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DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice.
Virology. 2007 Feb 20; 358(2):413-23.V

Abstract

We analyzed four DNA vaccines based on DENV-2 NS1: pcENS1, encoding the C-terminal from E protein plus the NS1 region; pcENS1ANC, similar to pcENS1 plus the N-terminal sequence from NS2a (ANC); pcTPANS1, coding the t-PA signal sequence fused to NS1; and pcTPANS1ANC, similar to pcTPANS1 plus the ANC sequence. The NS1 was detected in lysates and culture supernatants from pcTPANS1-, pcENS1- and pcENS1ANC-transfected cells and not in cells with pcTPANS1ANC. Only the pcENS1ANC leads the expression of NS1 in plasma membrane, confirming the importance of ANC sequence for targeting NS1 to cell surface. High levels of antibodies recognizing conformational epitopes of NS1 were induced in mice immunized with pcTPANS1 and pcENS1, while only few pcENS1ANC-inoculated animals presented detectable anti-NS1 IgG. Protection against DENV-2 was verified in pcTPANS1- and pcENS1-immunized mice, although the plasmid pcTPANS1 induced slight higher protective immunity. These plasmids seem to activate distinct patterns of the immune system.

Authors+Show Affiliations

Laboratory of Immunopathology, Department of Bichemistry and Molecular Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil 4365, Pav. Leonidas Deane, s. 204, Rio de Janeiro, RJ, CEP 21040-900, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17020777

Citation

Costa, S M., et al. "DNA Vaccines Against Dengue Virus Based On the Ns1 Gene: the Influence of Different Signal Sequences On the Protein Expression and Its Correlation to the Immune Response Elicited in Mice." Virology, vol. 358, no. 2, 2007, pp. 413-23.
Costa SM, Azevedo AS, Paes MV, et al. DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice. Virology. 2007;358(2):413-23.
Costa, S. M., Azevedo, A. S., Paes, M. V., Sarges, F. S., Freire, M. S., & Alves, A. M. (2007). DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice. Virology, 358(2), 413-23.
Costa SM, et al. DNA Vaccines Against Dengue Virus Based On the Ns1 Gene: the Influence of Different Signal Sequences On the Protein Expression and Its Correlation to the Immune Response Elicited in Mice. Virology. 2007 Feb 20;358(2):413-23. PubMed PMID: 17020777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice. AU - Costa,S M, AU - Azevedo,A S, AU - Paes,M V, AU - Sarges,F S, AU - Freire,M S, AU - Alves,A M B, Y1 - 2006/10/03/ PY - 2006/07/17/received PY - 2006/08/21/revised PY - 2006/08/30/accepted PY - 2006/10/6/pubmed PY - 2007/4/6/medline PY - 2006/10/6/entrez SP - 413 EP - 23 JF - Virology JO - Virology VL - 358 IS - 2 N2 - We analyzed four DNA vaccines based on DENV-2 NS1: pcENS1, encoding the C-terminal from E protein plus the NS1 region; pcENS1ANC, similar to pcENS1 plus the N-terminal sequence from NS2a (ANC); pcTPANS1, coding the t-PA signal sequence fused to NS1; and pcTPANS1ANC, similar to pcTPANS1 plus the ANC sequence. The NS1 was detected in lysates and culture supernatants from pcTPANS1-, pcENS1- and pcENS1ANC-transfected cells and not in cells with pcTPANS1ANC. Only the pcENS1ANC leads the expression of NS1 in plasma membrane, confirming the importance of ANC sequence for targeting NS1 to cell surface. High levels of antibodies recognizing conformational epitopes of NS1 were induced in mice immunized with pcTPANS1 and pcENS1, while only few pcENS1ANC-inoculated animals presented detectable anti-NS1 IgG. Protection against DENV-2 was verified in pcTPANS1- and pcENS1-immunized mice, although the plasmid pcTPANS1 induced slight higher protective immunity. These plasmids seem to activate distinct patterns of the immune system. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/17020777/DNA_vaccines_against_dengue_virus_based_on_the_ns1_gene:_the_influence_of_different_signal_sequences_on_the_protein_expression_and_its_correlation_to_the_immune_response_elicited_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(06)00624-6 DB - PRIME DP - Unbound Medicine ER -