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Differential proinflammatory and angiogenesis-specific cytokine production in human pulmonary endothelial cells, HPMEC-ST1.6R infected with dengue-2 and dengue-3 virus.
J Virol Methods. 2006 Dec; 138(1-2):211-7.JV

Abstract

In this study, the ability of dengue virus serotypes 2 (DENV-2) and 3 (DENV-3) to infect and induce increased production of proinflammatory cytokines in a pulmonary endothelial cell line (HPMEC-ST1.6R) was investigated. This cell line exhibits the major constitutive and inducible endothelial cell characteristics, as well as angiogenic response. DENV-2 and DENV-3 infection was confirmed by an observed cytopathic effect (CPE), as well as RT-PCR and immunofluorescence assays. Increases in Th-1 and Th-2 cytokines IL-4, IL-8, IL-6, IL-10, GM-CSF, INF-gamma, and tumor necrosis factor (TNF-alpha) within DENV-2- and DENV-3-infected cells were demonstrated using a microbead-based Bio-plex assay. Proinflammatory cytokine increases and the expression of a potent angiogenic inducer protein, VEGF were confirmed by dot-blot analysis using the TranSignal Human Angiogenesis Antibody Array. Dengue virus-infected HPMEC-ST1.6R cells exhibited an elongated cytoplasmic morphology, possibly representing a response to VEGF and activation of angiogenesis. The increased levels of Th-1 cytokines and VEGF in DENV-2 virus infected-HPMEC-ST1.6R could be distinguished from those infected by DENV-3. This suggests that cytokine patterns associated with DENV infections may be serotype and strain-specific. The experimental approaches described here could be developed further into a useful diagnostic tool for the characterization of dengue hemorrhagic fever cases, leading to enhancement of treatment therapy.

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Authors+Show Affiliations

Azizan A
Global Health Department, College of Public Health, 13201 Bruce B Downs Bvld., Tampa, FL 33612, USA. aazizan@hsc.usf.edu
Sweat J
No affiliation info available
Espino C
No affiliation info available
Gemmer J
No affiliation info available
Stark L
No affiliation info available
Kazanis D
No affiliation info available

MeSH

Cell LineCytokinesCytopathogenic Effect, ViralDengue VirusEndothelial CellsFluorescent Antibody TechniqueHumansImmunoblottingLungMicrospheresRNA, ViralReverse Transcriptase Polymerase Chain ReactionVascular Endothelial Growth Factor AViral Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17034872

Citation

Azizan, Azliyati, et al. "Differential Proinflammatory and Angiogenesis-specific Cytokine Production in Human Pulmonary Endothelial Cells, HPMEC-ST1.6R Infected With Dengue-2 and Dengue-3 Virus." Journal of Virological Methods, vol. 138, no. 1-2, 2006, pp. 211-7.
Azizan A, Sweat J, Espino C, et al. Differential proinflammatory and angiogenesis-specific cytokine production in human pulmonary endothelial cells, HPMEC-ST1.6R infected with dengue-2 and dengue-3 virus. J Virol Methods. 2006;138(1-2):211-7.
Azizan, A., Sweat, J., Espino, C., Gemmer, J., Stark, L., & Kazanis, D. (2006). Differential proinflammatory and angiogenesis-specific cytokine production in human pulmonary endothelial cells, HPMEC-ST1.6R infected with dengue-2 and dengue-3 virus. Journal of Virological Methods, 138(1-2), 211-7.
Azizan A, et al. Differential Proinflammatory and Angiogenesis-specific Cytokine Production in Human Pulmonary Endothelial Cells, HPMEC-ST1.6R Infected With Dengue-2 and Dengue-3 Virus. J Virol Methods. 2006;138(1-2):211-7. PubMed PMID: 17034872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential proinflammatory and angiogenesis-specific cytokine production in human pulmonary endothelial cells, HPMEC-ST1.6R infected with dengue-2 and dengue-3 virus. AU - Azizan,Azliyati, AU - Sweat,James, AU - Espino,Carlos, AU - Gemmer,Jennifer, AU - Stark,Lillian, AU - Kazanis,Deno, Y1 - 2006/10/10/ PY - 2006/04/22/received PY - 2006/08/08/revised PY - 2006/08/29/accepted PY - 2006/10/13/pubmed PY - 2007/2/6/medline PY - 2006/10/13/entrez SP - 211 EP - 7 JF - Journal of virological methods JO - J Virol Methods VL - 138 IS - 1-2 N2 - In this study, the ability of dengue virus serotypes 2 (DENV-2) and 3 (DENV-3) to infect and induce increased production of proinflammatory cytokines in a pulmonary endothelial cell line (HPMEC-ST1.6R) was investigated. This cell line exhibits the major constitutive and inducible endothelial cell characteristics, as well as angiogenic response. DENV-2 and DENV-3 infection was confirmed by an observed cytopathic effect (CPE), as well as RT-PCR and immunofluorescence assays. Increases in Th-1 and Th-2 cytokines IL-4, IL-8, IL-6, IL-10, GM-CSF, INF-gamma, and tumor necrosis factor (TNF-alpha) within DENV-2- and DENV-3-infected cells were demonstrated using a microbead-based Bio-plex assay. Proinflammatory cytokine increases and the expression of a potent angiogenic inducer protein, VEGF were confirmed by dot-blot analysis using the TranSignal Human Angiogenesis Antibody Array. Dengue virus-infected HPMEC-ST1.6R cells exhibited an elongated cytoplasmic morphology, possibly representing a response to VEGF and activation of angiogenesis. The increased levels of Th-1 cytokines and VEGF in DENV-2 virus infected-HPMEC-ST1.6R could be distinguished from those infected by DENV-3. This suggests that cytokine patterns associated with DENV infections may be serotype and strain-specific. The experimental approaches described here could be developed further into a useful diagnostic tool for the characterization of dengue hemorrhagic fever cases, leading to enhancement of treatment therapy. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/17034872/Differential_proinflammatory_and_angiogenesis_specific_cytokine_production_in_human_pulmonary_endothelial_cells_HPMEC_ST1_6R_infected_with_dengue_2_and_dengue_3_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(06)00301-6 DB - PRIME DP - Unbound Medicine ER -