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[Performance of different methods of oxacillin resistance detection in atypic strains of Staphylococcus aureus].
Pathol Biol (Paris). 2006 Oct-Nov; 54(8-9):447-52.PB

Abstract

Seventy-three of aminoglycoside-susceptible methicillin-resistant Staphylococcus aureus (AS-MRSA) and 12 kanamycin-tobramycin-resistant methicillin-susceptible S. aureus (KTR-MSSA) isolates were phenotypically and genotypically examined for methicillin susceptibility. The AS-MRSA profile represents 8.3% of MRSA strains and the KTR-MSSA profile represents 1.38% of MSSA strains. The diffusion method using the 5 microg oxacillin and 30 microg cefoxitin discs on Mueller-Hinton Agar (MHA) with and without NaCl, the incubation at 35 degrees C or 30 degrees C for 24 or 48 hours respectively, and the determining oxacillin MICs by E-test (AES, Combourg, France) were performed and used as phenotypic methods. We also used the mecA gene PCR which was considered as the "gold standard" for methicillin resistance detection, and the Slidex MRSA Detection (bioMérieux) that detect the presence of mecA gene product (PBP 2a). To increase the level of PBP 2a expression, the 30 microg cefoxitin disc was used as an inducer. All the AS-MRSA strains (100%) were detected by the cefoxitin disc in all conditions and by the oxacillin disc on MHA with 2% of NaCl at 35 degrees C. Without NaCl, the sensitivity fell to 97,2% by oxacillin disc. The oxacillin MICs for these isolates ranged from 2 to 128 mg/l. The mecA gene determinant and its product PBP 2a were detected in all AS-MRSA strains. All KTR-MSSA strains were phenotypically methicillin-susceptible and oxacillin MICs were below or borderline of breakpoint (< or =2 mg/l). The mecA gene determinant and its product were detected in one strain which was considered to be the most heterogeneous of those tested.

Authors+Show Affiliations

Service de bactériologie-hygiène, CHU d'Amiens, France. Hamdad-Daoudi.Farida@chu-amiens.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

fre

PubMed ID

17030459

Citation

Hamdad, F, et al. "[Performance of Different Methods of Oxacillin Resistance Detection in Atypic Strains of Staphylococcus Aureus]." Pathologie-biologie, vol. 54, no. 8-9, 2006, pp. 447-52.
Hamdad F, Donda F, Laurans G, et al. [Performance of different methods of oxacillin resistance detection in atypic strains of Staphylococcus aureus]. Pathol Biol (Paris). 2006;54(8-9):447-52.
Hamdad, F., Donda, F., Laurans, G., Canarelli, B., Rousseau, F., Biendo, M., Thomas, D., & Eb, F. (2006). [Performance of different methods of oxacillin resistance detection in atypic strains of Staphylococcus aureus]. Pathologie-biologie, 54(8-9), 447-52.
Hamdad F, et al. [Performance of Different Methods of Oxacillin Resistance Detection in Atypic Strains of Staphylococcus Aureus]. Pathol Biol (Paris). 2006 Oct-Nov;54(8-9):447-52. PubMed PMID: 17030459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Performance of different methods of oxacillin resistance detection in atypic strains of Staphylococcus aureus]. AU - Hamdad,F, AU - Donda,F, AU - Laurans,G, AU - Canarelli,B, AU - Rousseau,F, AU - Biendo,M, AU - Thomas,D, AU - Eb,F, Y1 - 2006/10/06/ PY - 2006/05/31/received PY - 2006/07/18/accepted PY - 2006/10/13/pubmed PY - 2007/1/31/medline PY - 2006/10/13/entrez SP - 447 EP - 52 JF - Pathologie-biologie JO - Pathol Biol (Paris) VL - 54 IS - 8-9 N2 - Seventy-three of aminoglycoside-susceptible methicillin-resistant Staphylococcus aureus (AS-MRSA) and 12 kanamycin-tobramycin-resistant methicillin-susceptible S. aureus (KTR-MSSA) isolates were phenotypically and genotypically examined for methicillin susceptibility. The AS-MRSA profile represents 8.3% of MRSA strains and the KTR-MSSA profile represents 1.38% of MSSA strains. The diffusion method using the 5 microg oxacillin and 30 microg cefoxitin discs on Mueller-Hinton Agar (MHA) with and without NaCl, the incubation at 35 degrees C or 30 degrees C for 24 or 48 hours respectively, and the determining oxacillin MICs by E-test (AES, Combourg, France) were performed and used as phenotypic methods. We also used the mecA gene PCR which was considered as the "gold standard" for methicillin resistance detection, and the Slidex MRSA Detection (bioMérieux) that detect the presence of mecA gene product (PBP 2a). To increase the level of PBP 2a expression, the 30 microg cefoxitin disc was used as an inducer. All the AS-MRSA strains (100%) were detected by the cefoxitin disc in all conditions and by the oxacillin disc on MHA with 2% of NaCl at 35 degrees C. Without NaCl, the sensitivity fell to 97,2% by oxacillin disc. The oxacillin MICs for these isolates ranged from 2 to 128 mg/l. The mecA gene determinant and its product PBP 2a were detected in all AS-MRSA strains. All KTR-MSSA strains were phenotypically methicillin-susceptible and oxacillin MICs were below or borderline of breakpoint (< or =2 mg/l). The mecA gene determinant and its product were detected in one strain which was considered to be the most heterogeneous of those tested. SN - 0369-8114 UR - https://www.unboundmedicine.com/medline/citation/17030459/[Performance_of_different_methods_of_oxacillin_resistance_detection_in_atypic_strains_of_Staphylococcus_aureus]_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0369-8114(06)00143-X DB - PRIME DP - Unbound Medicine ER -