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Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms.
Food Chem Toxicol. 2007 Feb; 45(2):210-5.FC

Abstract

Determination of genotoxic effect in fish, micronucleus test as well as the study of the abnormal shape of nuclei is a suitable measure, in which the presence or absence of genotoxins can be detected in water. In the present study, micronuclei and abnormal nuclei frequencies were scored in the gill and kidney erythrocytes of the fish Labeo bata grown in the sewage-fed fish farms of East Calcutta wetlands. Three experimental sites were chosen, namely, Bantala, Chowbaga and Chingrihata (basically these sites have sewage-fed fishponds), which were compared with fishponds of no sewage influence as the control area. Highly significant differences (P<0.001) were noticed for micronucleus frequencies in the gill and kidney erythrocytes of experimental fishes, where kidney erythrocytes showed an increased value than gill erythrocytes without any statistical differences. The frequencies of nuclear abnormalities such as necrotic cells, apoptotic cells, notch nucleated cells and binucleated cells were also counted separately for gill and kidney erythrocytes, in which significantly (P<0.001, P<0.01, P<0.05) increased values were obtained in comparison to control populations. These genotoxicity results confirmed that the sewage-fed ponds contain genotoxic metals such as Cr, Zn, Cu, Pb, Mn, Fe through wastewater and sludge because of the direct use of sewage water without pretreatment which may lead to health risks among humans through chronic consumption of fish from these experimental fish ponds. Other vertebrates grown in sewage-fed ponds may also suffer a certain amount of genotoxic substances.

Authors+Show Affiliations

Department of Environmental Science, Calcutta University College of Science, 35 Ballygunge Circular Road, Kolkata 700 019, India.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17034922

Citation

Talapatra, S N., and S K. Banerjee. "Detection of Micronucleus and Abnormal Nucleus in Erythrocytes From the Gill and Kidney of Labeo Bata Cultivated in Sewage-fed Fish Farms." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 45, no. 2, 2007, pp. 210-5.
Talapatra SN, Banerjee SK. Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms. Food Chem Toxicol. 2007;45(2):210-5.
Talapatra, S. N., & Banerjee, S. K. (2007). Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 45(2), 210-5.
Talapatra SN, Banerjee SK. Detection of Micronucleus and Abnormal Nucleus in Erythrocytes From the Gill and Kidney of Labeo Bata Cultivated in Sewage-fed Fish Farms. Food Chem Toxicol. 2007;45(2):210-5. PubMed PMID: 17034922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms. AU - Talapatra,S N, AU - Banerjee,S K, Y1 - 2006/08/30/ PY - 2005/07/20/received PY - 2006/07/12/revised PY - 2006/07/20/accepted PY - 2006/10/13/pubmed PY - 2007/3/6/medline PY - 2006/10/13/entrez SP - 210 EP - 5 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 45 IS - 2 N2 - Determination of genotoxic effect in fish, micronucleus test as well as the study of the abnormal shape of nuclei is a suitable measure, in which the presence or absence of genotoxins can be detected in water. In the present study, micronuclei and abnormal nuclei frequencies were scored in the gill and kidney erythrocytes of the fish Labeo bata grown in the sewage-fed fish farms of East Calcutta wetlands. Three experimental sites were chosen, namely, Bantala, Chowbaga and Chingrihata (basically these sites have sewage-fed fishponds), which were compared with fishponds of no sewage influence as the control area. Highly significant differences (P<0.001) were noticed for micronucleus frequencies in the gill and kidney erythrocytes of experimental fishes, where kidney erythrocytes showed an increased value than gill erythrocytes without any statistical differences. The frequencies of nuclear abnormalities such as necrotic cells, apoptotic cells, notch nucleated cells and binucleated cells were also counted separately for gill and kidney erythrocytes, in which significantly (P<0.001, P<0.01, P<0.05) increased values were obtained in comparison to control populations. These genotoxicity results confirmed that the sewage-fed ponds contain genotoxic metals such as Cr, Zn, Cu, Pb, Mn, Fe through wastewater and sludge because of the direct use of sewage water without pretreatment which may lead to health risks among humans through chronic consumption of fish from these experimental fish ponds. Other vertebrates grown in sewage-fed ponds may also suffer a certain amount of genotoxic substances. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17034922/Detection_of_micronucleus_and_abnormal_nucleus_in_erythrocytes_from_the_gill_and_kidney_of_Labeo_bata_cultivated_in_sewage_fed_fish_farms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(06)00229-8 DB - PRIME DP - Unbound Medicine ER -