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Estrogen and isoflavone attenuate stress-induced gastric mucosal injury by inhibiting decreases in gastric tissue levels of CGRP in ovariectomized rats.
Am J Physiol Gastrointest Liver Physiol. 2007 Feb; 292(2):G615-9.AJ

Abstract

We have previously reported that CGRP plays a critical role in the reduction of stress-induced gastric mucosal injury by increasing gastric prostacyclin (PGI(2)) levels in rats. Estrogen has been shown to increase the production of CGRP in sensory neurons. Isoflavone has estrogen-like effects and is referred to as a phytoestrogen. Thus, we hypothesized that estrogen and isoflavone might inhibit ovariectomy (OVX)-induced decreases in gastric tissue levels of CGRP, thereby attenuating gastric mucosal injury. We examined these possibilities in the present study. The administration of estradiol and isoflavone for 4 wk completely reversed OVX-induced decreases in CGRP mRNA levels of dorsal root ganglion neurons (DRGs) in rats. OVX-induced decreases in gastric tissue levels of CGRP and 6-keto-PGF(1alpha), a stable metabolite of PGI(2), in rats were reversed by estradiol and isoflavone. Water-immersion restraint stress (WIR)-induced increases in gastric tissue levels of CGRP and 6-keto-PGF(1alpha) were inhibited in ovariectomized rats. This inhibition was completely reversed by estradiol and was partially, but significantly, reversed by isoflavone. WIR-induced gastric mucosal injury was exacerbated by OVX, which was reversed by estradiol and isofolavone. In vitro experiments using DRGs isolated from rats demonstrated that neither estradiol nor isoflavone enhanced CGRP release from DRGs, but the former enhanced it in the presence of anandamide, an endogenous agonist for vanilloid receptor-1. These observations suggest that estrogen and isoflavone might inhibit OVX-induced decreases in CGRP levels in DRGs by promoting transcription, thereby contributing to the attenuation of stress-induced gastric mucosal injury in OVX rats.

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Authors+Show Affiliations

Shimozawa N
Dept of Biodefense Medicine, Nagoya City Univ Graduate School of Medical Sciences, Nagoya, Japan.
Okajima K
No affiliation info available
Harada N
No affiliation info available

MeSH

6-Ketoprostaglandin F1 alphaAnimalsArachidonic AcidsCalcitonin Gene-Related PeptideCannabinoid Receptor ModulatorsCells, CulturedEndocannabinoidsEnzyme-Linked Immunosorbent AssayEstradiolEstrogensFemaleGanglia, SpinalGastric MucosaGene ExpressionIsoflavonesMenstrual CycleNeurons, AfferentOvariectomyPolyunsaturated AlkamidesRNA, MessengerRatsRats, WistarReverse Transcriptase Polymerase Chain ReactionStomachStomach UlcerStress, Physiological

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17023553

Citation

Shimozawa, Nobuhiko, et al. "Estrogen and Isoflavone Attenuate Stress-induced Gastric Mucosal Injury By Inhibiting Decreases in Gastric Tissue Levels of CGRP in Ovariectomized Rats." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 292, no. 2, 2007, pp. G615-9.
Shimozawa N, Okajima K, Harada N. Estrogen and isoflavone attenuate stress-induced gastric mucosal injury by inhibiting decreases in gastric tissue levels of CGRP in ovariectomized rats. Am J Physiol Gastrointest Liver Physiol. 2007;292(2):G615-9.
Shimozawa, N., Okajima, K., & Harada, N. (2007). Estrogen and isoflavone attenuate stress-induced gastric mucosal injury by inhibiting decreases in gastric tissue levels of CGRP in ovariectomized rats. American Journal of Physiology. Gastrointestinal and Liver Physiology, 292(2), G615-9.
Shimozawa N, Okajima K, Harada N. Estrogen and Isoflavone Attenuate Stress-induced Gastric Mucosal Injury By Inhibiting Decreases in Gastric Tissue Levels of CGRP in Ovariectomized Rats. Am J Physiol Gastrointest Liver Physiol. 2007;292(2):G615-9. PubMed PMID: 17023553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen and isoflavone attenuate stress-induced gastric mucosal injury by inhibiting decreases in gastric tissue levels of CGRP in ovariectomized rats. AU - Shimozawa,Nobuhiko, AU - Okajima,Kenji, AU - Harada,Naoaki, Y1 - 2006/10/05/ PY - 2006/10/7/pubmed PY - 2007/4/17/medline PY - 2006/10/7/entrez SP - G615 EP - 9 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 292 IS - 2 N2 - We have previously reported that CGRP plays a critical role in the reduction of stress-induced gastric mucosal injury by increasing gastric prostacyclin (PGI(2)) levels in rats. Estrogen has been shown to increase the production of CGRP in sensory neurons. Isoflavone has estrogen-like effects and is referred to as a phytoestrogen. Thus, we hypothesized that estrogen and isoflavone might inhibit ovariectomy (OVX)-induced decreases in gastric tissue levels of CGRP, thereby attenuating gastric mucosal injury. We examined these possibilities in the present study. The administration of estradiol and isoflavone for 4 wk completely reversed OVX-induced decreases in CGRP mRNA levels of dorsal root ganglion neurons (DRGs) in rats. OVX-induced decreases in gastric tissue levels of CGRP and 6-keto-PGF(1alpha), a stable metabolite of PGI(2), in rats were reversed by estradiol and isoflavone. Water-immersion restraint stress (WIR)-induced increases in gastric tissue levels of CGRP and 6-keto-PGF(1alpha) were inhibited in ovariectomized rats. This inhibition was completely reversed by estradiol and was partially, but significantly, reversed by isoflavone. WIR-induced gastric mucosal injury was exacerbated by OVX, which was reversed by estradiol and isofolavone. In vitro experiments using DRGs isolated from rats demonstrated that neither estradiol nor isoflavone enhanced CGRP release from DRGs, but the former enhanced it in the presence of anandamide, an endogenous agonist for vanilloid receptor-1. These observations suggest that estrogen and isoflavone might inhibit OVX-induced decreases in CGRP levels in DRGs by promoting transcription, thereby contributing to the attenuation of stress-induced gastric mucosal injury in OVX rats. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/17023553/Estrogen_and_isoflavone_attenuate_stress_induced_gastric_mucosal_injury_by_inhibiting_decreases_in_gastric_tissue_levels_of_CGRP_in_ovariectomized_rats_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00117.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -