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Electrical induction of spikes in the hippocampus impairs recognition capacity and spatial memory in rats.
Epilepsy Behav. 2006 Dec; 9(4):549-56.EB

Abstract

In clinical studies, interictal EEG spikes (IS) have been associated with numerous neuropsychological abnormalities, ranging from transitory cognitive impairment to epileptic encephalopathies. Understanding the pathophysiological mechanisms of IS has been hampered by the lack of validated animal models. To mimic IS, a stimulating microelectrode was implanted in the ventral hippocampal commissure and a recording microelectrode in the CA1 region of the hippocampus of normal male rats. Spike patterns were induced using a series of electrical pulses 10-30 ms in duration with a frequency of 0.5-2Hz and a current of 0.2mA. The commissural stimulation-evoked population discharges in the hippocampus resembled naturally occurring IS in epileptic rats and, in no cases, resulted in behavioral seizures. For behavioral testing, the Morris water maze, radial arm maze, and object recognition tasks were used. Spikes were induced during sleep between the two sets of water maze trials; during the trials in the radial arm maze task; and prior to the sample phase and during the delay periods in the object recognition task. We demonstrated that rats that received spikes took longer to reach the escape platform in the second set of water maze trials; had significantly more reference errors and required more trials to complete the radial arm maze task; and had lower investigation ratios in the object recognition task. The results indicate that induction of spikes in the hippocampus results in impairment of spatial reference and nonspatial object recognition memory.

Authors+Show Affiliations

Neuroscience Center at Dartmouth, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756-001, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17027341

Citation

Shatskikh, Tatiana N., et al. "Electrical Induction of Spikes in the Hippocampus Impairs Recognition Capacity and Spatial Memory in Rats." Epilepsy & Behavior : E&B, vol. 9, no. 4, 2006, pp. 549-56.
Shatskikh TN, Raghavendra M, Zhao Q, et al. Electrical induction of spikes in the hippocampus impairs recognition capacity and spatial memory in rats. Epilepsy Behav. 2006;9(4):549-56.
Shatskikh, T. N., Raghavendra, M., Zhao, Q., Cui, Z., & Holmes, G. L. (2006). Electrical induction of spikes in the hippocampus impairs recognition capacity and spatial memory in rats. Epilepsy & Behavior : E&B, 9(4), 549-56.
Shatskikh TN, et al. Electrical Induction of Spikes in the Hippocampus Impairs Recognition Capacity and Spatial Memory in Rats. Epilepsy Behav. 2006;9(4):549-56. PubMed PMID: 17027341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrical induction of spikes in the hippocampus impairs recognition capacity and spatial memory in rats. AU - Shatskikh,Tatiana N, AU - Raghavendra,Meghana, AU - Zhao,Qian, AU - Cui,Zhiyong, AU - Holmes,Gregory L, Y1 - 2006/10/05/ PY - 2006/07/20/received PY - 2006/08/22/revised PY - 2006/08/25/accepted PY - 2006/10/10/pubmed PY - 2007/1/19/medline PY - 2006/10/10/entrez SP - 549 EP - 56 JF - Epilepsy & behavior : E&B JO - Epilepsy Behav VL - 9 IS - 4 N2 - In clinical studies, interictal EEG spikes (IS) have been associated with numerous neuropsychological abnormalities, ranging from transitory cognitive impairment to epileptic encephalopathies. Understanding the pathophysiological mechanisms of IS has been hampered by the lack of validated animal models. To mimic IS, a stimulating microelectrode was implanted in the ventral hippocampal commissure and a recording microelectrode in the CA1 region of the hippocampus of normal male rats. Spike patterns were induced using a series of electrical pulses 10-30 ms in duration with a frequency of 0.5-2Hz and a current of 0.2mA. The commissural stimulation-evoked population discharges in the hippocampus resembled naturally occurring IS in epileptic rats and, in no cases, resulted in behavioral seizures. For behavioral testing, the Morris water maze, radial arm maze, and object recognition tasks were used. Spikes were induced during sleep between the two sets of water maze trials; during the trials in the radial arm maze task; and prior to the sample phase and during the delay periods in the object recognition task. We demonstrated that rats that received spikes took longer to reach the escape platform in the second set of water maze trials; had significantly more reference errors and required more trials to complete the radial arm maze task; and had lower investigation ratios in the object recognition task. The results indicate that induction of spikes in the hippocampus results in impairment of spatial reference and nonspatial object recognition memory. SN - 1525-5050 UR - https://www.unboundmedicine.com/medline/citation/17027341/Electrical_induction_of_spikes_in_the_hippocampus_impairs_recognition_capacity_and_spatial_memory_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1525-5050(06)00340-4 DB - PRIME DP - Unbound Medicine ER -