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Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure.
Parasitol Res. 2007 Mar; 100(4):783-90.PR

Abstract

In the present work, we have investigated the effect of essential oils obtained from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) on growth and ultrastructure of diverse evolutive forms of Trypanosoma cruzi. Culture epimastigotes and bloodstream trypomastigotes were incubated for 24 h with different concentrations of oregano or thyme essential oils and with thymol (the main constituent of thyme), and the inhibitory concentration (IC)(50) was determined by cell counting. Crude extract of oregano essential oil inhibited epimastigote growth (IC(50)/24 h = 175 microg/ml) and also induced trypomastigote lysis (IC(50)/24 h = 115 microg/ml). Thyme essential oil presented IC(50)/24 h values of 77 microg/ml for epimastigotes and 38 mug/ml for trypomastigotes, while treatment with thymol resulted in an IC(50)/24 h of 62 microg/ml for epimastigotes and 53 microg/ml for trypomastigotes. Scanning electron microscopy of treated cells showed few morphological alterations at the plasma membrane. Observation by transmission electron microscopy showed cytoplasmic swelling with occasional morphological alterations in plasma and flagellar membrane. Our data indicate that oregano and thyme essential oils are effective against T. cruzi, with higher activity of thyme, and that thymol may be the main component responsible for the trypanocidal activity.

Authors+Show Affiliations

Laboratório de Biologia Celular de Microrganismos, Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365 Manguinhos, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17024354

Citation

Santoro, Giani F., et al. "Effect of Oregano (Origanum Vulgare L.) and Thyme (Thymus Vulgaris L.) Essential Oils On Trypanosoma Cruzi (Protozoa: Kinetoplastida) Growth and Ultrastructure." Parasitology Research, vol. 100, no. 4, 2007, pp. 783-90.
Santoro GF, das Graças Cardoso M, Guimarães LG, et al. Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure. Parasitol Res. 2007;100(4):783-90.
Santoro, G. F., das Graças Cardoso, M., Guimarães, L. G., Salgado, A. P., Menna-Barreto, R. F., & Soares, M. J. (2007). Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure. Parasitology Research, 100(4), 783-90.
Santoro GF, et al. Effect of Oregano (Origanum Vulgare L.) and Thyme (Thymus Vulgaris L.) Essential Oils On Trypanosoma Cruzi (Protozoa: Kinetoplastida) Growth and Ultrastructure. Parasitol Res. 2007;100(4):783-90. PubMed PMID: 17024354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure. AU - Santoro,Giani F, AU - das Graças Cardoso,Maria, AU - Guimarães,Luiz Gustavo L, AU - Salgado,Ana Paula S P, AU - Menna-Barreto,Rubem F S, AU - Soares,Maurilio J, Y1 - 2006/10/06/ PY - 2006/07/31/received PY - 2006/08/28/accepted PY - 2006/10/7/pubmed PY - 2007/9/29/medline PY - 2006/10/7/entrez SP - 783 EP - 90 JF - Parasitology research JO - Parasitol Res VL - 100 IS - 4 N2 - In the present work, we have investigated the effect of essential oils obtained from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) on growth and ultrastructure of diverse evolutive forms of Trypanosoma cruzi. Culture epimastigotes and bloodstream trypomastigotes were incubated for 24 h with different concentrations of oregano or thyme essential oils and with thymol (the main constituent of thyme), and the inhibitory concentration (IC)(50) was determined by cell counting. Crude extract of oregano essential oil inhibited epimastigote growth (IC(50)/24 h = 175 microg/ml) and also induced trypomastigote lysis (IC(50)/24 h = 115 microg/ml). Thyme essential oil presented IC(50)/24 h values of 77 microg/ml for epimastigotes and 38 mug/ml for trypomastigotes, while treatment with thymol resulted in an IC(50)/24 h of 62 microg/ml for epimastigotes and 53 microg/ml for trypomastigotes. Scanning electron microscopy of treated cells showed few morphological alterations at the plasma membrane. Observation by transmission electron microscopy showed cytoplasmic swelling with occasional morphological alterations in plasma and flagellar membrane. Our data indicate that oregano and thyme essential oils are effective against T. cruzi, with higher activity of thyme, and that thymol may be the main component responsible for the trypanocidal activity. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/17024354/Effect_of_oregano__Origanum_vulgare_L___and_thyme__Thymus_vulgaris_L___essential_oils_on_Trypanosoma_cruzi__Protozoa:_Kinetoplastida__growth_and_ultrastructure_ L2 - https://dx.doi.org/10.1007/s00436-006-0326-5 DB - PRIME DP - Unbound Medicine ER -