokine levels and the potential clinical and radiographic regeneration outcomes due to APC were calculated after 3, 6, and 12 months. RESULTS: The APC contained 2.2 x 10(6) platelets/mul, which was 7.9 times more than in the venous blood. Transforming growth factor-beta1 (TGF-beta1), insulin-like growth factor-I (IGF-I), platelet-derived growth factor-AB (PDGF-AB), PDGF-BB, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were found in the APC, whereas interleukin-1beta (IL-1beta), IL-6, tumor necrosis factor alpha (TNFalpha), IL-4, and IL-10 were not detectable. The regression analysis showed a weak correlation between the platelet counts or the growth factor levels and the clinical and radiographic regeneration outcomes (r2<or=0.4). CONCLUSION: Autologous platelet concentrate contains relatively high concentrations of PDGF-AB, PDGF-BB, TGF-beta1, and IGF-I, but their potential influence on periodontal regeneration remains unclear. SN - 0303-6979 UR - https://www.unboundmedicine.com/medline/citation/17018133/Growth_factors_and_cytokines_in_autologous_platelet_concentrate_and_their_correlation_to_periodontal_regeneration_outcomes_ L2 - https://doi.org/10.1111/j.1600-051X.2006.00991.x DB - PRIME DP - Unbound Medicine ER -