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Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis.
J Pharm Biomed Anal. 2007 Feb 19; 43(3):873-8.JP

Abstract

A simple, selective, and cost effective capillary zone electrophoresis (CZE) method has been developed for the simultaneous separation and determination of cetirizine dihydrochloride (CTZ), paracetamol (PARA), and phenylpropanolamine hydrochloride (PPA) in tablets. A 10 mM sodium tetraborate background electrolyte (BGE) solution (pH 9.0) was found to be suitable for separation of all the analytes. An uncoated fused-silica capillary of a total length of 76 cm (effective length 64.5 cm) was used for separation. All the analytes were completely separated within 10 min at the applied voltage of 20 kV (current produced approximately 21 microA), and detection was performed at 195 nm with an UV detector. Ibuprofen was used as internal standard (I.S.) for the quantification of the drugs. Validation of the method was performed in terms of linearity, accuracy, precision, limit of detection (LOD), and quantification (LOQ). The linearity of the calibration curves for CTZ, PARA, and PPA (tested range) were 2-50 microg ml(-1) (r(2)=0.9982), 10-1000 microg ml(-1) (r(2)=0.9978), and 10-100 microg ml(-1) (r(2)=0.9986), respectively. The proposed method has been applied for the determination of active ingredients in tablets, and the recovery was found to be > or =98.60% with the relative standard deviation (R.S.D.) < or =1.56%. The LOQ of the CTZ, PARA, and PPA was found to be 2.0, 2.0, and 4.0 microg ml(-1), respectively. There were no interfering peaks due to the excipients present in the pharmaceutical tablets. Thus, the proposed method is simple and suitable for the simultaneous analysis of active ingredients in tablet dosage forms.

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Authors+Show Affiliations

Azhagvuel S
Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad 500007, India.
Sekar R
No affiliation info available

MeSH

AcetaminophenAdrenergic alpha-AgonistsAnalgesics, Non-NarcoticBoratesBuffersCapillary ElectrochromatographyCetirizineChromatography, High Pressure LiquidDrug CombinationsDrug StabilityElectrochemistryHistamine H1 AntagonistsHydrogen-Ion ConcentrationIndicators and ReagentsPhenylpropanolamineReference StandardsReproducibility of ResultsSpectrophotometry, UltravioletTablets

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17023136

Citation

Azhagvuel, S, and R Sekar. "Method Development and Validation for the Simultaneous Determination of Cetirizine Dihydrochloride, Paracetamol, and Phenylpropanolamine Hydrochloride in Tablets By Capillary Zone Electrophoresis." Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 3, 2007, pp. 873-8.
Azhagvuel S, Sekar R. Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis. J Pharm Biomed Anal. 2007;43(3):873-8.
Azhagvuel, S., & Sekar, R. (2007). Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 43(3), 873-8.
Azhagvuel S, Sekar R. Method Development and Validation for the Simultaneous Determination of Cetirizine Dihydrochloride, Paracetamol, and Phenylpropanolamine Hydrochloride in Tablets By Capillary Zone Electrophoresis. J Pharm Biomed Anal. 2007 Feb 19;43(3):873-8. PubMed PMID: 17023136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis. AU - Azhagvuel,S, AU - Sekar,R, Y1 - 2006/10/04/ PY - 2006/05/22/received PY - 2006/08/10/revised PY - 2006/08/31/accepted PY - 2006/10/7/pubmed PY - 2007/4/17/medline PY - 2006/10/7/entrez SP - 873 EP - 8 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 43 IS - 3 N2 - A simple, selective, and cost effective capillary zone electrophoresis (CZE) method has been developed for the simultaneous separation and determination of cetirizine dihydrochloride (CTZ), paracetamol (PARA), and phenylpropanolamine hydrochloride (PPA) in tablets. A 10 mM sodium tetraborate background electrolyte (BGE) solution (pH 9.0) was found to be suitable for separation of all the analytes. An uncoated fused-silica capillary of a total length of 76 cm (effective length 64.5 cm) was used for separation. All the analytes were completely separated within 10 min at the applied voltage of 20 kV (current produced approximately 21 microA), and detection was performed at 195 nm with an UV detector. Ibuprofen was used as internal standard (I.S.) for the quantification of the drugs. Validation of the method was performed in terms of linearity, accuracy, precision, limit of detection (LOD), and quantification (LOQ). The linearity of the calibration curves for CTZ, PARA, and PPA (tested range) were 2-50 microg ml(-1) (r(2)=0.9982), 10-1000 microg ml(-1) (r(2)=0.9978), and 10-100 microg ml(-1) (r(2)=0.9986), respectively. The proposed method has been applied for the determination of active ingredients in tablets, and the recovery was found to be > or =98.60% with the relative standard deviation (R.S.D.) < or =1.56%. The LOQ of the CTZ, PARA, and PPA was found to be 2.0, 2.0, and 4.0 microg ml(-1), respectively. There were no interfering peaks due to the excipients present in the pharmaceutical tablets. Thus, the proposed method is simple and suitable for the simultaneous analysis of active ingredients in tablet dosage forms. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17023136/Method_development_and_validation_for_the_simultaneous_determination_of_cetirizine_dihydrochloride_paracetamol_and_phenylpropanolamine_hydrochloride_in_tablets_by_capillary_zone_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(06)00610-8 DB - PRIME DP - Unbound Medicine ER -