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Phylogenetic reconstruction of Aegilops section Sitopsis and the evolution of tandem repeats in the diploids and derived wheat polyploids.
Genome. 2006 Aug; 49(8):1023-35.G

Abstract

The evolution of 2 tandemly repeated sequences Spelt1 and Spelt52 was studied in Triticum species representing 2 evolutionary lineages of wheat and in Aegilops sect. Sitopsis, putative donors of their B/G genomes. Using fluorescence in situ hybridization we observed considerable polymorphisms in the hybridization patterns of Spelt1 and Spelt52 repeats between and within Triticum and Aegilops species. Between 2 and 28 subtelomeric sites of Spelt1 probe were detected in Ae. speltoidies, depending on accession. From 8 to 12 Spelt1 subtelomeric sites were observed in species of Timopheevi group (GAt genome), whereas the number of signals in emmer/aestivum accessions was significantly less (from 0 to 6). Hybridization patterns of Spelt52 in Ae. speltoides, Ae. longissima, and Ae. sharonensis were species specific. Subtelomeric sites of Spelt52 repeat were detected only in T. araraticum (T. timopheevii), and their number and chromosomal location varied between accessions. Superimposing copy number data onto our phylogenetic scheme constructed from RAPD data suggests 2 major independent amplifications of Spelt52 and 1 of Spelt1 repeats in Aegilops divergence. It is likely that the Spelt1 amplification took place in the ancient Ae. speltoides before the divergence of polyploid wheats. The Spelt52 repeat was probably amplified in the lineage of Ae. speltoides prior to divergence of the allopolyploid T. timopheevii but after the divergence of T. durum. In a separate amplification event, Spelt52 copy number expanded in the common ancestor of Ae. longissima and Ae. sharonensis.

Authors+Show Affiliations

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia. salina@bionet.nsc.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17036077

Citation

Salina, Elena A., et al. "Phylogenetic Reconstruction of Aegilops Section Sitopsis and the Evolution of Tandem Repeats in the Diploids and Derived Wheat Polyploids." Genome, vol. 49, no. 8, 2006, pp. 1023-35.
Salina EA, Lim KY, Badaeva ED, et al. Phylogenetic reconstruction of Aegilops section Sitopsis and the evolution of tandem repeats in the diploids and derived wheat polyploids. Genome. 2006;49(8):1023-35.
Salina, E. A., Lim, K. Y., Badaeva, E. D., Shcherban, A. B., Adonina, I. G., Amosova, A. V., Samatadze, T. E., Vatolina, T. Y., Zoshchuk, S. A., & Leitch, A. R. (2006). Phylogenetic reconstruction of Aegilops section Sitopsis and the evolution of tandem repeats in the diploids and derived wheat polyploids. Genome, 49(8), 1023-35.
Salina EA, et al. Phylogenetic Reconstruction of Aegilops Section Sitopsis and the Evolution of Tandem Repeats in the Diploids and Derived Wheat Polyploids. Genome. 2006;49(8):1023-35. PubMed PMID: 17036077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogenetic reconstruction of Aegilops section Sitopsis and the evolution of tandem repeats in the diploids and derived wheat polyploids. AU - Salina,Elena A, AU - Lim,K Yoong, AU - Badaeva,Ekaterina D, AU - Shcherban,Andrey B, AU - Adonina,Irina G, AU - Amosova,Alexandra V, AU - Samatadze,Tatiana E, AU - Vatolina,Tatyana Yu, AU - Zoshchuk,Sviatoslav A, AU - Leitch,Andrew R, PY - 2006/10/13/pubmed PY - 2007/1/26/medline PY - 2006/10/13/entrez SP - 1023 EP - 35 JF - Genome JO - Genome VL - 49 IS - 8 N2 - The evolution of 2 tandemly repeated sequences Spelt1 and Spelt52 was studied in Triticum species representing 2 evolutionary lineages of wheat and in Aegilops sect. Sitopsis, putative donors of their B/G genomes. Using fluorescence in situ hybridization we observed considerable polymorphisms in the hybridization patterns of Spelt1 and Spelt52 repeats between and within Triticum and Aegilops species. Between 2 and 28 subtelomeric sites of Spelt1 probe were detected in Ae. speltoidies, depending on accession. From 8 to 12 Spelt1 subtelomeric sites were observed in species of Timopheevi group (GAt genome), whereas the number of signals in emmer/aestivum accessions was significantly less (from 0 to 6). Hybridization patterns of Spelt52 in Ae. speltoides, Ae. longissima, and Ae. sharonensis were species specific. Subtelomeric sites of Spelt52 repeat were detected only in T. araraticum (T. timopheevii), and their number and chromosomal location varied between accessions. Superimposing copy number data onto our phylogenetic scheme constructed from RAPD data suggests 2 major independent amplifications of Spelt52 and 1 of Spelt1 repeats in Aegilops divergence. It is likely that the Spelt1 amplification took place in the ancient Ae. speltoides before the divergence of polyploid wheats. The Spelt52 repeat was probably amplified in the lineage of Ae. speltoides prior to divergence of the allopolyploid T. timopheevii but after the divergence of T. durum. In a separate amplification event, Spelt52 copy number expanded in the common ancestor of Ae. longissima and Ae. sharonensis. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/17036077/Phylogenetic_reconstruction_of_Aegilops_section_Sitopsis_and_the_evolution_of_tandem_repeats_in_the_diploids_and_derived_wheat_polyploids_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/g06-050?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -