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Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart.
Cardiovasc Res. 2006 Dec 01; 72(3):430-7.CR

Abstract

OBJECTIVES

Cardiac fatty acid uptake occurs predominantly via sarcolemmal transporter proteins; fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm) and fatty acid transporter proteins (FATP) 1 and 6. We hypothesised that levels of the fatty acid transporters would be reduced in the chronically infarcted rat heart, in parallel with reduced dependence on fatty acid utilisation.

METHODS AND RESULTS

In vivo left ventricular ejection fractions, measured using echocardiography, were 36% lower in rats six months after coronary artery ligation than in sham-operated control rats. In isolated, perfused, infarcted hearts, 3H-palmitate oxidation was 30% lower, and correlated with in vivo ejection fractions. As myocardial lipid incorporation was also reduced by 25%, total palmitate utilisation was 29% lower in the infarcted rat heart. The protein levels of the cardiac fatty acid transporters were reduced in the infarcted rat heart; FAT/CD36 by 36%, FABPpm by 12%, FATP6 by 21% and FATP1 by 26%, and the cytosolic fatty acid binding protein (cFABP) was 47% lower than in sham-operated rat hearts. Fatty acid transporter levels correlated with both palmitate oxidation rates and cardiac ejection fractions.

CONCLUSIONS

Reductions in fatty acid oxidation and lipid incorporation rates were accompanied by downregulation of the cardiac fatty acid transporters. The metabolic shift away from fatty acid utilisation was proportional to the degree of functional impairment in the chronically infarcted rat heart.

Authors+Show Affiliations

Cardiac Metabolism Research Group, Department of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Parks Road, Oxford, OX1 3PT, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17034771

Citation

Heather, Lisa C., et al. "Fatty Acid Transporter Levels and Palmitate Oxidation Rate Correlate With Ejection Fraction in the Infarcted Rat Heart." Cardiovascular Research, vol. 72, no. 3, 2006, pp. 430-7.
Heather LC, Cole MA, Lygate CA, et al. Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart. Cardiovasc Res. 2006;72(3):430-7.
Heather, L. C., Cole, M. A., Lygate, C. A., Evans, R. D., Stuckey, D. J., Murray, A. J., Neubauer, S., & Clarke, K. (2006). Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart. Cardiovascular Research, 72(3), 430-7.
Heather LC, et al. Fatty Acid Transporter Levels and Palmitate Oxidation Rate Correlate With Ejection Fraction in the Infarcted Rat Heart. Cardiovasc Res. 2006 Dec 1;72(3):430-7. PubMed PMID: 17034771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart. AU - Heather,Lisa C, AU - Cole,Mark A, AU - Lygate,Craig A, AU - Evans,Rhys D, AU - Stuckey,Daniel J, AU - Murray,Andrew J, AU - Neubauer,Stefan, AU - Clarke,Kieran, Y1 - 2006/09/01/ PY - 2006/06/15/received PY - 2006/07/27/revised PY - 2006/08/23/accepted PY - 2006/10/13/pubmed PY - 2007/7/10/medline PY - 2006/10/13/entrez SP - 430 EP - 7 JF - Cardiovascular research JO - Cardiovasc Res VL - 72 IS - 3 N2 - OBJECTIVES: Cardiac fatty acid uptake occurs predominantly via sarcolemmal transporter proteins; fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm) and fatty acid transporter proteins (FATP) 1 and 6. We hypothesised that levels of the fatty acid transporters would be reduced in the chronically infarcted rat heart, in parallel with reduced dependence on fatty acid utilisation. METHODS AND RESULTS: In vivo left ventricular ejection fractions, measured using echocardiography, were 36% lower in rats six months after coronary artery ligation than in sham-operated control rats. In isolated, perfused, infarcted hearts, 3H-palmitate oxidation was 30% lower, and correlated with in vivo ejection fractions. As myocardial lipid incorporation was also reduced by 25%, total palmitate utilisation was 29% lower in the infarcted rat heart. The protein levels of the cardiac fatty acid transporters were reduced in the infarcted rat heart; FAT/CD36 by 36%, FABPpm by 12%, FATP6 by 21% and FATP1 by 26%, and the cytosolic fatty acid binding protein (cFABP) was 47% lower than in sham-operated rat hearts. Fatty acid transporter levels correlated with both palmitate oxidation rates and cardiac ejection fractions. CONCLUSIONS: Reductions in fatty acid oxidation and lipid incorporation rates were accompanied by downregulation of the cardiac fatty acid transporters. The metabolic shift away from fatty acid utilisation was proportional to the degree of functional impairment in the chronically infarcted rat heart. SN - 0008-6363 UR - https://www.unboundmedicine.com/medline/citation/17034771/Fatty_acid_transporter_levels_and_palmitate_oxidation_rate_correlate_with_ejection_fraction_in_the_infarcted_rat_heart_ L2 - https://academic.oup.com/cardiovascres/article-lookup/doi/10.1016/j.cardiores.2006.08.020 DB - PRIME DP - Unbound Medicine ER -