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T cell lines specific for an immunodominant epitope of human basic protein define an encephalitogenic determinant for experimental autoimmune encephalomyelitis-resistant LOU/M rats.
J Immunol. 1991 Jan 15; 146(2):515-20.JI

Abstract

The LOU/M rat (RT-1w) haplotype, although resistant to an encephalitogenic challenge of guinea pig myelin basic protein (Gp-BP)/CFA and unresponsive to Gp-BP, responded strongly to human (Hu)-BP. Both T cell and antibody responses focused on the 110-129 determinant of Hu-BP, and T cells specific for this epitope transferred clinical and histologic experimental autoimmune encephalomyelitis (EAE) to naive LOU/M rats. Moreover, EAE could be induced actively with Hu-BP and a synthetic Hu-S110-129 peptide in CFA, but only with co-immunomodulation by pertussis toxin or cyclophosphamide. Analysis of TCR V region genes revealed the predominant use of the V beta 8.5-J beta 2.3 gene combination, with extensive N region additions to both D beta 1 and D beta 2. These results define the Hu-BP 110-129 peptide sequence as the major encephalitogenic epitope for the LOU/M strain of rat previously considered resistant to EAE, and support the idea that the encephalitogenic property of BP and other CNS Ag for a given MHC is encompassed within immunodominant T cell epitopes. Furthermore, the TCR sequence data indicate the predominant use of a different V beta 8 subfamily member (V beta 8.5) than the V beta 8.2 gene used preferentially by several other rat strains and the PL/J mouse in the T cell response to BP.

Authors+Show Affiliations

Department of Microbiology and Surgery, St. Luke's-Roosevelt Hospital Center, New York, NY.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1702803

Citation

Hashim, G, et al. "T Cell Lines Specific for an Immunodominant Epitope of Human Basic Protein Define an Encephalitogenic Determinant for Experimental Autoimmune Encephalomyelitis-resistant LOU/M Rats." Journal of Immunology (Baltimore, Md. : 1950), vol. 146, no. 2, 1991, pp. 515-20.
Hashim G, Vandenbark AA, Gold DP, et al. T cell lines specific for an immunodominant epitope of human basic protein define an encephalitogenic determinant for experimental autoimmune encephalomyelitis-resistant LOU/M rats. J Immunol. 1991;146(2):515-20.
Hashim, G., Vandenbark, A. A., Gold, D. P., Diamanduros, T., & Offner, H. (1991). T cell lines specific for an immunodominant epitope of human basic protein define an encephalitogenic determinant for experimental autoimmune encephalomyelitis-resistant LOU/M rats. Journal of Immunology (Baltimore, Md. : 1950), 146(2), 515-20.
Hashim G, et al. T Cell Lines Specific for an Immunodominant Epitope of Human Basic Protein Define an Encephalitogenic Determinant for Experimental Autoimmune Encephalomyelitis-resistant LOU/M Rats. J Immunol. 1991 Jan 15;146(2):515-20. PubMed PMID: 1702803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - T cell lines specific for an immunodominant epitope of human basic protein define an encephalitogenic determinant for experimental autoimmune encephalomyelitis-resistant LOU/M rats. AU - Hashim,G, AU - Vandenbark,A A, AU - Gold,D P, AU - Diamanduros,T, AU - Offner,H, PY - 1991/1/15/pubmed PY - 1991/1/15/medline PY - 1991/1/15/entrez SP - 515 EP - 20 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 146 IS - 2 N2 - The LOU/M rat (RT-1w) haplotype, although resistant to an encephalitogenic challenge of guinea pig myelin basic protein (Gp-BP)/CFA and unresponsive to Gp-BP, responded strongly to human (Hu)-BP. Both T cell and antibody responses focused on the 110-129 determinant of Hu-BP, and T cells specific for this epitope transferred clinical and histologic experimental autoimmune encephalomyelitis (EAE) to naive LOU/M rats. Moreover, EAE could be induced actively with Hu-BP and a synthetic Hu-S110-129 peptide in CFA, but only with co-immunomodulation by pertussis toxin or cyclophosphamide. Analysis of TCR V region genes revealed the predominant use of the V beta 8.5-J beta 2.3 gene combination, with extensive N region additions to both D beta 1 and D beta 2. These results define the Hu-BP 110-129 peptide sequence as the major encephalitogenic epitope for the LOU/M strain of rat previously considered resistant to EAE, and support the idea that the encephalitogenic property of BP and other CNS Ag for a given MHC is encompassed within immunodominant T cell epitopes. Furthermore, the TCR sequence data indicate the predominant use of a different V beta 8 subfamily member (V beta 8.5) than the V beta 8.2 gene used preferentially by several other rat strains and the PL/J mouse in the T cell response to BP. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1702803/T_cell_lines_specific_for_an_immunodominant_epitope_of_human_basic_protein_define_an_encephalitogenic_determinant_for_experimental_autoimmune_encephalomyelitis_resistant_LOU/M_rats_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1702803 DB - PRIME DP - Unbound Medicine ER -