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Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells.
J Agric Food Chem. 2006 Oct 18; 54(21):8058-64.JA

Abstract

In the present study, we successively extracted the Pu-erh tea with acetone, water, chloroform, ethyl acetate, and n-butanol, and the extracts were then isolated by column chromatography. Our study demonstrates that the Pu-erh tea ethyl acetate extract, n-butanol extract, and their fractions had superoxide anion and hydroxyl radical scavenging activity: fractions 2 and 8 from the ethyl acetate extract and fractions 2, 4, and 5 from the n-butanol extract showed protective effects against hydrogen peroxide-induced damage in human fibroblast HPF-1 cells and increased the cells' viability under normal cell culture conditions. In addition, it is found that these fractions, except fraction 5 from the n-butanol extract, decreased the accumulation of intracellular reactive oxygen species in hydrogen peroxide-induced HPF-1 cells. Interestingly, the antioxidant effect of fraction 8 from the ethyl acetate extract on the above four systems was much stronger than that of the typical green tea catechin (-)-epigallocatechin-3-gallate, but there were almost no monomeric polyphenols, theaflavins, and gallic acid in fraction 8.

Authors+Show Affiliations

Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17032009

Citation

Jie, Guoliang, et al. "Free Radical Scavenging Effect of Pu-erh Tea Extracts and Their Protective Effect On Oxidative Damage in Human Fibroblast Cells." Journal of Agricultural and Food Chemistry, vol. 54, no. 21, 2006, pp. 8058-64.
Jie G, Lin Z, Zhang L, et al. Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells. J Agric Food Chem. 2006;54(21):8058-64.
Jie, G., Lin, Z., Zhang, L., Lv, H., He, P., & Zhao, B. (2006). Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells. Journal of Agricultural and Food Chemistry, 54(21), 8058-64.
Jie G, et al. Free Radical Scavenging Effect of Pu-erh Tea Extracts and Their Protective Effect On Oxidative Damage in Human Fibroblast Cells. J Agric Food Chem. 2006 Oct 18;54(21):8058-64. PubMed PMID: 17032009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells. AU - Jie,Guoliang, AU - Lin,Zhi, AU - Zhang,Longze, AU - Lv,Haipeng, AU - He,Puming, AU - Zhao,Baolu, PY - 2006/10/13/pubmed PY - 2006/12/9/medline PY - 2006/10/13/entrez SP - 8058 EP - 64 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 21 N2 - In the present study, we successively extracted the Pu-erh tea with acetone, water, chloroform, ethyl acetate, and n-butanol, and the extracts were then isolated by column chromatography. Our study demonstrates that the Pu-erh tea ethyl acetate extract, n-butanol extract, and their fractions had superoxide anion and hydroxyl radical scavenging activity: fractions 2 and 8 from the ethyl acetate extract and fractions 2, 4, and 5 from the n-butanol extract showed protective effects against hydrogen peroxide-induced damage in human fibroblast HPF-1 cells and increased the cells' viability under normal cell culture conditions. In addition, it is found that these fractions, except fraction 5 from the n-butanol extract, decreased the accumulation of intracellular reactive oxygen species in hydrogen peroxide-induced HPF-1 cells. Interestingly, the antioxidant effect of fraction 8 from the ethyl acetate extract on the above four systems was much stronger than that of the typical green tea catechin (-)-epigallocatechin-3-gallate, but there were almost no monomeric polyphenols, theaflavins, and gallic acid in fraction 8. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17032009/Free_radical_scavenging_effect_of_Pu_erh_tea_extracts_and_their_protective_effect_on_oxidative_damage_in_human_fibroblast_cells_ L2 - https://doi.org/10.1021/jf061663o DB - PRIME DP - Unbound Medicine ER -