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Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle.
Dev Dyn. 2006 Dec; 235(12):3230-9.DD

Abstract

Satellite cells are the myogenic cells lying between the myofiber sarcolemma and basal lamina. The objective of this study was to determine the expression patterns of MyoD, myogenin, and Pax7 within the satellite cell population in the growing rat soleus and extensor digitorum longus (EDL) muscles. Secondly, the expression of the myogenic markers was also studied within the interstitial cell compartment and myonuclei. It was discovered that the soleus contained a higher number of Pax7, MyoD, or myogenin-positive nuclei compared with the EDL. Similarly, myogenin was expressed at a lower level in the myonuclei of the soleus compared with the EDL, and myogenin was expressed at a higher level in the interstitial compartment of the soleus compared with the EDL. When interstitial nuclei, myonuclei, and double-labeled nuclei were used in the estimate of the satellite cell population, it was discovered that approximately of 13% of the myofibers in a transverse section of the soleus muscle and 4.1% of EDL myofibers exhibit a labeled satellite cell nucleus. Overall, results from this study suggest that expression patterns of these markers vary predictably among muscles with different growth dynamics and phenotypic characteristics.

Authors+Show Affiliations

Department of Anatomy, University of Wisconsin, Madison Wisconsin, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17029285

Citation

Schultz, Edward, et al. "Satellite Cells Express Distinct Patterns of Myogenic Proteins in Immature Skeletal Muscle." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 235, no. 12, 2006, pp. 3230-9.
Schultz E, Chamberlain C, McCormick KM, et al. Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle. Dev Dyn. 2006;235(12):3230-9.
Schultz, E., Chamberlain, C., McCormick, K. M., & Mozdziak, P. E. (2006). Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 235(12), 3230-9.
Schultz E, et al. Satellite Cells Express Distinct Patterns of Myogenic Proteins in Immature Skeletal Muscle. Dev Dyn. 2006;235(12):3230-9. PubMed PMID: 17029285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle. AU - Schultz,Edward, AU - Chamberlain,Connie, AU - McCormick,Kathleen M, AU - Mozdziak,Paul E, PY - 2006/10/10/pubmed PY - 2007/3/7/medline PY - 2006/10/10/entrez SP - 3230 EP - 9 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 235 IS - 12 N2 - Satellite cells are the myogenic cells lying between the myofiber sarcolemma and basal lamina. The objective of this study was to determine the expression patterns of MyoD, myogenin, and Pax7 within the satellite cell population in the growing rat soleus and extensor digitorum longus (EDL) muscles. Secondly, the expression of the myogenic markers was also studied within the interstitial cell compartment and myonuclei. It was discovered that the soleus contained a higher number of Pax7, MyoD, or myogenin-positive nuclei compared with the EDL. Similarly, myogenin was expressed at a lower level in the myonuclei of the soleus compared with the EDL, and myogenin was expressed at a higher level in the interstitial compartment of the soleus compared with the EDL. When interstitial nuclei, myonuclei, and double-labeled nuclei were used in the estimate of the satellite cell population, it was discovered that approximately of 13% of the myofibers in a transverse section of the soleus muscle and 4.1% of EDL myofibers exhibit a labeled satellite cell nucleus. Overall, results from this study suggest that expression patterns of these markers vary predictably among muscles with different growth dynamics and phenotypic characteristics. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/17029285/Satellite_cells_express_distinct_patterns_of_myogenic_proteins_in_immature_skeletal_muscle_ L2 - https://doi.org/10.1002/dvdy.20976 DB - PRIME DP - Unbound Medicine ER -