Tags

Type your tag names separated by a space and hit enter

Confirmation of peanut protein using peptide markers in dark chocolate using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
J Agric Food Chem. 2006 Oct 18; 54(21):7953-9.JA

Abstract

Detection of peptides from the peanut allergen Ara h 1 by liquid chromatography-mass spectrometry (LC-MS) was used to identify and estimate total peanut protein levels in dark chocolate. A comparison of enzymatic digestion subsequent to and following extraction of Ara h 1 from the food matrix revealed better limits of detection (LOD) for the pre-extraction digestion (20 ppm) than for the postextraction digestion (50 ppm). Evaluation of LC-MS instruments and scan modes showed the LOD could be further reduced to 10 ppm via a triple-quadrupole and multiple-reaction monitoring. Improvements in extraction techniques combined with an increase in the amount of chocolate extracted (1 g) improved the LOD to 2 ppm of peanut protein. This method provides an unambiguous means of confirming the presence of the peanut protein in foods using peptide markers from a major allergen, Ara h 1, and can easily be modified to detect other food allergens.

Authors+Show Affiliations

U.S. Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD 20740, USA. kshefche@cfsan.fda.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17031994

Citation

Shefcheck, Kevin J., et al. "Confirmation of Peanut Protein Using Peptide Markers in Dark Chocolate Using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS)." Journal of Agricultural and Food Chemistry, vol. 54, no. 21, 2006, pp. 7953-9.
Shefcheck KJ, Callahan JH, Musser SM. Confirmation of peanut protein using peptide markers in dark chocolate using liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Agric Food Chem. 2006;54(21):7953-9.
Shefcheck, K. J., Callahan, J. H., & Musser, S. M. (2006). Confirmation of peanut protein using peptide markers in dark chocolate using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Journal of Agricultural and Food Chemistry, 54(21), 7953-9.
Shefcheck KJ, Callahan JH, Musser SM. Confirmation of Peanut Protein Using Peptide Markers in Dark Chocolate Using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). J Agric Food Chem. 2006 Oct 18;54(21):7953-9. PubMed PMID: 17031994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation of peanut protein using peptide markers in dark chocolate using liquid chromatography-tandem mass spectrometry (LC-MS/MS). AU - Shefcheck,Kevin J, AU - Callahan,John H, AU - Musser,Steven M, PY - 2006/10/13/pubmed PY - 2006/12/9/medline PY - 2006/10/13/entrez SP - 7953 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 21 N2 - Detection of peptides from the peanut allergen Ara h 1 by liquid chromatography-mass spectrometry (LC-MS) was used to identify and estimate total peanut protein levels in dark chocolate. A comparison of enzymatic digestion subsequent to and following extraction of Ara h 1 from the food matrix revealed better limits of detection (LOD) for the pre-extraction digestion (20 ppm) than for the postextraction digestion (50 ppm). Evaluation of LC-MS instruments and scan modes showed the LOD could be further reduced to 10 ppm via a triple-quadrupole and multiple-reaction monitoring. Improvements in extraction techniques combined with an increase in the amount of chocolate extracted (1 g) improved the LOD to 2 ppm of peanut protein. This method provides an unambiguous means of confirming the presence of the peanut protein in foods using peptide markers from a major allergen, Ara h 1, and can easily be modified to detect other food allergens. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17031994/Confirmation_of_peanut_protein_using_peptide_markers_in_dark_chocolate_using_liquid_chromatography_tandem_mass_spectrometry__LC_MS/MS__ L2 - https://doi.org/10.1021/jf060714e DB - PRIME DP - Unbound Medicine ER -