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Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties.
J Agric Food Chem. 2006 Oct 18; 54(21):8048-57.JA

Abstract

Response surface methodology (RSM) was used to predict the optimum conditions of extraction of barley samples (organic solvent percent in the extraction medium, temperature, and time). Antioxidant capacity in the barley meals was highest under optimum extraction conditions of 80.2% methanol and 60.5 degrees C for 38.36 min as predicted by RSM. Phenolic antioxidative compounds of six barley cultivars, namely, Falcon, AC Metcalfe, Tercel, Tyto, Phoenix, and Peregrine, were extracted under the conditions obtained by RSM after defatting with hexane, and subsequently the extracts were assessed for their antioxidant and antiradical activities and metal chelation efficacy. The potential of barley extracts in inhibiting peroxyl and hydroxyl radical induced supercoiled DNA double-strand scission was also studied. Total phenolic content as measured according to Folin-Ciocalteu's method ranged from 13.58 to 22.93 mg of ferulic acid equiv/g of defatted material, with the highest content in Peregrine. Total antioxidant activity as measured by Trolox equivalent antioxidant capacity ranged from 3.74 to 6.82 micromol/g of defatted material. Metal chelation capacity of the extracts as measured by 2,2'-bipyridyl competition assay varied from 1.1 to 2.1 micromol of ethylenediaminetetraacetic acid equiv/g of defatted material. IC(50) values for 1,1-diphenyl-2-picrylhydrazyl radical as measured by electron paramagnetic resonance ranged from 1.51 to 3.33 mg/mL, whereas the corresponding values for hydroxyl radical ranged between 2.20 and 9.65 mg/mL. Inhibition of peroxyl radical induced supercoiled DNA scission ranged from 78.2 to 92.1% at the concentration of 4 mg/mL of extracts, whereas the corresponding values for hydroxyl radical induced DNA scission ranged from 53.1 to 65.3%.

Authors+Show Affiliations

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17032008

Citation

Madhujith, Terrence, and Fereidoon Shahidi. "Optimization of the Extraction of Antioxidative Constituents of Six Barley Cultivars and Their Antioxidant Properties." Journal of Agricultural and Food Chemistry, vol. 54, no. 21, 2006, pp. 8048-57.
Madhujith T, Shahidi F. Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties. J Agric Food Chem. 2006;54(21):8048-57.
Madhujith, T., & Shahidi, F. (2006). Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties. Journal of Agricultural and Food Chemistry, 54(21), 8048-57.
Madhujith T, Shahidi F. Optimization of the Extraction of Antioxidative Constituents of Six Barley Cultivars and Their Antioxidant Properties. J Agric Food Chem. 2006 Oct 18;54(21):8048-57. PubMed PMID: 17032008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties. AU - Madhujith,Terrence, AU - Shahidi,Fereidoon, PY - 2006/10/13/pubmed PY - 2006/12/9/medline PY - 2006/10/13/entrez SP - 8048 EP - 57 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 54 IS - 21 N2 - Response surface methodology (RSM) was used to predict the optimum conditions of extraction of barley samples (organic solvent percent in the extraction medium, temperature, and time). Antioxidant capacity in the barley meals was highest under optimum extraction conditions of 80.2% methanol and 60.5 degrees C for 38.36 min as predicted by RSM. Phenolic antioxidative compounds of six barley cultivars, namely, Falcon, AC Metcalfe, Tercel, Tyto, Phoenix, and Peregrine, were extracted under the conditions obtained by RSM after defatting with hexane, and subsequently the extracts were assessed for their antioxidant and antiradical activities and metal chelation efficacy. The potential of barley extracts in inhibiting peroxyl and hydroxyl radical induced supercoiled DNA double-strand scission was also studied. Total phenolic content as measured according to Folin-Ciocalteu's method ranged from 13.58 to 22.93 mg of ferulic acid equiv/g of defatted material, with the highest content in Peregrine. Total antioxidant activity as measured by Trolox equivalent antioxidant capacity ranged from 3.74 to 6.82 micromol/g of defatted material. Metal chelation capacity of the extracts as measured by 2,2'-bipyridyl competition assay varied from 1.1 to 2.1 micromol of ethylenediaminetetraacetic acid equiv/g of defatted material. IC(50) values for 1,1-diphenyl-2-picrylhydrazyl radical as measured by electron paramagnetic resonance ranged from 1.51 to 3.33 mg/mL, whereas the corresponding values for hydroxyl radical ranged between 2.20 and 9.65 mg/mL. Inhibition of peroxyl radical induced supercoiled DNA scission ranged from 78.2 to 92.1% at the concentration of 4 mg/mL of extracts, whereas the corresponding values for hydroxyl radical induced DNA scission ranged from 53.1 to 65.3%. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17032008/Optimization_of_the_extraction_of_antioxidative_constituents_of_six_barley_cultivars_and_their_antioxidant_properties_ L2 - https://dx.doi.org/10.1021/jf061558e DB - PRIME DP - Unbound Medicine ER -