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On-line derivatization gas chromatography with furan chemical ionization tandem mass spectrometry for screening of amphetamines in urine.
J Chromatogr A. 2006 Dec 22; 1137(1):76-83.JC

Abstract

A simple alternative method with minimal sample pretreatment is investigated for screening of amphetamines in small volume (using only 20 microL) of urine sample. The method is sensitive and selective. The method uses gas chromatography (GC) direct sample introduction (DSI) for on-line derivatization (acylation) of amphetamines to improve sensitivity. Furan as chemical ionization (CI) reagent in conjunction with tandem mass spectrometry (MS/MS) is used to improve selectivity. Low background with sharp protonated molecular ion peaks of analytes is the evidence of improvement in sensitivity and selectivity. Blank urine samples spiked with known amounts of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyethylamphetamine is analyzed. Selected ion monitoring of the characteristic product ions (m/z 119+136+150+163) using furan CI-MS/MS in positive ion mode is used for quantification. Limits of detection (LOD) between 0.4 and 1.0 ng mL(-1) and limits of quantitation (LOQ) between 1.0 and 2.0 ng mL(-1) are established. Linear response over the range of 1-1000 ng mL(-1) (r(2)>0.997) is observed for all analytes, except for methamphetamine (2.0-1000 ng mL(-1)). Good accuracy between 86 and 113% and precision ranging from 4 to 18% is obtained. The method is also tested on real samples of urine from suspected drug abusers. This method could be used for screening and determination of amphetamines in urine samples, however needs additional work for full validation.

Authors+Show Affiliations

Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17034801

Citation

Tzing, Shin-Hwa, et al. "On-line Derivatization Gas Chromatography With Furan Chemical Ionization Tandem Mass Spectrometry for Screening of Amphetamines in Urine." Journal of Chromatography. A, vol. 1137, no. 1, 2006, pp. 76-83.
Tzing SH, Ghule A, Liu JY, et al. On-line derivatization gas chromatography with furan chemical ionization tandem mass spectrometry for screening of amphetamines in urine. J Chromatogr A. 2006;1137(1):76-83.
Tzing, S. H., Ghule, A., Liu, J. Y., & Ling, Y. C. (2006). On-line derivatization gas chromatography with furan chemical ionization tandem mass spectrometry for screening of amphetamines in urine. Journal of Chromatography. A, 1137(1), 76-83.
Tzing SH, et al. On-line Derivatization Gas Chromatography With Furan Chemical Ionization Tandem Mass Spectrometry for Screening of Amphetamines in Urine. J Chromatogr A. 2006 Dec 22;1137(1):76-83. PubMed PMID: 17034801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line derivatization gas chromatography with furan chemical ionization tandem mass spectrometry for screening of amphetamines in urine. AU - Tzing,Shin-Hwa, AU - Ghule,Anil, AU - Liu,Jen-Yu, AU - Ling,Yong-Chien, Y1 - 2006/10/10/ PY - 2006/05/18/received PY - 2006/09/22/revised PY - 2006/09/27/accepted PY - 2006/10/13/pubmed PY - 2007/1/16/medline PY - 2006/10/13/entrez SP - 76 EP - 83 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1137 IS - 1 N2 - A simple alternative method with minimal sample pretreatment is investigated for screening of amphetamines in small volume (using only 20 microL) of urine sample. The method is sensitive and selective. The method uses gas chromatography (GC) direct sample introduction (DSI) for on-line derivatization (acylation) of amphetamines to improve sensitivity. Furan as chemical ionization (CI) reagent in conjunction with tandem mass spectrometry (MS/MS) is used to improve selectivity. Low background with sharp protonated molecular ion peaks of analytes is the evidence of improvement in sensitivity and selectivity. Blank urine samples spiked with known amounts of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyethylamphetamine is analyzed. Selected ion monitoring of the characteristic product ions (m/z 119+136+150+163) using furan CI-MS/MS in positive ion mode is used for quantification. Limits of detection (LOD) between 0.4 and 1.0 ng mL(-1) and limits of quantitation (LOQ) between 1.0 and 2.0 ng mL(-1) are established. Linear response over the range of 1-1000 ng mL(-1) (r(2)>0.997) is observed for all analytes, except for methamphetamine (2.0-1000 ng mL(-1)). Good accuracy between 86 and 113% and precision ranging from 4 to 18% is obtained. The method is also tested on real samples of urine from suspected drug abusers. This method could be used for screening and determination of amphetamines in urine samples, however needs additional work for full validation. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17034801/On_line_derivatization_gas_chromatography_with_furan_chemical_ionization_tandem_mass_spectrometry_for_screening_of_amphetamines_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)01875-9 DB - PRIME DP - Unbound Medicine ER -